A study on silicosis among employees of a silica processing factory in the Central Province of Sri Lanka

Siribaddana, Anoma; Wickramasekera, K; Palipana, W M; Peiris, M. D. P.; Upul, B K A; Senevirathna, Kapila; Dassanayake, Dinesh

doi:10.4038/cmj.v61i1.8252

Cited by 11 publications

(13 citation statements)

References 3 publications

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“…Evidence has been presented that silica dust acute and chronic exposures will trigger an inflammatory cascade, such as macrophages and alveolar epithelial cells proliferated successively, followed by inflammatory cascade; the fibrotic development will be ignited, which eventually will proceed to determine the silicosis [1, 2]. Silicosis is an irreversible and incurable pulmonary disorder; even when patients are no longer exposed to silica, the fibrosis remains progressive [3, 4]. Silica dust is widely distributed in workplaces in which drilling, grinding, and hammering activities occur [5, 6].…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of lncRNA and mRNA Transcriptomes Reveals New Regulators of Ubiquitination and the Immune Response in Silica-Induced Pulmonary Fibrosis

Zhou

Liu

et al. 2019

BioMed Research International

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Objectives As an epigenetic player, long noncoding RNAs (LncRNAs) have been reported to participate in multiple biological processes; however, their biological functions in silica-induced pulmonary fibrosis (SIPF) occurrence and development remain incompletely understood. Methods Five case/control pairs were used to perform integrated transcriptomes analysis of lncRNA and mRNA. Prediction of lncRNA and mRNA functions was aided by the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Additionally, we constructed a coexpression network of lncRNAs and mRNAs to identify targets of regulation. Results In total, 1069 differentially expressed mRNAs and 366 lncRNAs were identified with the changes more than 2 times (p<0.05), of which 351 downregulated mRNA and 31 downregulated lncRNA were <0.5 (p<0.05) and those of 718 upregulated mRNAs and 335 upregulated lncRNA were >2 (p<0.05). The levels of 10 lncRNAs were measured via qRT-PCR; the results were consistent with the microarray data. Four genes named of FEM1B, TRIM39, TRIM32, and KLHL15 were enriched significantly with ubiquitination and immune response. Cytokine-cytokine receptor interaction was the most significantly enriched KEGG pathway in both mRNAs and lncRNAs. The coexpression network revealed that a single lncRNA can interact with multiple mRNAs, and vice versa. Conclusions lncRNA and mRNA expression were aberrant in patients with SIPF compared to controls, indicating that differentially expressed lncRNAs and mRNAs may play critical roles in SIPF development. Our study affords new insights into the molecular mechanisms of SIPF and identifies potential biomarkers and targets for SIPF diagnosis and treatment.

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Section: Introductionmentioning

confidence: 99%

Integrated Analysis of lncRNA and mRNA Transcriptomes Reveals New Regulators of Ubiquitination and the Immune Response in Silica-Induced Pulmonary Fibrosis

Zhou

Liu

et al. 2019

BioMed Research International

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“…Oxidative agents produced by silica particles facilitate the release of cytokines and the mobilization of neutrophils and macrophages to the injured sites. With inflammatory cells constantly infiltrating the lungs, more ROS are generated, eventually resulting in the inflammation and fibrosis in the lungs (Siribaddana et al, ). High‐throughput microarray detection is one of the essential tools to study microRNA expression profiles.…”

Section: Introductionmentioning

confidence: 99%

Down‐Regulation of miR‐19a as a Biomarker for Early Detection of Silicosis

Yang

Li²,

Yao

et al. 2016

The Anatomical Record

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The accumulated data indicate that there is significant genetic heterogeneity underlying the etiology of silicosis. Recent reports have revealed that microRNAs (miRNAs) play an important role in regulating pulmonary fibrosis. This study, therefore, aimed to identify some miRNAs as biomarkers for silicosis, and to explore the early diagnostic value of biomarkers for silicosis. Total RNAs were collected from the peripheral blood leukocytes of 23 silicosis patients and 23 healthy controls, the different miRNAs were screened using microarrays. The potential biomarker miRNAs were identified by quantitative real-time polymerase chain reaction (qPCR) and receiver operating characteristic (ROC) curves. Eighteen differential miRNAs in leukocytes were up-regulated and twenty differential miRNAs were down-regulated in the silicosis group, compared with the control group. The expression levels of miR-181a and miR-19a were 0.8854 6 0.1037 and 0.2929 6 0.0342 by the relative quantitation method 2 2᭝᭝CT of qPCR, respectively. The sensitivity and specificity for miR-181a at a cut-off value of 1.8917 were 70% and 75%, respectively, whereas, those for miR-19a at a cut-off value of 3.6828 were 95% and 95%, respectively. Thus, miR-19a in peripheral blood leukocyte could be used as an effective biomarker for silicosis. Anat Rec, 299:1300Rec, 299: -1307Rec, 299: , 2016. V C 2016 Wiley Periodicals, Inc. Key words: silicosis; miR-19a; biomarkerAbbreviations used: AUC 5 the area under the ROC curve; EDTA 5 ethylene diamine tetraacetic acid; IPF 5 idiopathic pulmonary fibrosis; qPCR 5 quantitative real-time PCR; ROC 5 receiver operating characteristic; ROS 5 reactive oxygen species; RIN 5 RNA integrity number; TNF 5 tumor necrosis factor; TF 5 tissue factor.

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“…Ongoing studies continue to support the increased sensitivity and specificity of HRCT over chest radiography for changes related to occupational lung disease [26,43,[56][57][58], although the level of radiologist expertise can affect interpretation [59]. HRCT proves central in the imaging of classic and emerging pneumoconioses [6,34,[60][61][62][63][64], as well as differentiating occupational lung disease from other ILDs [65,66]. New HRCT findings are revealing additional imaging characteristics important to the diagnosis of occupational lung disease [67][68][69][70].…”

Section: Discussion Of Procedures By Variantmentioning

confidence: 99%

ACR Appropriateness Criteria® Occupational Lung Diseases

Imaging¹,

Cox

Chung

et al. 2020

Journal of the American College of Radiology

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A study on silicosis among employees of a silica processing factory in the Central Province of Sri Lanka

Abstract: Objectives The silica industry is rapidly expanding in Sri Lanka. The main objective of this study was to assess the prevalence of silicosis among workers exposed to silica dust, and to describe the disease pattern of the affected. We also screened the affected workers for tuberculosis.

Cited by 11 publications

References 3 publications

Integrated Analysis of lncRNA and mRNA Transcriptomes Reveals New Regulators of Ubiquitination and the Immune Response in Silica-Induced Pulmonary Fibrosis

Integrated Analysis of lncRNA and mRNA Transcriptomes Reveals New Regulators of Ubiquitination and the Immune Response in Silica-Induced Pulmonary Fibrosis

Down‐Regulation of miR‐19a as a Biomarker for Early Detection of Silicosis

ACR Appropriateness Criteria® Occupational Lung Diseases

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