A Study on Proliferation and Behavior of Retinal Pigment Epithelial Cells on Purified Alginate Films

Abstract

Background and Objectives: Alginate, an anionic polysaccharide distributed widely in the cell walls of brown algae, is used in biomedical applications. However, alginate's performance as a biomaterial, has limited by its several contamination such as endotoxins, proteins and polyphenols. Methods and Results: To overcome this problem, we have developed using modified Korbutt method for alginate purification. After purification, we made alginate films and used for retinal pigment epithelial cell (RPEs) regeneration. ARPE-19 cells were seeded in non-purified and purified alginate films, and then cell viability and proliferation were estimated by MTT assay and RT-PCR was performed to assess specific cell expression. ARPE-19 cell-loaded alginate films were evaluated specific protein expression by through AEC staining and we examined the cell adhesion by scanning electron micro scopy (SEM). Conclusions: In this result, ARPE-19 cells in purified alginate films had higher cell proliferative rate and phenotypic expression than those on non-purified alginate films. The results suggest that purified alginate is useful for RPEs regeneration.

Effect of pore sizes of PLGA scaffolds on mechanical properties and cell behaviour for nucleus pulposus regenerationin vivo

“…Before testing for cell viability, each sample was observed to determine cell migration within the porous structure of the scaffold; then each sample of 100 µl was pipetted into a 96‐well plate and the absorbance at 570 nm, using an ELISA microplate reader (E‐Max, Molecular Devices, Sunnyvale, CA, USA), was yielded as a function of viable cell number. The rate of tetrazolium (MTT) reduction is proportional to the rate of cell viability (Jeong et al ., ).…”

Effects of small intestinal submucosa content on the adhesion and proliferation of retinal pigment epithelial cells on SIS-PLGA films

“…The decellularized SIS was washed with saline solution, followed by 0.1% peracetic acid, and sliced at about 7 cm in the longitudinal direction. The slices were freeze‐dried using a freeze dryer (model FDU‐540, EYELA, Japan) at –80°C for 48 h, and the dried SIS was obtained using a cryogenic laboratory mill (SPEX 6750 Freezer Mill, SPEX, NJ, USA) at –198°C to yield a SIS powder of particle size < 180 µm (Kim et al ., ; Oh et al ., ; Yoshioka et al ., ; Giordano et al ., ; Jeong et al ., ; Jang et al ., ; Song et al ., ).…”

“…AMD) or by surgical excision of CNV may maintain photoreceptor function in patients (Itaya et al ., ). There exist methods of transplantation of RPE cells; one is RPE suspensions (Radtke et al ., ; Aramant et al ., ; Woch et al ., ), which form a monolayer when transplanted into the subretinal space; another is cultivating RPE cells on a scaffold (Jeong et al ., ). However, using the former method to transplant RPE cells is often accompanied by significant cell loss, migration and fibrosis at the subretinal space after transplantation (Lopez et al ., ; Kubota et al ., 2006).…”

Retinal cell regeneration using tissue engineered polymeric scaffolds

“…Hunt et al further demonstrated the potential of RGD-alginate scaffolds for the derivation, transport, and transplantation of neural retina and RPE [44]. Purified alginate films have also been manufactured and used as a scaffold of for RPE cells [74]. This study indicated that purified alginate films allowed higher cell proliferative rate and phenotypic expression compared with nonpurified alginate films.…”