Introduction: After the first laparoscopic cholecystectomy was performed in 1987, the 4-port operation technique became the standard worldwide. This study is a comparison of the intraoperative complications observed using 3-port and 4-port methods, and an examination of the reliability of the 3-port method. Materials and Methods: The files of 400 patients who underwent a laparoscopic cholecystectomy due to gallbladder disease between 2014 and 2019 were analyzed. The patients were divided into 2 groups according to the use of a 3-port or a 4-port method, and the intraoperative complications of the groups were compared. Results: A total of 400 patient files were reviewed and the only significant difference between the 2 groups among the parameters of age, gender, and weight was the female-male ratio. Of the 3-port cases, 13.5% were male, and 86.5% were female. Of the 4-port cases, 23.5% were male and 76.5% were female (p=0.010). A significant difference was not seen between the 2 groups with respect to the mean operation time (p=0.548) or operation success (p=0.253). The average hospitalization period was longer in the 3-port group (1.98 days vs 1.18 days, respectively; p<0.001). No significant difference was found in the number of intraoperative complications: gallbladder perforation (p=0.215), liver laver hemorrhage (p=0.481), entry of the gallstone into the abdomen (p=0.760), and choledoch injury (p=0.522). Conclusion: The success rate of a laparoscopic cholecystectomy was similar in the 3-port and 4-port groups. The mean length of hospitalization was longer in the 3-port group compared with the 4-port group. Analysis suggested that the greater number of intraoperative complications in the 3-port group had an effect on the hospitalization period. There were no instances of further complications developing after the 3-port procedure.