A study of the THP‐1 cell line as the potential biologics production platform with the emphasis on serum‐free media substitution for economic expediency

Abstract

Cell cultures are frequently preferred in the industrial production of high value-added biopharmaceuticals on a large scale. In the production of biopharmaceuticals, the selection of the appropriate cell line, the cell culture medium, and the culture conditions are very important. In medical products to be offered for human use, authorized institutions do not allow the use of serum which increases the culture yield, added to the culture. For this reason, the cell lines to be used must be adapted to the serumfree medium. In this study, first, the direct and gradual adaptation of the THP-1 cell line, which has a high potential for use in biopharmaceutical production, to a locally produced chemically defined serum-free medium was carried out. Then, a comparison of the production efficiency in the shake flasks with the commercially available two different serum-free media was performed. Finally, for the first time, THP-1 cells were produced in spinner flasks and stirring tank bioreactor to simulate the large scale within the scope of this work.

Animal Cell Lines as Expression Platforms in Viral Vaccine Production: A Post Covid-19 Perspective

“…In this sense, those with the lowest risk are epithelial and fibroblastic cells, while the cells with the highest risk are cells of hematopoietic origin. However, when risk assessment is made with advancing molecular techniques, all cell lines of different origins have the potential to be used in commercial production as expression platforms when their safety is proven. ,,, In this respect, cell lines that are not currently used as expression platforms in the biotechnology industry but have high potential in this regard should be evaluated. − …”

“… 10 , 12 , 95 , 101 In this respect, cell lines that are not currently used as expression platforms in the biotechnology industry but have high potential in this regard should be evaluated. 102 − 104 …”

Suspension culture promoted the expansion of NK‐92 cells ex vivo by enhancing the expression of IL‐2 receptor

“…Suspension culture using a bioreactor is an efficient way for large‐scale production of immunocyte and chimeric antigen receptor‐engineered cells 20,21 . Compared with static culture, suspension culture based on bioreactor is easy to enlarge and monitor, and has the advantage of homogenizing medium, which is beneficial to the diffusion of substances and promotes the uptake of nutrients by cells 22–24 . However, the force caused by fluid mixing in the bioreactor can affect gene and protein expression, intracellular signaling pathways, and plasma membrane permeability 25 .…”

“…20,21 Compared with static culture, suspension culture based on bioreactor is easy to enlarge and monitor, and has the advantage of homogenizing medium, which is beneficial to the diffusion of substances and promotes the uptake of nutrients by cells. [22][23][24] However, the force caused by fluid mixing in the bioreactor can affect gene and protein expression, intracellular signaling pathways, and plasma membrane permeability. 25 Therefore, suspension culture in bioreactor is the trend in the large-scale production of NK cells, but little is known about what effect suspension culture will have on the IL-2 receptor expression and signal transduction of NK cells.…”