2016
DOI: 10.3892/ol.2016.4087
|View full text |Cite
|
Sign up to set email alerts
|

A study of the potential anticancer activity of Mangifera zeylanica bark: Evaluation of cytotoxic and apoptotic effects of the hexane extract and bioassay-guided fractionation to identify phytochemical constituents

Abstract: The present study investigated the potential anticancer activity of the bark of Mangifera zeylanica, an endemic plant in Sri Lanka that has been traditionally used for cancer therapy. Cytotoxic and apoptotic effects were investigated in vitro using sulphorodamine assay, acridine orange and ethidium bromide staining, caspase-3 and −7 activity, DNA fragmentation and reverse transcription-quantitative polymerase chain reaction in estrogen receptor positive MCF-7 and triple-negative MDA-MB-231 breast cancer cell l… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
36
0

Year Published

2018
2018
2023
2023

Publication Types

Select...
4
1

Relationship

2
3

Authors

Journals

citations
Cited by 35 publications
(36 citation statements)
references
References 27 publications
0
36
0
Order By: Relevance
“…The dye SRB can bind with proteins in viable cells under mild acidic conditions, and the bound dye is finally solubilized to get absorbance at 500–540 nm (Skehan et al, ). The SRB and MTT assays have been extensively used to assess cytotoxic effects of pure compounds and extracts in our laboratory (Ediriweera, Tennekoon, Adhikari, et al, ; Ediriweera et al, ; Ediriweera et al, ; Ediriweera, Tennekoon, Samarakoon, Thabrew, & De Silva, ; Ediriweera, Tennekoon, Samarakoon, Thabrew, & de Silva, ; Ediriweera, Tennekoon, Samarakoon, Thabrew, & Dilip De Silva, ; Jayarathna et al, ; Nwokwu et al, ; Tharmarajah et al, ). Although these assays (MTT, MTS, XTT and WST) are cheap and easy to conduct, interaction of testing substances with the salts/dyes and damaging of cells during washing steps are the major disadvantages of these assays (Wang, Henning, & Heber, ).…”
Section: Cell Viability/cytotoxicity and Antiproliferative Assaysmentioning
confidence: 99%
See 4 more Smart Citations
“…The dye SRB can bind with proteins in viable cells under mild acidic conditions, and the bound dye is finally solubilized to get absorbance at 500–540 nm (Skehan et al, ). The SRB and MTT assays have been extensively used to assess cytotoxic effects of pure compounds and extracts in our laboratory (Ediriweera, Tennekoon, Adhikari, et al, ; Ediriweera et al, ; Ediriweera et al, ; Ediriweera, Tennekoon, Samarakoon, Thabrew, & De Silva, ; Ediriweera, Tennekoon, Samarakoon, Thabrew, & de Silva, ; Ediriweera, Tennekoon, Samarakoon, Thabrew, & Dilip De Silva, ; Jayarathna et al, ; Nwokwu et al, ; Tharmarajah et al, ). Although these assays (MTT, MTS, XTT and WST) are cheap and easy to conduct, interaction of testing substances with the salts/dyes and damaging of cells during washing steps are the major disadvantages of these assays (Wang, Henning, & Heber, ).…”
Section: Cell Viability/cytotoxicity and Antiproliferative Assaysmentioning
confidence: 99%
“…Therefore, before conducting experiments, particularly with multiple fluorescent probes, the user must optimize spatial resolution and have an idea about excitation and emission spectra of each probe (Combs, ; Liu et al, ; Pringle et al, ; Renz, ; Stockert & Blazquez‐Castro, ). The fluorescent microscopic technique has been used to detect morphological changes upon treatment with several phytochemicals and crude drugs in various cancer cells (breast, ovarian, lung and liver) in studies conducted in our laboratory (Ediriweera et al, ; Ediriweera, Tennekoon, Samarakoon, et al, ; Samarakoon et al, ). Two previously reported studies (Ali et al, ; Nagappan et al, ) cited under Section have also used fluorescence microscopy to observe apoptotic changes in cancer cells.…”
Section: Detection Of Apoptosismentioning
confidence: 99%
See 3 more Smart Citations