A Study of Short- and Long-term mRNA Levels of the <i>Retn, Iapp</i>, and <i>Drd5</i> Genes in Obese Mice Induced with High-fat Diet

Timirci-Kahraman, Özlem; Yılmaz, Ümit; Yilmaz, Nesibe; Çevik, Aydın; Horozoglu, Cem; Çelik, Faruk; Gökçe, Muhammed Oğuz; Ergen, Arzu; Melekoglu, Abdullah; Zeybek, Ümit

doi:10.21873/invivo.11312

Cited by 2 publications

(1 citation statement)

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“…Dopamine receptor domain 5 (Drd5) genes were previously suggested to contribute to the adipogenesis. Knockdown of dopamine receptor D2 upregulates the expression of adipogenic genes in mouse primary mesencephalic neurons [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of DNA methylation as well as mico-RNA expression and screening of epigenetic markers in adipogenesis

et al. 2022

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This study aimed to use bioinformatics methods to characterize epigenetic changes in terms of micro-RNA(miRNA) expression and DNA methylation during adipogenesis. The mRNA and miRNA expression microarray and DNA methylation dataset were obtained from the GEO database. Differentially expressed genes (DEGs), differentially expressed miRNAs (DEMs) and differentially methylated probes (DMPs) were filtered using the limma package. The R language cluster profile package was used for functional and enrichment analysis. A protein–protein interaction (PPI) network was constructed using STRING and visualized in Cytoscape. The Connection map (CMap) website tool was used to screen potential therapeutic drugs for adipogenesis. When comparing the early and late stages of adipogenesis, 111 low miRNA targeted upregulated genes and 64 high miRNA targeted downregulated genes were obtained, as well as 663 low-methylated high-expressed genes and 237 high-methylated low-expressed genes. In addition, 41 genes (24 upregulated and 17 downregulated) were simultaneously regulated by abnormal miRNA changes and DNA methylation. Ten chemicals were identified as putative therapeutics for adipogenesis. In addition, among the dual-regulated genes identified, CANX, HNRNPA1, MCL1, and PPIF may play key roles in the epigenetic regulation of adipogenesis and may serve as aberrant methylation or miRNA targeting biomarkers.

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Section: Discussionmentioning

confidence: 99%

Characterization of DNA methylation as well as mico-RNA expression and screening of epigenetic markers in adipogenesis

et al. 2022

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Genetic dietary adaptation in Neandertal, Denisovan and Sapiens revealed by gene copy number variation

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Condémi

et al. 2021

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Dietary adaptation is the acquisition of an efficient system to digest food available in an ecosystem. To find the genetic basis for human dietary adaptation, we searched 16 genomes from Neandertal, Denisovan and Early Sapiens for food digestion genes that tend to have more or fewer copies than the modern human reference genome. Here, we identify 11 genes, including three gene clusters, with discernible copy number variation trends at the population level. The genomic variation shows how metabolic pathways for lipid, brown fat, protein or carbohydrate metabolism adapt to metabolize food from animal or plant sources. Interpreting the copy number profiles in relation to fossil evidence shows that Homo sapiens had an evolutionary advantage compared to Neandertal and Denisovan in adapting to cold and temperate ecosystems.

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A Study of Short- and Long-term mRNA Levels of the Retn, Iapp, and Drd5 Genes in Obese Mice Induced with High-fat Diet

Abstract: Our study demonstrated that the mRNA levels of the genetic markers considered to play a role in adipogenesis were different in short- and long-term obesity models formed in C57BL/6J mice using HFD.

Cited by 2 publications

References 30 publications

Characterization of DNA methylation as well as mico-RNA expression and screening of epigenetic markers in adipogenesis

Characterization of DNA methylation as well as mico-RNA expression and screening of epigenetic markers in adipogenesis

Genetic dietary adaptation in Neandertal, Denisovan and Sapiens revealed by gene copy number variation

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