A study on surface endothelialization of plasma coated intravascular stents

Tang, Chaojun; Wang, Guixue; Shen, Yang; Wan, Li‐Jun; Li, Xiao; Zhang, Q.; Yu, Qingsong; Liu, L.S.; Wen, Ge

doi:10.1016/j.surfcoat.2009.09.084

Cited by 17 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They need to be further modified to reduce their hemolytic activity and improve their biocompatibility. So the proper surface modifications, such as plasma surface deposition [37], endothelial cell seeding [38], micropatterned surface modification [39], composite membrane coating [40,41], drug eluting coating [42] etc., should be adopted to improve their hemocompatibility [21,43,44].…”

Section: Discussionmentioning

confidence: 99%

Study on the biodegradability and biocompatibility of WE magnesium alloys

Wang

Shen

et al. 2012

Materials Science and Engineering: C

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Study on the biodegradability and biocompatibility of WE magnesium alloys

Wang

Shen

et al. 2012

Materials Science and Engineering: C

View full text Add to dashboard Cite

“…A bell jar-type plasma reactor system was used in this study, which was described previously in our recent research [20]. Before plasma deposition, the wafers were pretreated and cleaned with oxygen plasma for 2 min under conditions of 2 sccm oxygen flow, 25 mtorr pressure and 5 W DC power input.…”

Section: Plasma Coating and Surface Characterizationmentioning

confidence: 99%

Surface wettability of plasma SiO _x :H nanocoating-induced endothelial cells' migration and the associated FAK-Rho GTPases signalling pathways

Shen

Wang

Huang

et al. 2011

J. R. Soc. Interface.

View full text Add to dashboard Cite

Vascular endothelial cell (EC) adhesion and migration are essential processes in re-endothelialization of implanted biomaterials. There is no clear relationship and mechanism between EC adhesion and migration behaviour on surfaces with varying wettabilities. As model substrates, plasma SiO x :H nanocoatings with well-controlled surface wettability (with water contact angles in the range of 98.5 + 2.38 to 26.3 + 4.08) were used in this study to investigate the effects of surface wettability on cell adhesion/migration and associated protein expressions in FAK-Rho GTPases signalling pathways. It was found that EC adhesion/migration showed opposite behaviour on the hydrophilic and hydrophobic surfaces (i.e. hydrophobic surfaces promoted EC migration but were anti-adhesions). The number of adherent ECs showed a maximum on hydrophilic surfaces, while cells adhered to hydrophobic surfaces exhibited a tendency for cell migration. The focal adhesion kinase (FAK) inhibitor targeting the Y-397 site of FAK could significantly inhibit cell adhesion/migration, suggesting that EC adhesion and migration on surfaces with different wettabilities involve ( p)FAK and its downstream signalling pathways. Western blot results suggested that the FAK-Rho GTPases signalling pathways were correlative to EC migration on hydrophobic plasma SiO x :H surfaces, but uncertain to hydrophilic surfaces. This work demonstrated that surface wettability could induce cellular behaviours that were associated with different cellular signalling events.

show abstract

“…Materials with biomolecular or topographical modifications that mimic natural biological tissue hierarchy may provide solutions to the problem of biomaterial rejection. [4][5][6][7][8][9] Previous research has evaluated the biocompatibility of various biomaterials through an in-depth look into cellular adhesion and proliferation, 10,11 cell phenotypic behavior, 12,13 material cytotoxic effects, 14,15 and protein folding and interactions on material surfaces. 16,17 Several studies have shown increased cellular functionality on peptide-modified [18][19][20][21] and growth-factor functionalized surfaces.…”

Section: Introductionmentioning

confidence: 99%

Hemocompatibility of titania nanotube arrays

Smith

Yoriya

Grissom³

et al. 2010

J Biomedical Materials Res

114

View full text Add to dashboard Cite

Hemocompatibility is a key consideration for the long-term success of blood contacting biomaterials; hence, there is a critical need to understand the physiological response elicited from blood/nano-biomaterial interactions. In this study, we have investigated the adsorption of key blood serum proteins, in vitro adhesion and activation of platelets, and clotting kinetics of whole blood on titania nanotube arrays. Previous studies have demonstrated improved mesenchymal stem cell functionality, osteoblast phenotypic behavior, localized drug delivery, and the production of endothelial cell ECM on titania nanotube arrays. Furthermore, these titania nanotube arrays have elicited minimal levels of monocyte activation and cytokine secretion, thus exhibiting a very low degree of immunogenicity. Titania nanotube arrays were fabricated using anodization technique and the surface morphology was examined through scanning electron microscopy (SEM). The crystalline phases were identified using glancing angled X-ray diffraction (GAXRD). Nanoindentation and scratch tests were used to characterize the mechanical properties of titania nanotube arrays. The adsorption of key blood proteins (albumin, fibrinogen, and immunoglobulin-g) was evaluated using a micro-BCA assay and X-ray photoelectron spectroscopy (XPS). The adhesion and activation of platelets was investigated using live-cell staining, MTT assay, and SEM. Whole blood clotting kinetics was evaluated by measuring the free hemoglobin concentration, and SEM was used to visualize the clot formation. Our results indicate increased blood serum protein adsorption, platelet adhesion and activation, and whole blood clotting kinetics on titania nanotube arrays.

show abstract

A study on surface endothelialization of plasma coated intravascular stents

Cited by 17 publications

References 31 publications

Study on the biodegradability and biocompatibility of WE magnesium alloys

Study on the biodegradability and biocompatibility of WE magnesium alloys

Surface wettability of plasma SiO _x :H nanocoating-induced endothelial cells' migration and the associated FAK-Rho GTPases signalling pathways

Hemocompatibility of titania nanotube arrays

Contact Info

Product

Resources

About

A study on surface endothelialization of plasma coated intravascular stents

Cited by 17 publications

References 31 publications

Study on the biodegradability and biocompatibility of WE magnesium alloys

Study on the biodegradability and biocompatibility of WE magnesium alloys

Surface wettability of plasma SiO x :H nanocoating-induced endothelial cells' migration and the associated FAK-Rho GTPases signalling pathways

Hemocompatibility of titania nanotube arrays

Contact Info

Product

Resources

About

Surface wettability of plasma SiO _x :H nanocoating-induced endothelial cells' migration and the associated FAK-Rho GTPases signalling pathways