A study of the xenoantigenicity of adult pig islets cells

Abstract

The origin of antigenicity of pig islets is mainly N-linked sugars including sialic acid antigens, but not the alpha-Gal, and pig islets can be injured by both the classical and the alternative complement pathway in human serum.

Mass spectrometric analysis of the glycosphingolipid‐derived glycans from miniature pig endothelial cells and islets: identification of NeuGc epitope in pig islets

“…[32,33] Interestingly, 13 of the NeuGc-terminated N-glycans from pig islets that could activate the human complement immune pathway were first detected. [34] Moreover, the existence of NeuGc in GSL-derived glycans from pig islets was initially demonstrated using mass spectrometry in this study. This is important in NeuGc immunogenicity research for pig islet xenotransplantation.…”

Immunological Property of Antibodies against N-Glycolylneuraminic Acid Epitopes in Cytidine Monophospho–N-Acetylneuraminic Acid Hydroxylase-Deficient Mice

“…In a previous study, the expression of NeuGc epitopes on islets from adult or neonatal pigs was clearly detected, and the antigenicity of adult pig islets or neonatal porcine islet-like cell clusters was found to be mainly associated with N-linked sugars, including NeuGc epitopes, but not Gal Ags (41)(42)(43)(44). These findings led us to investigate the immunogenicity of NeuGc epitopes located on the islets by using a CMAH +/+ -to-CMAH 2/2 mouse islet transplantation model.…”

A lectin microarray study of glycoantigens in neonatal porcine islet-like cell clusters

“…Two types of islet transplantations are under consideration. One is the transplantation of adult pig islets (APIs) [7]. The other is the transplantation of neonatal porcine islet-like cell clusters (NPCCs) [8].…”