A study of signal transduction for the two diuretic peptides of Diploptera punctata

Tobe, Stephen S.; Zhang, J.R.; Schooley, David A.; Coast, Geoffrey M.

doi:10.1016/j.peptides.2004.07.013

Cited by 17 publications

(5 citation statements)

References 20 publications

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“…We suspect that the phosphorylation of Rab8 in insects is involved in the development of cell polarity in the eye, asymmetric cell division, and Archives of Insect Biochemistry and Physiology October 2007 doi: 10.1002/arch. signal transduction by diuretic peptides by changing the cellular distribution of Rab8, because these processes are regulated by PKC (Betschinger et al, 2005;Djiane et al, 2005;von Stein et al, 2005, Tobe et al, 2005. In further studies, we will use the BRab8 antibody to examine the cellular distribution of BRab8 in the brain of B. mori.…”

Section: Discussionmentioning

confidence: 99%

“…Genetic approaches indicate that insect PKCs are involved in learning and behavior (Humphries et al, 2003;Choi et al, 1991). Also, recent reports indicate that PKC plays a role in the development of cell polarity in the eye, asymmetric cell division, and signal transduction by diuretic peptides (Betschinger et al, 2005;Djiane et al, 2005;von Stein et al, 2005;Tobe et al, 2005). Rab8 from Bombyx mori (BRab8) has recently been shown to be phosphorylated in vitro by B. mori PKC expressed in mammalian cells and by protein kinases purified from the brain of B. mori (Uno et al, 2004(Uno et al, , 2006.…”

Section: Introductionmentioning

confidence: 99%

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Determination of phosphorylated amino acid residues of Rab8 from Bombyx mori

Uno

Nakada

Okamaoto

et al. 2007

Arch Insect Biochem Physiol

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The Rab family of small GTPases are key regulators of membrane trafficking. Partially purified Rab8 from Bombyx mori (BRab8) was phosphorylated by protein kinase C in mammalian cells in vitro. To determine which of the seven serines and four threonines are phosphorylated, we generated deletion and site-directed mutants of BRab8, inserted them in Escherichia coli, partially purified the encoded fusion proteins by affinity chromatography, and examined their phosphorylation by protein kinase C in vitro. We found that Ser-132 of BRab8 was specifically phosphorylated by protein kinase C. In addition, Western blotting using an antiserum against BRab8 and in-gel staining for phosphorylated proteins revealed that BRab8 is phosphorylated in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of phosphorylated amino acid residues of Rab8 from Bombyx mori

Uno

Nakada

Okamaoto

et al. 2007

Arch Insect Biochem Physiol

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“…In both D. melanogaster and A. gambiae ) the native DH 31 -like peptide increases the production of cyclic AMP, while cyclic GMP production is unchanged in the presence of IBMX. Dippu-DH 31 increases the cyclic AMP content of Schistocerca americana tubules, but not tubules of Manduca sexta (Furuya et al, 2000) or of D. punctata (Tobe et al, 2005). Interestingly, Rhopr/Dippu-DH 31 does not elevate the cyclic AMP content of R. prolixus tubules, despite being capable of increasing secretion 14-fold.…”

Section: A Te Brugge D a Schooley And I Orchardmentioning

confidence: 90%

Amino acid sequence and biological activity of a calcitonin-like diuretic hormone (DH31) from Rhodnius prolixus

Brugge

Schooley

Orchard

2008

Journal of Experimental Biology

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SUMMARYDiuresis in the blood-gorging hemipteran Rhodnius prolixus is under neurohormonal control and involves a variety of processes and tissues. These include ion and water movement across the epithelium of the crop and the Malpighian tubules, and muscle contractions of the crop, hindgut and dorsal vessel, which facilitate mixing of the blood-meal, mixing of the haemolymph, as well as the expulsion of waste. One of the neurohormones that might play a role in this rapid diuresis belongs to the calcitonin-like diuretic hormone (DH 31 ) family of insect peptides. Previously we have demonstrated the presence of DH 31 -like peptides in the central nervous system (CNS) and gut of R. prolixus 5th instars. In the present work, a DH 31 from the CNS of 5th instar R. prolixus was isolated using reversed-phase liquid chromatography (RPLC), monitored with an enzyme-linked immunosorbent assay (ELISA) combined with matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry, and sequenced using tandem mass spectrometry and Edman degradation. This neuropeptide is the first to be sequenced in R. prolixus and has a sequence identical to that found previously for Dippu-DH 31 from the cockroach Diploptera punctata. In previous studies testing Rhopr/Dippu-DH 31 in Malpighian tubule secretion assays, we demonstrated increases in the rate of secretion that were small, relative to that induced by serotonin, but nevertheless 14-fold over baseline. In the present study, we investigated second messenger pathways in response to Rhopr/Dippu-DH 31 and found no increase or decrease in cyclic adenosine monophosphate (cyclic AMP) content of the Malpighian tubules. DH 31 -like immunoreactivity is present over the dorsal hindgut, anterior dorsal vessel and dorsal diaphragm, and bioassays of the R. prolixus dorsal vessel and hindgut indicate that Rhopr/Dippu-DH 31 increases the frequency of muscle contractions of both tissues. Second messenger pathways were also investigated for the dorsal vessel and hindgut.

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“…Although CRF-DH plays a pronounced role in the regulation of diuresis, feeding, and reproduction in S. gregaria (Van Wielendaele et al, 2012) and other insects (Audsley et al, 1995(Audsley et al, , 1997Te Brugge and Orchard, 2002;Goldsworthy et al, 2003;Tobe et al, 2005), this is not entirely reflected in the observed tissue distribution, since relative Schgr-CRF-DHR transcript levels are low or undetectable in several tissues that are involved in these processes. This infers that this is probably not the only Schgr-CRF-DH receptor, and/or that the peptide regulates some of these processes in an indirect manner.…”

Section: Tissue Distribution Analysis and Functions Of Schgr-crf-dhmentioning

confidence: 94%

“…(For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) Lee et al, 2016;Reagan, 1996;Tobe et al, 2005). In addition, the production of primary urine is dependent on cAMP, which increases cationic transport (such as K + and Na + ) into the Malpighian tubules (Beyenbach, 1995;O'Donnell et al, 1996).…”

Section: Introductionmentioning

confidence: 99%