A Study on Dual-Response Composite Hydrogels Based on Oriented Nanocellulose

Dong, Lei; Liang, Mujiao; Guo, Zhongwei; Wang, Anyang; Cai, Gangpei; Yuan, Tianying; Mi, Shengli; Sun, Wei

doi:10.18063/ijb.v8i3.578

Cited by 5 publications

(13 citation statements)

References 45 publications

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“…35,36,43 More recently, printed composites composed of cellulose fibrils and hyaluronic acid methacrylate have also been explored from organizing fibroblasts. 44 Similarly, previous anisotropic soft substrates for muscle-printed cNFC:gelatin enable the maturation of human myotubes for at least several weeks. The myotubes displayed a steady increase in size and basic metabolic function, indicating that cNFC:gelatin composites were not cytotoxic.…”

Section: ■ Discussionmentioning

confidence: 97%

“…Soft and structured hydrogel substrates have previously been shown beneficial for self-organization, long-term culturing, and maturation of engineered striated muscle tissues. − , Similarly, synthetic nanofiber scaffolds produced, e.g., by force-spinning or electrospinning are efficient for generating anisotropic engineered muscle tissues. ,, More recently, printed composites composed of cellulose fibrils and hyaluronic acid methacrylate have also been explored from organizing fibroblasts . Similarly, previous anisotropic soft substrates for muscle-printed cNFC:gelatin enable the maturation of human myotubes for at least several weeks.…”

Section: Discussionmentioning

confidence: 99%

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Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks

Radeke

Pons

Mihajlovic

et al. 2023

ACS Appl. Mater. Interfaces

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For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissuemimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties. Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities. Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation. This way, we generate partially negatively charged cellulose nanofibers with diameters of ∼250 nm and lengths spanning tens to hundreds of microns. In this range, the fibers structurally match the size and dimensions of natural collagen fibers making them sufficiently large to orient cells. Yet, they are simultaneously sufficiently thin to be optically transparent. By adjusting fiber concentration, 3D printing inks with excellent shear-thinning properties can be established. In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)derived proteins can easily be generated. We apply such composite inks for 3D printing cell-laden and cross-linkable structures, as well as tissue-guiding gel substrates. Interestingly, we find that the spatial organization of engineered tissues can be defined by the shear-induced alignment of fibers during the printing procedure. Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents. Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.

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Section: ■ Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks

Radeke

Pons

Mihajlovic

et al. 2023

ACS Appl. Mater. Interfaces

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“…Composite hydrogels with different compositions were obtained by combining three components, RHCMA, HAMA, and AgNCs, as shown in Table . After full dissolution, the resulting mixtures were irradiated by UV light (wavelength: 365 nm, light intensity: 10 mW cm –2 , and time: 2 min) with 1% (w/v) Irgacure 2959 to induce the formation of a covalent crosslinking network between RHCMA and HAMA . Before UV crosslinking, the materials were called hydrogel precursors.…”

Section: Methodsmentioning

confidence: 99%

“…The hydrogel precursors were loaded in a 3 mL syringe that was fixed to the extrusion printhead of a 3D printer (SunP CPD1/BioMaker). BioMaker software was used to design the printing model and set the printing parameters . A 25 G needle was used during the process.…”

Section: Methodsmentioning

confidence: 99%

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Collagen–Hyaluronic Acid Composite Hydrogels with Applications for Chronic Diabetic Wound Repair

Liang,

Dong,

Guo

et al. 2023

ACS Biomater. Sci. Eng.

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Chronic diabetic wounds have become a major healthcare challenge worldwide. Improper treatment may lead to serious complications. Current treatment methods including biological and physical methods and skin grafting have limitations and disadvantages, such as poor efficacy, inconvenience of use, and high cost. Therefore, developing a more effective and feasible treatment is of great significance for the repair of chronic diabetic wounds. Hydrogels can be designed to serve multiple functions to promote the repair of chronic diabetic wounds. Furthermore, 3D bioprinting enables hydrogel customization to fit chronic diabetic wounds, thus facilitating the healing process. This paper reports a study of 3D printing of a collagen−hyaluronic acid composite hydrogels with application for chronic diabetic wound repair. In situ printed hydrogels were developed by a macromolecular crosslinking network using methacrylated recombinant human collagen (RHCMA) and methacrylated hyaluronic acid (HAMA), both of which can respond to ultraviolet (UV) irradiation. The hydrogels were also loaded with silver nanoclusters (AgNCs) with ultra-small-size nanoparticles, which have the advantages of deep penetration ability and broad-spectrum high-efficiency antibacterial properties. The results of this study show that the developed RHCMA, HAMA, and AgNCs (RHAg) composite hydrogels present good UV responsiveness, porosity, mechanical properties, printability, and biocompatibility, all of which are beneficial to wound healing. The results of this study further show that the developed RHAg hydrogels not only effectively inhibited Staphylococcus aureus and Pseudomonas aeruginosa but also promoted the proliferation and migration of fibroblasts in vitro and tissue regeneration and collagen deposition in vivo, thus producing a desirable wound repair effect and can be used as an effective functional biomaterial to promote chronic diabetic wound repair.

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