A study of matrix metalloproteinase expression and activity in atopic dermatitis using a novel skin wash sampling assay for functional biomarker analysis

Harper, John; Godwin, Heather; Green, A.; Wilkes, L.E.; Holden, NS; Moffatt, Miriam F.; Cookson, William; Layton, Guy T.; Chandler, Stephen F.

doi:10.1111/j.1365-2133.2009.09467.x

Cited by 71 publications

(71 citation statements)

References 49 publications

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“…Up-regulation of MMP-9 expression in the inflamed area of the skin may lead to the degradation of extracellular matrix proteins, resulting in increased infiltration of immune cells into the skin. Previous studies have shown that the activity of MMPs such as MMP-9 is increased in skin wash samples from AD lesions (6). ROS is considered to play a major role in inflammation processes during the development of inflammatory skin diseases (21).…”

Section: Discussionmentioning

confidence: 99%

“…MMP-9 can mediate the degradation of extracellular matrix proteins such as type IV collagen during the inflammatory response. It has been implied that dysregulation of MMP activity is associated with inflammatory skin diseases such as atopic dermatitis (AD) (5,6). Previous studies reported that elevated levels of MMP-9 are present in the plasma of patients with AD (5).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies reported that elevated levels of MMP-9 are present in the plasma of patients with AD (5). Recently, it was demonstrated that various MMPs such as MMP-8 and 9 exhibit increased activity in skin wash samples from AD lesions (6), suggesting that MMP activity contributes to the inflammatory process in AD. Stimulation of keratinocytes with tumor necrosis factor-α (TNF-α) results in activation of NF-κB, which is responsible for up-regulation of MMP-9 (7,8).…”

Section: Introductionmentioning

confidence: 99%

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Suppression of TNF-alpha-induced MMP-9 expression by a cell-permeable superoxide dismutase in keratinocytes

Song

Ju²,

Goh³

et al. 2011

BMB Reports

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Suppression of TNF-alpha-induced MMP-9 expression by a cell-permeable superoxide dismutase in keratinocytes

Song

Ju²,

Goh³

et al. 2011

BMB Reports

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“…Quite recently, Harper et al have revealed that the activity of MMPs in skin lesions associated with atopic dermatitis is approximately five-fold higher than in unaffected regions and as many as 10 to 24 times higher than in the healthy individuals. Furthermore, elevated levels of MMP-8 and MMP-9 were documented in atopic dermatitis patients, while the levels of MMP-10, TIMP-1, and TIMP-2 were markedly lower than in healthy individuals [27]. Also, Devillers et al observed an elevated serum level of MMP-9 in atopic dermatitis patients as compared to a group of healthy individuals [28].…”

Section: A At To Op Pi Ic C D De Er Rm Ma At Ti It Ti Is Smentioning

confidence: 97%

The role of extracellular matrix metalloproteinases and their inhibitors in allergic diseases

Kuźmiński¹,

Przybyszewski²,

Graczyk³

et al. 2012

pdia

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A b s t r a c tDuring the migration to the site of inflammation, allergic inflammatory cells must overcome several structures that constitute an impregnable barrier under normal conditions. One such structure is the extracellular matrix responsible for closely filling the intercellular space of tissues. The loosening of this tight structure by the activity of specific tissue enzymes, i.e. extracellular matrix metalloproteinases (MMPs), enables the influx of effector cells to the site of inflammation, thus facilitating the development of allergic inflammation and sustaining its presence. Specific tissue inhibitors of metalloproteinases (TIMPs) represent the most important factor involved in the regulation of MMP activity. Under physiological conditions, MMPs and TIMPs co-exist in a particular specific equilibrium, which is disturbed under pathological circumstances. The MMP-2 and MMP-9, referred to as gelatinases, belong to the best-studied MMPs associated with the development of allergic disorders. Their role pertains to the degradation of type IV collagen, the main component of basal membranes; this facilitates the influx of cells to the site of allergic inflammation. K Ke ey y w wo or rd ds s: : extracellular matrix, metalloproteinases, metalloproteinase inhibitors.

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“…Atopic dermatitis is a major degenerative disease, which presents as a chronic inflammatory skin condition with a deficiency in barrier function (16). It is now well recognized that several proteases, including the matrix metalloproteinases (MMPs), play key roles in the immunopathology of and biological functions during progression of the disease (17).…”

Section: Introductionmentioning

confidence: 99%

Mitigation of 2,4-dinitrofluorobenzene-induced atopic dermatitis-related symptoms by Terminalia chebula Retzius

Lee¹

2011

Int J Mol Med

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Abstract.To evaluate whether an aqueous seed extract of Terminalia chebula Retzius inhibited development of atopy in vivo, we used a 2,4-dinitrofluorobenzene (DNFB)-induced animal model of atopic symptoms to investigate the effects of the extract. We measured CD4 + cell numbers by hematoxylin and eosin (H&E) staining, and determined the expression levels of matrix metalloproteinase (MMP)-9, interleukin (IL)-31, and T-bet genes, in this animal model. The data showed that a Terminalia chebula extract (100 µg/ml) exhibited strong anti-atopic activity, mediating a 52% reduction in the immune response, as measured by thickness of ear swelling, and resulting in decreased eosinophil levels in adjacent skin tissue. Collectively, the results indicate that a Terminalia chebula seed extract has potential for alleviation of atopy-like symptoms induced by DNFB in the mouse. IntroductionIt is generally accepted that the daily consumption of plantderived phytochemicals in vegetables, fruit, tea or herbal extracts may mitigate free-radical attacks (1-3). Terminalia chebula Retzius is an ethnopharmacological plant of India and Southeast Asia, and has traditionally been used as a laxative, a diuretic, and an anti-oxidative material (4-8). In addition, plant extracts have antibacterial (9,10), antiviral (11), antifungal (12) and immune modulatory activities (13,14). Recently, Lee et al (5,15) reported that an aqueous seed extract of Terminalia chebula Retzius (TcRSE) had potent anti-oxidant activity both in vitro and in vivo when tested as a hepatoprotective agent in an animal model. Atopic dermatitis is a major degenerative disease, which presents as a chronic inflammatory skin condition with a deficiency in barrier function (16). It is now well recognized that several proteases, including the matrix metalloproteinases (MMPs), play key roles in the immunopathology of and biological functions during progression of the disease (17). With limited exceptions, normal healthy skin does not contain or exhibit high levels of MMP activity. Thus, evaluation of enzyme activities is important in detection, prior to mitigation, of allergic reactions (18,19). However, little effort has been devoted to treatment of atopy-related disorders with natural extracts, and we thus prioritized the investigation of TcRSE as an inhibitor of anti-atopic dermatitis activity.In the present study, we initially sought immunomodulatory components in natural resources including foods and oriental herbs, and found that TcRSE was active in vitro (data not shown). We thus examined the ameliorating effects of TcRSE against 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis in mice. Biochemical and immunochemical analyses, including cytokine expression evaluation and phenotypic atopic symptoms, were performed to investigate whether TcRSE alleviated the disorder. Materials and methodsChemicals. MMP-9, anti-T-bet and interleukin (IL)-31 antiinterleukin (IL)-31 anti-IL)-31 antibodies were purchased from Cell Signaling (catalog no. 3852; Danvers, MA), Santa Cruz ...

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A study of matrix metalloproteinase expression and activity in atopic dermatitis using a novel skin wash sampling assay for functional biomarker analysis

Cited by 71 publications

References 49 publications

Suppression of TNF-alpha-induced MMP-9 expression by a cell-permeable superoxide dismutase in keratinocytes

Suppression of TNF-alpha-induced MMP-9 expression by a cell-permeable superoxide dismutase in keratinocytes

The role of extracellular matrix metalloproteinases and their inhibitors in allergic diseases

Mitigation of 2,4-dinitrofluorobenzene-induced atopic dermatitis-related symptoms by Terminalia chebula Retzius

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