A Study of Nanomaterial Dispersion in Solution by Wet-Cell Transmission Electron Microscopy

Franks, Ryan; Morefield, Sean; Wen, Jianguo; Liao, Dongxiang; Alvarado, Jorge L.; Strano, Michael S.; Marsh, Charles

doi:10.1166/jnn.2008.18351

Cited by 19 publications

(17 citation statements)

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“…403 SiN window grids were fabricated by binding commercially available SiN wafers with polymer O-rings. 404 The polymers greatly simplified the cell fabrication, decreasing the assembly times down to 10 to 15 min, and allowing the re-use of the wet cells. SiN TEM grids are currently commercially available with electrochemistry and heating packages, making them the most popular option among the different TEM liquid cells.…”

Section: Microscopy Techniques For Np Characterizationmentioning

confidence: 99%

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

2018

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Nanostructures have attracted huge interest as a rapidly growing class of materials for many applications. Several techniques have been used to characterize the size, crystal structure, elemental composition and a variety of other physical properties of nanoparticles. In several cases, there are physical properties that can be evaluated by more than one technique. Different strengths and limitations of each technique complicate the choice of the most suitable method, while often a combinatorial characterization approach is needed. In addition, given that the significance of nanoparticles in basic research and applications is constantly increasing, it is necessary that researchers from separate fields overcome the challenges in the reproducible and reliable characterization of nanomaterials, after their synthesis and further process (e.g. annealing) stages. The principal objective of this review is to summarize the present knowledge on the use, advances, advantages and weaknesses of a large number of experimental techniques that are available for the characterization of nanoparticles. Different characterization techniques are classified according to the concept/group of the technique used, the information they can provide, or the materials that they are destined for. We describe the main characteristics of the techniques and their operation principles and we give various examples of their use, presenting them in a comparative mode, when possible, in relation to the property studied in each case.

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Section: Microscopy Techniques For Np Characterizationmentioning

confidence: 99%

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

2018

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“…2A. 31 The design utilizes SN window wafer grids readily available in the market, instead of homemade ones. A custombuilt metal holder was used to hold the grids, and several O-rings were used to seal the liquid from the vacuum in the TEM chamber.…”

Section: Major Technical Trends Of In Situ Wet Cell Temsmentioning

confidence: 99%

“…A JEOL 2010 LaB 6 TEM system was used to test the design; moreover, different samples, such as Al 2 O 3 nanoparticles in DI water and multi-walled carbon nanotubes in DI water, were imaged, demonstrating the versatility and reliability of the technique. 31 The image resolution in the abovementioned trends is limited by the thickness of the SN window and the liquid layer, mainly due to electron scattering. 32 Another major trend is using low vapor pressure liquids such as ionic liquids (ILs), 33 and studying the liquid-solid systems in TEM.…”

Section: Major Technical Trends Of In Situ Wet Cell Temsmentioning

confidence: 99%

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Recent developments of the in situ wet cell technology for transmission electron microscopies

2015

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In situ wet cells for transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM) allow studying structures and processes in a liquid environment with high temporal and spatial resolutions, and have been attracting increasing research interests in many fields. In this review, we highlight the structural and functional developments of the wet cells for TEM and STEM. One of the key features of the wet cells is the sealing technique used to isolate the liquid sample from the TEM/STEM vacuum environments, thus the existing in situ wet cells are grouped by different sealing methods. In this study, the advantages and shortcomings of each type of in situ wet cells are discussed, the functional developments of different wet cells are presented, and the future trends of the wet cell technology are addressed. It is suggested that in the future the in situ wet cell TEM/STEM technology will have an increasing impact on frontier nanoscale research.

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“…We have also used polystyrene microspheres at the corners of a microchip as a spacer (de Jonge et al, 2009). If flow is not needed, then a sealed device can be used (de Jonge et al, 2007; Franks et al, 2008; Liu et al, 2008; Peckys et al, 2009; Huang et al, 2012). Others have included an additional liquid reservoir in the microchip assembly (Zheng et al, 2009 b ).…”

Section: Liquid Stemmentioning

confidence: 99%

Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells

Peckys

Jonge

2014

Microsc Microanal

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Scanning transmission electron microscopy (STEM) of specimens in liquid, so-called Liquid STEM, is capable of imaging the individual subunits of macromolecular complexes in whole eukaryotic cells in liquid. This paper discusses this new microscopy modality within the context of state-of-the-art microscopy of cells. The principle of operation and equations for the resolution are described. The obtained images are different from those acquired with standard transmission electron microscopy showing the cellular ultrastructure. Instead, contrast is obtained on specific labels. Images can be recorded in two ways, either via STEM at 200 keV electron beam energy using a microfluidic chamber enclosing the cells, or via environmental scanning electron microscopy at 30 keV of cells in a wet environment. The first series of experiments involved the epidermal growth factor receptor labeled with gold nanoparticles. The labels were imaged in whole fixed cells with nanometer resolution. Since the cells can be kept alive in the microfluidic chamber, it is also feasible to detect the labels in unfixed, live cells. The rapid sample preparation and imaging allows studies of multiple whole cells.

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A Study of Nanomaterial Dispersion in Solution by Wet-Cell Transmission Electron Microscopy

Cited by 19 publications

References 0 publications

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

Recent developments of the in situ wet cell technology for transmission electron microscopies

Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells

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