A study of the role of multiple layer‐by‐layer assembled bionic extracellular matrix in promoting wound healing via activation of the Wnt signaling pathway

Abstract

Wound healing is a multicellular collaborative process in which the adhesion and proliferation of fibroblasts in the wound is the basis for ensuring rapid wound healing, and in this process, it can promote the regeneration and remodeling of tissue and extracellular matrix. Studies have shown that Arg-Gly-Asp adhesive peptide (RGD) and basic fibroblast growth factor (bFGF) can stimulate the adhesion and proliferation of fibroblasts by activating the Wnt/β-catenin signaling pathway, respectively. This study adopts the principle of layer-by-layer self-assembly, and the binding force formed by electrostatic attraction and Schiff base was used to combine bFGF and RGD with collagen membrane to form a biomimetic membrane that is non-cytotoxic with strong biocompatibility that could promote soft tissue healing. The surface characteristics of MLCM and the sustained release concentration of bFGF in vitro were measured, and the effects of MLCM on cell viability, proliferation, migration, and wound healing by means of Wnt/catenin pathways were studied through cell experiments and animal experiments under the comparison of negative control groups and positive control groups. The results showed that MLCM could stimulate wound healing more actively and had a positive effect on cell activity, proliferation, and migration. During wound healing, MLCM activates the Wnt/β-catenin signaling pathway and inhibition of Wnt/β-catenin signaling pathway significantly reduces the positive effects of MLCM on wound healing.

Scaffold Adhering to Peptide-Based Biomimetic Extracellular Matrix Composite Nanobioglass Promotes the Proliferation and Migration of Skin Fibroblasts Through the GSK-3β/β-Catenin Signaling Axis

“…It also indicated that the collage, along with adhesion peptides and cytokines, which provide tissue support during the healing process, can be the influential factors in the proliferation of skin fibroblasts. 26 These findings further supported the potential clinical applications of BM in wound healing at the cellular level.…”

“…Additionally, our research group has previously shown that biomimetic extracellular matrix collagen membranes containing BFGF and RGD can enhance the adhesion and proliferation of skin fibroblasts. 26 …”

"Simmer pus and grow flesh" method promotes chronic wound healing in rats via bFGF-Wnt β-catenin signaling pathway

“…At the molecular level, the expressions of bFGF, GSK-3β and c-Myc protein in wound granulation tissue in model group were higher than control group, while the expression of β-catenin was lower. This indicated that chronic infectious wounds may lead to abnormal activation of Wnt/β-catenin signaling pathway and affect the healing process of wounds [19]. However, the expressions of bFGF, GSK-3β and c-Myc protein in experimental group and positive control group were lower than model group, while the expression of β-catenin was significantly higher.…”

“…At the same time, bFGF may regulate Wnt/β-catenin signal pathway, promote angiogenesis and improve blood supply of wound surface, which is beneficial to nutrient supply and oxygen delivery during wound healing. In addition, bFGF regulating Wnt/β-catenin signaling pathway may adjust inflammatory reactions, reduce the degree of wound inflammation and contribute to the smooth progress of wound healing [18,19]. In addition, the method of simmering pus and growing meat is a traditional Chinese drug treatment method, which is widely used in chronic wound healing.…”

The effect and mechanism of selenium supplementation on the proliferation capacity of bovine endometrial epithelial cells exposed to lipopolysaccharide in vitro under high cortisol background

“…The PI3K/AKT and Wnt/β-catenin signaling pathways have been shown to be closely related to cell proliferation ( Hennessy et al, 2005 ; Wang et al, 2023 ). When both signaling pathways are activated, BEECs proliferate to promote endometrial repair ( Dong et al, 2019 ).β-catenin is the central signaling protein of the Wnt/β-catenin signaling pathway.…”