A study of noncovalent protein complexes by matrix-assisted laser desorption/ionization

Detection of Amyloid Beta (Aβ) Oligomeric Composition Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS)

“…Effects of different TFA concentrations in the matrix solution was assessed, and we concluded that a reduction in TFA concentrations was most beneficial in the detection of oligomers within the sample. This coincides with previous studies indicating low pH disruptions in the formation of non-covalent complexes [55]. The instrument's acquisition parameters were set to optimize for detection sensitivity.…”

“…Hence, a common concern is the possibility of artificial non-specific associations that could occur either during sample spot drying or within the mass spectrometer in gas phase. In the literature, numerous examples of MALDI MS application on complexes of biomolecules were reported and reviewed [55][56][57]. The consensus is that the technique is capable of detecting certain non-covalent multimeric complexes under optimized conditions.…”

Strong ionic interactions in noncovalent complexes between poly(ethylene imine), a cationic electrolyte, and Cibacron Blue, a nucleotide mimic – implications for oligonucleotide vectors

“…Mass spectrometry (MS) has become a powerful tool for the elucidation of large biomolecules and their noncovalent complexes after soft ionization techniques, especially electrospray ionization (ESI) and matrix‐assisted laser desorption/ionization (MALDI), have made possible the generation of gas‐phase ions from such species . These ionization techniques have also resulted in widespread MS applications to polymer research, where MS is vital for the characterization of the chemical compositions and functionality distributions of synthetic polymers .…”

“…[22] Mass spectrometry (MS) has become a powerful tool for the elucidation of large biomolecules and their noncovalent complexes after soft ionization techniques, especially electrospray ionization (ESI) [25] and matrix-assisted laser desorption/ionization (MALDI), [26,27] have made possible the generation of gas-phase ions from such species. [28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43] These ionization techniques have also resulted in widespread MS applications to polymer research, where MS is vital for the characterization of the chemical compositions and functionality distributions of synthetic polymers. [44][45][46][47][48] Examination of the noncovalent complexes between synthetic polymers and other (bio)molecules by MS is a nascent field that could nevertheless develop into a viable means for the determination of the stoichiometries and structural properties of these compositionally complex systems.…”

Nanodiamonds Used as a Platform for Studying Noncovalent Interaction by MALDI‐MS

“…5 Thus, in order to improve the preference of MALDI in the noncovalent interaction study, many researchers have applied themselves to less the negative effects of matrix and laser, such as changing laser-matrix combinations and varying sample preparation conditions. [5][6][7] An alternative strategy is using a cross-linking agent to convert the noncovalent into the covalent binding, which is more time-consuming and precious. 4 Here a different approach of "Bait and Fish" to study noncovalent interactions by MALDI-MS has been reported, in which the "fish analyte" is separated from the solution by "bait-bound" nanodiamonds and further analyzed by MALDI-MS directly.…”