A Study on the Different Methods of Preparation of Lutein from Supercritical Fluid Processed Lutein Esters

Abstract

Lutein is a dietary oxycarotenoid which is found to reduce the risks of Age-related Macular Degeneration (AMD). Supercritical fluid extraction of lutein esters from marigold petals was carried out and was found to be much effective than conventional solvent extraction. The saponification of pre-concentrated lutein esters to produce free lutein was studied which showed a composition of about 88% total carotenoids (UV-VIS spectrophotometry) and 90.7% lutein (HPLC). The lipase catalyzed hydrolysis of lutein esters in conventional medium was investigated. The optimal temperature, pH, enzyme concentration and water activity were found to be 50°C, 7, 15% and 0.33 respectively and the activity loss of lipase was about 25% after 8 times re-use at 50°C for 12 days. However, the lipase catalyzed hydrolysis of lutein esters in conventional media resulted in poor conversions (38.7%).

Quantitative Analysis Of Some Classes Of Bioactive Antioxidant Compounds In Fennel (Foeniculum Vulgare Mill.) Leaves

“…Among individual carotenoid compounds, β-carotene, lycopene, lutein (and lutein esters), zeaxanthin and cryptoxanthin were determined by extraction in acetone:hexane:petroleum ether, petroleum ether, ethanol, acetone and using the spectrophotometric methods of Braniša et al 2014, Sujith et al 2010, Butnariu et al 2014, Biehler et al 2010 The total concentration of phenolic compounds was determined by extracting plant tissue in methanol and reaction with Folin-Ciocâlteu reagent (10%) and sodium bicarbonate (7.5%) for 30 minutes. Absorbance was read at 765 nm against gallic acid calibration curve (Popoviciu et al 2020).…”