A study of the inhibition of jack bean urease by garlic extract

Juszkiewicz, A.; Zaborska, Anna; Łaptaś, Anna; Olech, Zofia

doi:10.1016/j.foodchem.2003.07.033

Cited by 43 publications

(38 citation statements)

References 24 publications

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“…Among the urease-positive strains, P. vulgaris was found to be rapid positive strain while the rest were delayed-positive strains. All the tested urease-positive strains are well known for their role in causing infections in the urinary tract (Juszkiewicz et al, 2004).…”

Section: Urease Testing Assaymentioning

confidence: 99%

“…Staghorn stones, which contain carbonate apatite and monoammonium urate, can become quite large and fill up the entire kidney. These stones are always associated with urinary tract infections with ureasplitting bacteria, namely Proteus sp., K. pneumoniae, P. aeruginosa, and S. aureus (Belhadji et al, 2004;Griffith et al, 1976;Juszkiewicz et al, 2004;Rahman et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Previous literature revealed the isolation of urease inhibitors from some plants and herbs (Ayaz et al, 2006;Shafiq et al, 2011). Typically, Allium sativum L. (Amaryllidaceae) extract is a natural inhibitor of urease (Juszkiewicz et al, 2004). Hypericum oblongifolium Wall.…”

Section: Introductionmentioning

confidence: 99%

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In vitroscreening and evaluation of some Indian medicinal plants for their potential to inhibit Jack bean and bacterial ureases causing urinary infections

Bai

Bharti

Seasotiya

et al. 2014

Pharmaceutical Biology

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ORIGINAL ARTICLEIn vitro screening and evaluation of some Indian medicinal plants for their potential to inhibit Jack bean and bacterial ureases causing urinary infections Sheema Bai, Pooja Bharti, Leena Seasotiya, Anupma Malik, and Sunita Dalal Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India Abstract Context: Bacterial ureases play an important role in pathogenesis of urinary infections. Selection of plants was done on the basis of their uses by the local people for the treatment of various bacterial and urinary infections. Objective: Our investigation screens and evaluates 15 Indian medicinal plants for their possible urease inhibitory activity as well as their ability to inhibit bacteria causing urinary infections. Materials and methods: Plant extracts in three different solvents (methanol, aqueous, and cow urine) were screened for their effect on Jack-bean urease using the phenol-hypochlorite method. Subsequently, seven bacterial strains were screened for their ability to release urease and further antimicrobial-linked urease inhibition activity and minimum inhibitory concentration of the tested extracts were evaluated by the agar well diffusion and microdilution method, respectively. Results: Five plants out of 15 crude extracts revealed good urease inhibitory activity (!20% at 1 mg/ml conc.) and IC 50 values for these extracts ranged from 2.77 to 0.70 mg/ml. Further testing of these extracts on urease-producing bacterial strains (Staphylococcus aureus NCDC 109, S. aureus MTCC 3160, Proteus vulgaris MTCC 426, Klebsiella pneumoniae MTCC 4030, and Pseudomonas aeruginosa MTCC 7453) showed good anti-urease potency with an MIC ranging from 500 to 7.3 mg/ml. Discussion and conclusion: The results of screening as well as susceptibility assay clearly revealed a strong urease inhibitory effect of Acacia nilotica L. (Fabaceae), Emblica officinalis Gaertn. (Phyllanthaceae), Psidium guajava L. (Myrtaceae), Rosa indica L. (Rosaceae), and Terminalia chebula Retz. (Combretaceae). Our findings may help to explain the beneficial effect of these plants against infections associated with the urease enzyme.

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Section: Urease Testing Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitroscreening and evaluation of some Indian medicinal plants for their potential to inhibit Jack bean and bacterial ureases causing urinary infections

Bai

Bharti

Seasotiya

et al. 2014

Pharmaceutical Biology

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“…To date, allicin is the best characterized biologically active compound of GE. Allicin undergoes thiol-disulfide exchange reactions and can react with SH-containing enzymes, causing their inhibition (Wills 1956;Feldberg et al 1988;Focke et al 1990;Ankri et al 1997;Rabinkov et al 1998;Juszkiewicz et al 2004;Zaborska et al 2009). At present, a variety of biological effects of allicin is attributed not only to its high reactivity with SH-containing molecules, but also to its antioxidant activity and its high permeability through membranes (Prasad et al 1995;Rabinkov et al 1998;Miron et al 2000;Pedraza-Chaverri et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of thiosulphinates contained in garlic extract on growth, proton fluxes and membrane potential in maize (Zea mays L.) coleoptile segments

et al. 2011

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The effect of thiosulphinates contained in garlic extract (GE) on endogenous growth, growth in the presence of either indoleacetic acid (IAA) or fusicoccin (FC), and proton extrusion in maize coleoptile segments were studied. In addition, membrane potential changes at some GE dilutions and the protective effect of dithiothreitol (DTT) against GE toxicity were also determined. It was found that GE at almost all dilutions studied, when added to the incubation medium inhibited endogenous growth as well as growth in the presence of either IAA or FC. Simultaneous measurements of growth and external pH indicated that the administration of GE resulted in a complex change in the pH of the external medium; after an initial transient acidification, pH increased and reached the maximal value followed by a gradual decrease of medium pH. When IAA or FC was added after preincubation of the segments in the presence of GE the changes in medium pH were not significantly different from these obtained with GE only. If the coleoptile segments were first preincubated with GE and subsequently GE was removed, the addition of IAA induced strong growth and medium acidification. Dithiothreitol added together with GE neutralized the toxic effect of GE on growth of coleoptile segments incubated in the presence of IAA. The addition of GE to the control medium caused a depolarization of the membrane potential, the value of witch depended on GE dilution. These results indicate that the toxic effect of GE on growth of plant cells might be caused by disruption of the catalytic function of the plasma membrane H ? -ATPase on formation of the disulfide bonds.

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“…The urease inhibitors such as hydroxamic acids and phosphoroamides have been applied in therapy for blocking the enzyme action [10]. The inhibitory ability also shows the garlic extract applied as a natural antibiotic against urease [11].…”

Section: Introductionmentioning

confidence: 99%

Kinetics of jack bean urease inhibition by 2,3-dichloro-1,4-naphthoquinone. Elucidation of the mechanism: redox cycling and sulfhydryl arylation

Zaborska

Kot

Bala

2009

Journal of Enzyme Inhibition and Medicinal Chemistry

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A study of the inhibition of jack bean urease by garlic extract

Cited by 43 publications

References 24 publications

In vitroscreening and evaluation of some Indian medicinal plants for their potential to inhibit Jack bean and bacterial ureases causing urinary infections

In vitroscreening and evaluation of some Indian medicinal plants for their potential to inhibit Jack bean and bacterial ureases causing urinary infections

Effect of thiosulphinates contained in garlic extract on growth, proton fluxes and membrane potential in maize (Zea mays L.) coleoptile segments

Kinetics of jack bean urease inhibition by 2,3-dichloro-1,4-naphthoquinone. Elucidation of the mechanism: redox cycling and sulfhydryl arylation

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