A Study of the Efficiency of Modern Domestic Disinfectants in the System of TB Control Activities

Abstract

Due to the absence of elaborated effi cient means for specifi c prevention of bovine tuberculosis, it is ex- tremely important to detect and eliminate the source of infection and to take veterinary and sanitary preven- tive measures. Here the critical role is attributed to disinfection, which breaks the epizootic chain due to the elimination of pathogenic microorganisms in the environment and involves the application of disinfectants of different chemical groups. Aim. To study the tuberculocidal properties of new disinfectants DZPT-2 and FAG against atypical mycobacteria Mycobacterium fortitum and a TB agent Mycobacterium bovis. Methods. The bacteriological and molecular-genetic methods were used. Results. It was determined that DZPT-2 prepara- tion has bactericidal effect on M. fortuitum when used in the concentration of 2.0 % of the active ingredient (AI) when exposed for 5–24 h, while disinfectant FAG has a bactericidal effect in the concentration of 2.0 % when exposed for 24 h. Disinfectant DZPT-2 in the concentration of 2.0 % of the AI, when exposed for 5–24 h, and FAG preparation in the concentration of 2.0 %, when exposed for 24 h, and with the norm of consump- tion rate of 1 cubic decimeter per 1 square meter disinfect the test-objects (batiste, wood, glazed tile, metal, glass), contaminated with the TB agent M. bovis. Conclusions. Disinfecting preparations of DZPT-2 in the concentration of 2.0 % of AI when exposed for 5 h and FAG in the concentration of 2.0 % when exposed for 24 h may be used in the complex of veterinary and sanitary measures to prevent and control TB of farm ani- mals. The possibility of using the polymerase chain reaction as an additional method of estimating tuberculo- cide activity of disinfectants was proven.

Antibacterial effect of "Ecocide C" disinfectant against mycobacteria

“…При апробації деззасобу щодо збудників туберкульозу M. bovis та M. avium було визначено режими його застосування для знезараження контамінованих об'єктів (5,0 % -24 год), але при цьому необхідно зазначити, що знезаражуюча дія препарату залежить від виду оброблюваних об'єктів, що також підтверджують й інші дослідники (Polakov, 1975). Підсумовуючи результати наших лабораторних досліджень доведено можливість використання ПЛР як додаткового методу оцінки туберкулоцидної активності не лише альдегідних препаратів (Paliy et al, 2015), але й кислотних деззасобів. При порівнянні отриманих нами результатів та існуючих даних щодо режимів застосування препарату «Екоцид С» можна зробити висновок, що між ними існує кореляційна залежність.…”

Evaluation of acute toxicity of the "Orgasept" disinfectant

“…Disinfection is a leading factor in ensuring the epizootic welfare of livestock and obtaining safe products of animal origin of high sanitary quality (Zavgorodniy et al, 2013;Gosling, 2018). Disinfection technologies are especially important in the prevention and control of vaccine-uncontrolled diseases (Paliy et al, 2015;De Lorenzi et al, 2020), and due to the concentration of a significant number of animals in limited areas when transferred livestock on an industrial scale (Stringfellow et al, 2009;Shkromada et al, 2019). Recently, disinfection approach has changed due to latest biocidal products (Paliy et al, 2016;Kovalenko et al, 2018;Stegniy et al, 2019).…”

Virucidal properties of innovative disinfectant to Avian influenza virus and Newcastle disease virus

“…A number of disinfectants that contain different classes of chemical compounds as active substances have been developed and proposed for practical use, and they are recommended for use in different branches of industry (Zavgorodniy and Paliy, 2014;Paliy et al, 2015;Kovalenko, Paliy and Zagrebelniy, 2017;Rodionova and Paliy, 2017). www.jvmbbs.kharkov.ua It should be pointed out that the large-scale production of disinfectants and their introduction into practice is impossible without the preliminary laboratory assessment of their antimicrobial properties, the determination of the spectrum of their biocidal effect and physical, chemical and toxicological properties (Zavgorodniy et al, 2013;Paliy et al, 2016).…”