A study of the antigenic composition of the fragilis group of Bacteroides

Elhag, K. M.; Alkarmi, T.

doi:10.1099/00222615-35-2-118

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…Rabbit hyperimmune sera was raised against 22 distinct serotypes of the fragilis group of Bacteroides and used to analyse strains by SDS-PAGE and immunoblotting (18). Cluster analysis of the various bands showed similarities between strains from certain geographical areas at 70-78 %.…”

Section: Sds-page and Immunoblottingmentioning

confidence: 99%

Epidemiological aspects of infections caused byBacteroides fragilis andClostridium difficile

Tabaqchali

Wilks

1992

Eur. J. Clin. Microbiol. Infect. Dis.

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Bacteroides fragilis and Clostridium difficile are two of the most common anaerobes associated with human disease. Studies on the epidemiology of Bacteroides fragilis are limited and are based predominantly on serogrouping, which suggests intraspecies differences. Further studies using newer techniques for typing are required to elucidate the epidemiological characteristics of this important pathogen. By contrast, numerous phenotypic, immunological and molecular methods have been developed for typing and fingerprinting of Clostridium difficile and applied in epidemiological studies to show conclusively that Clostridium difficile is nosocomially acquired and that there is transmission and cross-infection between hospital patients.

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Section: Sds-page and Immunoblottingmentioning

confidence: 99%

Epidemiological aspects of infections caused byBacteroides fragilis andClostridium difficile

Tabaqchali

Wilks

1992

Eur. J. Clin. Microbiol. Infect. Dis.

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Recognition of immunologically important antigens in Bacteroides fragilis infection ?an experimental study in mice

Khanna¹,

Panigrahi²,

Ganguly³

et al. 1994

World J Microbiol Biotechnol

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When the outer membrane proteins (OMP) of Bacteroides fragilis ATCC 23745 grown under seven different culture conditions were analysed by SDS-PAGE, five of the seven preparations showed similar protein profiles. When the three different OMP preparations were subsequently analysed by Western blotting, that from B. fragilis grown in brain heart infusion broth supplemented with haemin and menadione alone showed more immunodominant bands than those grown with additional cholesterol and methionine or cholesterol and cysteine. Only the former showed immunodominant proteins around 45.6 kDa and 66.2 kDa throughout the course of infection in mice. A protein corresponding to 97.4 kDa was found under all culture conditions. The results emphasise the importance of culture conditions in the expression of immunologically reactive antigens and also the possible use of such antigens in the serodiagnosis of B. fragilis infection.

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Detection of Bacteroides fragilis in clinical specimens by polymerase chain reaction amplification of the neuraminidase gene

Jotwani¹,

Kato²,

Kato³

et al. 1995

Current Microbiology

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The polymerase chain reaction (PCR) was used in an attempt to detect Bacteroides fragilis by amplifying a segment of the gene encoding B. fragilis neuraminidase. Forty-five reference strains representing 45 species and 113 clinical isolates were tested. Only B. fragilis was PCR positive, except for Bacteroides merdae ATCC 43184, which gave a band by ethidium bromide staining that showed no signal by Southern hybridization. Using a protocol that employed DNA extraction by Sepa Gene kit and a highly sensitive digoxigenin-chemiluminescence detection system, detection of B. fragilis by PCR was in complete agreement with culture results for 44 clinical specimens from which a wide range of aerobic and anaerobic organisms and fungi were recovered.

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A study of the antigenic composition of the fragilis group of Bacteroides

Cited by 4 publications

References 11 publications

Epidemiological aspects of infections caused byBacteroides fragilis andClostridium difficile

Epidemiological aspects of infections caused byBacteroides fragilis andClostridium difficile

Recognition of immunologically important antigens in Bacteroides fragilis infection ?an experimental study in mice

Detection of Bacteroides fragilis in clinical specimens by polymerase chain reaction amplification of the neuraminidase gene

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