A Study on the Acoustic Response of Pickering Perfluoropentane Droplets in Different Media

Loskutova, Ksenia; Nimander, Didrik; Gouwy, Isabelle; Chen, Hongjian; Ghorbani, Morteza; Svagan, Anna J.; Grishenkov, Dmitry

doi:10.1021/acsomega.0c06115

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…CNFs have many hydroxyl groups present at the surface, which makes it easy to functionalize the droplet shell to target specific cell types [8,10]. Vaporized droplets were previously successfully imaged at clinically relevant acoustic pressure levels [11], which shows the potential to use these droplets not only as drug carriers but also for theranostics, which include imaging of drug delivery to the region of interest [12,13]. However, it is necessary to determine the mechanical properties of these droplets to develop an efficient ultrasound imaging sequence for visualization of droplets before and after drug delivery and to ensure that the drug is properly delivered to the region of interest.…”

Section: Introductionmentioning

confidence: 99%

Measuring the Compressibility of Cellulose Nanofiber-Stabilized Microdroplets Using Acoustophoresis

et al. 2021

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Droplets with a liquid perfluoropentane core and a cellulose nanofiber shell have the potential to be used as drug carriers in ultrasound-mediated drug delivery. However, it is necessary to understand their mechanical properties to develop ultrasound imaging sequences that enable in vivo imaging of the vaporization process to ensure optimized drug delivery. In this work, the compressibility of droplets stabilized with cellulose nanofibers was estimated using acoustophoresis at three different acoustic pressures. Polyamide particles of known size and material properties were used for calibration. The droplet compressibility was then used to estimate the cellulose nanofiber bulk modulus and compare it to experimentally determined values. The results showed that the acoustic contrast factor for these droplets was negative, as the droplets relocated to pressure antinodes during ultrasonic actuation. The droplet compressibility was 6.6–6.8 ×10−10 Pa−1, which is higher than for water (4.4×10−10 Pa−1) but lower than for pure perfluoropentane (2.7×10−9 Pa−1). The compressibility was constant across different droplet diameters, which was consistent with the idea that the shell thickness depends on the droplet size, rather than being constant.

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Section: Introductionmentioning

confidence: 99%

Measuring the Compressibility of Cellulose Nanofiber-Stabilized Microdroplets Using Acoustophoresis

et al. 2021

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“…Recently, perfluoropentane (PFP) droplets stabilized by cellulose nanofibers (CNFs) were developed by Ghorbani et al 11 The acoustic and mechanical properties of these droplets have been studied both in numerical and experimental studies; [25][26][27] however, the biological compatibility of these droplets is still unknown. The aim of this work was to study the effect of CNF-shelled PFP droplets on cancer cell viability, using murine breast cancer cell line 4T1 as a model.…”

Section: Introductionmentioning

confidence: 99%

Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study

Loskutova¹,

Torras²,

Ying³

et al. 2023

IJN

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To study the effect of cellulose nanofiber (CNF)-shelled perfluoropentane (PFP) droplets on the cell viability of 4T1 breast cancer cells with or without the addition of non-encapsulated paclitaxel. Methods: The CNF-shelled PFP droplets were produced by mixing a CNF suspension and PFP using a homogenizer. The volume size distribution and concentration of CNF-shelled PFP droplets were estimated from images taken with an optical microscope and analyzed using Fiji software and an in-house Matlab script. The thermal stability was qualitatively assessed by comparing the size distribution and concentration of CNF-shelled PFP droplets at room temperature (~22°) and 37°C. The cell viability of 4T1 cells was measured using a 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Additionally, a hemolysis assay was performed to assess blood compatibility of CNF-shelled PFP droplets. Results: The droplet diameter and concentration of CNF-shelled PFP droplets decreased after 48 hours at both room temperature and 37°C. In addition, the decrease in concentration was more significant at 37°C, from 3.50 ± 0.64×10 6 droplets/mL to 1.94 ± 0.10×10 6 droplets/mL, than at room temperature, from 3.65 ± 0.29×10 6 droplets/mL to 2.56 ± 0.22×10 6 droplets/mL. The 4T1 cell viability decreased with increased exposure time and concentration of paclitaxel, but it was not affected by the presence of CNF-shelled PFP droplets. No hemolysis was observed at any concentration of CNF-shelled PFP droplets. Conclusion: CNF-shelled PFP droplets have the potential to be applied as drug carriers in ultrasound-mediated therapy.

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Modeling Gasotransmitter Availability to Brain Capillary Endothelial Cells with Ultrasound-sensitive Microbubbles

Jourdain,

Chivukula,

Bashur

2023

Pharm Res

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A Study on the Acoustic Response of Pickering Perfluoropentane Droplets in Different Media

Cited by 4 publications

References 34 publications

Measuring the Compressibility of Cellulose Nanofiber-Stabilized Microdroplets Using Acoustophoresis

Measuring the Compressibility of Cellulose Nanofiber-Stabilized Microdroplets Using Acoustophoresis

Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study

Modeling Gasotransmitter Availability to Brain Capillary Endothelial Cells with Ultrasound-sensitive Microbubbles

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