A study of the heating effect on mouse liver after Cryosurgery

Takahashi, Daishi; Haruki, Nakamura; SHINODA, Kazufumi; Fukumoto, Ichiro

doi:10.1007/978-3-540-36841-0_849

Cited by 1 publication

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pre-freezing tissue followed by RF heating treatment not only directly killed the tumor cells, but the tumor associated immune-suppression environment was also altered and anti-tumor immunity was stimulated [12][13][14]. Takahashi et al [15] have also found improved ablation using similar combination therapies. Further, heating of the prefrozen tissue caused much greater injury to tumor microvasculature, as reported by Shen et al [16,17].…”

Section: Introductionmentioning

confidence: 99%

Study of enhanced radiofrequency heating by pre-freezing tissue

Zhang

Zou

et al. 2018

International Journal of Hyperthermia

View full text Add to dashboard Cite

In our previous animal model study, we found that radiofrequency (RF) ablation of pre-frozen tumor resulted in improved therapeutic effects. To understand the underlying mechanisms and optimize the treatment protocol, the RF heating pattern in pre-frozen tissue was studied in this paper. Both ex vivo and in vivo experiments were conducted to compare the temperature profiles of RF heating with or without pre-freezing. Results showed that the heating rate of in vivo tissues was significantly higher with pre-freezing. However, little difference was observed in the heating rate of ex vivo tissues with or without pre-freezing. In the histopathologic analysis of in vivo tissues, both a larger ablation area and a wider transitional zone were found in the tissue with pre-freezing. To investigate the cause for the enhancement in RF heating, the parameters affecting the tissue temperature rise were studied. It was found that the electrical conductivity of in vivo tissue with pre-freezing was much higher at low frequencies, but little difference was found at the 460 kHz frequency commonly used in clinical applications. A finite element model for RF heating was developed and validated to fit the thermal conductivity of in vivo tissue including effects of pre-freezing and the associated blood perfusion rate. Results showed that the enhancement of the heating rate was primarily attributed to the decreased blood perfusion rate in the tissue with vascular damage caused by pre-freezing. The ablation volume was increased by 104% due to the reduced heat dissipation.

show abstract