A Study on Methicillin Resistant Staphylococcus aureus Phage Types in Wound Infections and its Antibiotic Susceptibility Pattern at King George Hospital, Visakhapatnam, India

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) is a growing concern in the community and in hospitals ever since it has been diagnosed. As staphylococcus has been the dominant pathogen of wounds, Methicillin resistance made the treatment a tough challenge to the clinician. The growing problem of antibiotic resistance and the need for alternative treatments have led to a reappearance of importance in phage therapy. Scope: The present study aimed on MRSA phage types and its antibiotic susceptibility pattern in wounds. Material and Methods: In this study a total of 92 coagulase positive Staphylococcus aureus obtained from various wounds are identified by inoculating on Blood agar, MacConkey agar and Nutrient agar and processed further by necessary biochemical tests and tube coagulase tests. All strains were tested for Methicillin resistance and phage typing. Findings: The results obtained were MRSA (22) of 23.92%, Methicillin sensitive staphylococcus aureus (MSSA) (70) of 76.08% and the predominant phage type in MRSA and MSSA obtained was phage III. The typable MRSA strains and MSSA strains showed 100% sensitivity to Vancomycin followed by 89.13% sensitivity to Cefaperazone-Sulbactum, 87.5% to Ciprofloxacin. Conclusion: Phage typing may be a very useful diagnostic method in future.