Doctors or health laboratory workers usually carry out manual blood examinations, who cell morphology parameters by eye. Electrical impedance spectroscopy has been proposed to assist doctors in examining blood damage. The effect of storage on blood cells will be evaluated using an electrical impedance measurement system on blood cells. The results of electrical impedance measurements will be correlated with the quality and quantity of blood cells, namely the mean corpuscular hemoglobin concentration (MCHC) value. Three bags of complete blood cell packages (from different donors) and 1 bag of red blood cell packages were used as blood samples. All blood samples using citrate phosphate dextrose adenine (CPDA) anticoagulant were obtained from the Indonesian Red Cross, Malang City Branch. Electrical impedance and cell-count measurements were performed on Day 0 (before being put into the refrigerator) and on Days 2, 4, 7, 10, 17, 21 and 28 after being placed in the fridge. Electrical impedance was measured using Bio-Impedance Spectroscopic Data Acquisition (BISDAQ). As the duration of storage increases, the MCHC value tends to decrease. The total impedance tends to decrease with increasing storage duration; the total impedance on Day 28 shows the lowest value. In general, the resistance value of the solution is correlated with the MCHC value of the blood cells. The escape of some material in the cell to the solution outside the cell results in a decrease in the resistance properties of the solution, according to the study’s results. The total electrical impedance decreases with increasing storage duration. Storage duration affects the quality and quantity of blood cells. The MCHC value of blood cells decreased with increasing storage duration. The value of MCHC is strongly correlated with the value of the external solution resistance of the cell.
HIGHLIGHTS
The total electrical impedance decreases with increasing storage duration
Storage duration affects the quality and quantity of blood cells
The MCHC value is strongly correlated with the value of the external solution resistance of the cell. The decrease in the MCHC value results in a reduction in the value of the external solution resistance of the cell
GRAPHICAL ABSTRACT