A study of sperm acrosin in patients with unexplained infertility

Mohsenian, Mohammad; Syner, Frank N.; Moghissi, Kamran S.

doi:10.1016/s0015-0282(16)46044-9

Cited by 51 publications

(1 citation statement)

References 8 publications

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“…Earlier reports have discussed the value of the acrosin activity test in predicting the IVF success rate, and recent studies have focused on acrosin activity in other species, although not as a diagnostic tool. Contradictory data have been reported regarding the relationships between acrosin activity and conventional semen parameter values, 8 9 10 11 male infertility, 9 10 12 and IVF success rates. 11 13 14 Studies have shown that low acrosin activity is associated with abnormal semen characteristics.…”

Section: Introductionmentioning

confidence: 99%

Sperm acrosin activity may be a useful factor in choosing between ICSI and IVF for infertile male patients

Hu,

Yin

et al. 2023

Asian Journal of Andrology

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The clinical applications of acrosin activity are limited. We analyzed 61 578 male partners in infertile couples who visited the outpatient department of the Reproductive and Genetic Hospital of CITIC-Xiangya (Changsha, China) between August 2014 and December 2019 to determine the reference ranges and thresholds for acrosin activity in infertile Chinese men; to determine whether correlations exist between acrosin activity and age, sperm concentration, sperm morphology, or sperm motility; and to evaluate whether acrosin activity could serve as an effective prognostic indicator for choosing between in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in the clinic. The cut-off value for the normal reference range of acrosin activity for male partners in infertile couples was 24.78 μIU per 106 sperm. There was no significant association between acrosin activity and age, sperm concentration, semen volume, total sperm count, progressive motility, or total motile spermatozoa. A weak positive correlation was found between acrosin activity and normal sperm morphology. There was a statistically significant difference in abnormal acrosome morphology between the group with high acrosin activity (>24.78 μIU per 106 sperm) and the group with low acrosin activity (<24.78 μIU per 106 sperm). The group with a low IVF fertilization rate had a high index of abnormal acrosomal morphology at 21.2%, while the group with a high IVF fertilization rate had a low index of 0.2%. At an acrosin activity of <24.78 µIU per 106 sperm, in one cycle of the same patient, the fertilization rate, normal fertilization rate, and good-quality embryo rate for ICSI were significantly higher than those for IVF. Therefore, the most promising application of acrosin activity could be in the selection of ICSI over IVF for infertile male patients with complete fertilization failure or a low fertilization rate.

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Section: Introductionmentioning

confidence: 99%

Sperm acrosin activity may be a useful factor in choosing between ICSI and IVF for infertile male patients

Hu,

Yin

et al. 2023

Asian Journal of Andrology

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Comparison of methods for detecting mitomycin C‐ and ethyl nitrosourea‐induced germ cell damage in mice: Sperm enzyme activities, sperm motility, testis weight

et al. 1984

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Testes weights, sperm motility and enzyme activities in single sperm were compared with respect to their ability to detect either developmental or mutational damage to germ cells. Male mice were injected i.p. with 2.5 mg/kg mitomycin C (MC) or 50 or 100 mg/kg ethylnitrosourea (ENU) or saline and were then killed at times such that sperm derived from treated vas sperm (SZ), spermatids (ST), preleptotene-late-spermatogonial cells (PLSG), spermatogonial cells (SG), or spermatogonial stem cells (SGS) could be evaluated. Testis weights decreased significantly as early as 1 wk after treatment, with the greatest decrease reached 3-4 wk after treatment, followed by recovery to normal levels 10-15 wk after treatment. We conclude that testis weight, which is easily obtained, is a sensitive indicator of germ cell damage by these agents. Sperm from each animal were evaluated for sperm motility, acrosin activity, succinic dehydrogenase (SDH) activity with or without the competitive inhibitor malonate or after exposure to 60 degrees C for 10 min. The latter two assays were to detect sperm enzymes resistant to the inhibitor or heat. The presence of the acrosin protein was also detected immunologically. Sperm motility decreased most from treatment of PLSG and SG. After MC or ENU treatment, the greatest loss of acrosin activity and of the acrosin protein was also noted in sperm derived from treated PLSG and SG. MC and ENU failed to induce SDH activity in single sperm resistant to 60 degrees C heat inhibition or to inhibition by malonate. Of the sperm assays, acrosin activity proved to be the most sensitive indicator of germ cell damage and was the simplest to measure.

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Comparison of Glycerol and a Zwitter Ion Buffer System as Cryoprotective Media for Human Spermatozoa

Jeyendran

Ven

Kennedy

et al. 1984

Journal of Andrology

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This study compared the cryoprotective effect of glycerol with that of a zwitter ion buffer system (TESTCY). Spermatozoa that are cryopreserved in the presence of glycerol possess a somewhat higher progressive motility immediately after thawing than those preserved in the presence of TESTCY. However, after a 1-hour incubation in glycerol-free medium, the progressive motilities of the glycerol- and TESTCY-treated spermatozoa become essentially identical. After 2 hours in culture medium, TESTCY-treated spermatozoa possess a higher motility than glycerol-treated spermatozoa, indicating that TESTCY is a better preservative than glycerol for the long-term motility of human spermatozoa. The fertilizing potential of the cryopreserved spermatozoa was assessed by their ability to penetrate zona-free hamster oocytes in vitro. Spermatozoa that are cryopreserved in the presence of TESTCY produce three- to four-fold higher penetration rates than glycerol-treated, cryopreserved spermatozoa. Cryopreservation in the presence of TESTCY also results in a higher stability of the acrosin/proacrosin system than when the spermatozoa are preserved in glycerol, since about two- to three-fold higher levels of proacrosin are retained. These results indicate that TESTCY is a better cryopreservative for human spermatozoa than glycerol.

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A study of sperm acrosin in patients with unexplained infertility

Cited by 51 publications

References 8 publications

Sperm acrosin activity may be a useful factor in choosing between ICSI and IVF for infertile male patients

Sperm acrosin activity may be a useful factor in choosing between ICSI and IVF for infertile male patients

Comparison of methods for detecting mitomycin C‐ and ethyl nitrosourea‐induced germ cell damage in mice: Sperm enzyme activities, sperm motility, testis weight

Comparison of Glycerol and a Zwitter Ion Buffer System as Cryoprotective Media for Human Spermatozoa

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