A study of hybrid organic/inorganic hydrogel films based on in situ-generated TiO2 nanoparticles and methacrylated gelatin

Abstract

Methacrylated gelatin films with in situ-generated TiO 2 nanoparticles containing varying weight percentages of gelatin (0 %, 0.5 %, 1 %, 2 % and 4 %) were successfully prepared as novel biomaterials. 1 H-NMR spectroscopy confirmed their methacrylation with a 79 % degree of substitution. TiO 2 nanoparticles were uniformly distributed in the films with the average particle size increasing from 85 to 130 nm in proportion to an increase in TiO 2 concentration from 0.5 to 4 wt%. The water absorption of various gelatin methacrylamide/TiO 2 films was in the range of 471-758 %, which was enough to prevent wound beds from exudates accumulation. And in vitro degradation test in PBS showed that the three-dimensional structure of all samples basically remained unchanged although more than or nearly half the mass of specimens decreased after 4 weeks' degradation, and the pH levels of all sample solutions were maintained in an adequate range of 6.5-7.4 for cell and tissue growth during the whole process. The antibacterial activities of the films against E. coli and S. aureus were measured via a shake flask test and demonstrated good performance after the importation of TiO 2 nanoparticles. Cytotoxicity testing revealed that all films had no cytotoxicity and showed favorable adherence in the presence of L929 cells. The results suggest that hybrid hydrogel films hold potential for antibacterial wound dressing and tissue engineering scaffold applications.

Printability of Methacrylated Gelatin upon Inclusion of a Chloride Salt and Hydroxyapatite Nano‐Particles

“…Upon adding up to 1.11 m NaCl salt or 100 m m CaCl 2 salt, the GelMA molecules likely experienced some transition from the extended conformation to a more random coil conformation. This increased conformational freedom in turn increased the mobility of the previously buried peptide bonds and amino acid side chains and allowed GelMA to interact more freely with water toward a “salting in” effect . Adding only 50 m m of either chloride salt resulted in the zeta potential being neutralized; this likewise infers a change to the GelMA's conformation even at such a low chloride salt concentration.…”

“…In addition, with the ultimate goal of developing constructs engineered to match (and replace) natural tissues, such as articular cartilage and bone, this organic material and its composites have not yet come close to achieving the requisite mechanical properties . In this study, we sought to address both of these previously unmet needs by achieving a higher volume fraction of gelatin in the ink, adding a controlled amount of chloride salt, and adding a volume fraction of reinforcing phase that is greater than has typically been pursued in this combination . These three approaches notably contributed to the development of an advanced functional biomaterial ink more suitable for biofabrication.…”

Structure and Rheology of Poloxamine T1107 and Its Nanocomposite Hydrogels with Cyclodextrin-Modified Barium Titanate Nanoparticles

“…21 A substantial amount of work is focusing on inorganic nanoparticles and ceramics as bioactive components in bioengineering, 22,23 such as bioactive silica nanoparticles for improved osteogenesis 24,25 or TiO2 for antibacterial biodegradable hydrogels. 26 In this work, we focus on Barium Titanate (BaTiO3, BT) nanoparticles, a perovskite-type ferroelectric ceramic that possesses a high dielectric constant with piezoelectric properties (in its tetragonal, orthorhombic and rhombohedral crystalline phases). BT has been replaced in some -mainly electric -applications by multiceramic materials, such as lead zirconate titanates, or PZTs.…”

Physico‐mechanical, thermal, and ultraviolet light barrier properties of collagen hydrolysate films from leather solid wastes incorporated with nano TiO2

“…However, dispersion of TiO 2 NPs at the higher loading levels (2%) was not uniform, and some agglomeration was observed. This result might be due to the small TiO 2 NPs with high surface energy congregate to large agglomerates at high concentrations …”

“…TiO 2 is a characteristic filler and exists in three crystalline forms; anatase, rutile, and brookite, and is obtained in different sizes, shapes, and surface features . Due to the small size and high surface area, TiO 2 NPs possess unique physical and chemical properties and can interact with the polymer to enhance the mechanical and physical properties of polymers …”