A study of the alkaline ashing method for determination of protein-bound iodine in serum

Foss, Olav; Hankes, L. V.; Slyke, Donald D. Van

doi:10.1016/0009-8981(60)90135-2

Cited by 108 publications

(33 citation statements)

References 14 publications

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“…Thyroid volumes higher than 18 mL were termed enlarged (6). UI excretion was measured in random urine samples using a manual method based on a modification of Barker's dry ash technique (7). Results were expressed directly as microgram of iodine excreted per liter of urine (Mg/L).…”

Section: Methodsmentioning

confidence: 99%

Variation in Iodine Handling During Normal Pregnancy

Smyth¹

1999

Thyroid

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Reports in the literature are divided on changes in thyroid volume and urinary iodine excretion (UI) during normal pregnancy. Reports from Ireland, an area of modest dietary iodine intake (median UI 70 microg/L) showed an increase in UI that rose to a median value of 135 microg/L in the first trimester (T1) and continued at 124 microg/L in the second (T2) and 122 microg/L in the third trimester (T3). In parallel with the increase in UI, mean ultrasound measured thyroid volume increased by a maximum of 47% over nonpregnant values in the third trimester (T3). Although these findings were consistent with studies in Cardiff, UK (median UI 73 microg/L), which also showed a pregnancy-associated rise in UI excretion (maximum 176 microg/L) accompanied by a 30% increase in median thyroid volume, they differed from findings in Sri Lanka (median UI 146 microg/L), a country in which a successful program of salt iodination has recently been implemented, which showed no significant changes in UI excretion (T3 maximum 154 microg/L) but did show a modest (20%) maximum increase in median thyroid volume at T3. Prospective studies on Irish subjects showed that median UI fell precipitously to nonpregnant control values (76 microg/L) at delivery. In addition, UI in neonates sampled at 3-days postdelivery showed that excretion was greater in breast-fed than in bottle fed infants. Differences in reported UI excretion patterns during pregnancy may may reflect the existence of a threshold above which increased renal clearance results in increased iodine loss but that is masked at higher iodine intakes. Assuming constant dietary iodine intake during pregnancy, any increased urine loss will inevitably lead to negative iodine balance and thyroid depletion. In these circumstances, increased thyroid volume may in part be a compensatory mechanism to allow for greater iodine storage.

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Section: Methodsmentioning

confidence: 99%

Variation in Iodine Handling During Normal Pregnancy

Smyth¹

1999

Thyroid

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“…Iodine was determined in blood samples using the Sandell-Kolthoff reaction. Iodine was measured by its catalytic effect on the reduction of Ce(IV) to Ce(III) by As(III), the rate of reduction being followed by the rate of disappearance of the yellow color of the ceric ion [17].…”

Section: Determination Of Serum Iodine Levelmentioning

confidence: 99%

Iodine and Magnesium Levels in Maternal and Umbilical Cord Blood of Preeclamptic and Normal Pregnant Women

Borekci

Gülaboğlu

Gül

2008

Biol Trace Elem Res

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The objective of this study was to investigate the relationship between preeclampsia and iodine levels and magnesium concentration in the blood of subjects in the northeast Anatolia region where iodine deficiency is common. Blood specimens were obtained from 24 preeclamptic and 16 healthy pregnant women. Iodine levels in blood were determined by the Foss method based on the Sandell-Kolthoff reaction. Serum protein-bound iodine (PBI) levels and magnesium concentration in maternal blood were lower in patients with severe preeclampsia compared to normal pregnant women (8.46 +/- 1.22 vs. 11.46 +/- 1.71 microg/dL, p < 0.001, 1.63 +/- 0.05 vs. 1.86 +/- 0.05 mg/dL, p < 0.001, respectively). Serum PBI levels and magnesium concentration in umbilical cord blood were higher in patients with severe preeclampsia than in normal pregnant women (8.84 +/- 1.9 vs. 7.33 +/- 1.07 microg/dL, p < 0.05, 2.48 +/- 0.03 vs. 2.02 +/- 0.01 mg/dL, p < 0.001, respectively). There was a positive correlation between the serum PBI levels in maternal blood and magnesium concentration in maternal blood in patients with severe preeclampsia (r = 0.41, p < 0.05). Thus, iodine may be one factor contributing to the pathophysiology of preeclampsia. Iodine supplementation may be effective therapy in preeclamptic in pregnant women.

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“…Iodine was determined on a water extract of the ash by its catalytic effect on Ce 4+ to Ce+++ by arsenious acid. The optimum conditions worked out by Foss, Hankes, and Van Slyke (1960) were used. Disposable plastic pots (Staynes Laboratories Ltd.) were used for this colour reduction.…”

Section: Serum Protein-hound-iodine Determinationmentioning

confidence: 99%

Comparative Study of Thyroxine Assays Employing Kit Radio-Ligand Reagents

Watson

Lees

1973

Ann Clin Biochem

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A comparative study of some ‘kit’ radiometric procedures for the determination of thyroxine in serum is reported. Four manufacturers' competitive-binding reagents and methods—three incorporating prior extraction and one employing column elution—are examined. Considerable discrepancies exist between the manufacturers' ‘standards’ which, in the main, account for the differences in results obtained by the different methods. Other deficiencies include susceptibility to metal ion interference, variation (up to 8%) in thyroxine extraction and non-specific, i.e. ‘mimic T-4’, measurement. Radio-ligand reagent ‘kits’ need to be critically assessed before being used routinely. Separation of hypo-and hyperthyroid from euthyroid patients is achieved as well with some competitive protein-binding T-4 methods as with serum PBI.

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A study of the alkaline ashing method for determination of protein-bound iodine in serum

Cited by 108 publications

References 14 publications

Variation in Iodine Handling During Normal Pregnancy

Variation in Iodine Handling During Normal Pregnancy

Iodine and Magnesium Levels in Maternal and Umbilical Cord Blood of Preeclamptic and Normal Pregnant Women

Comparative Study of Thyroxine Assays Employing Kit Radio-Ligand Reagents

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