A Study on the Effect of Insulin on Dna, Rna and Protein Synthesis in Mouse Mammary Gland Tissue in Organ Culture

Wang, D. Y.; Amor, Vicky

doi:10.1677/joe.0.0500241

Cited by 11 publications

(3 citation statements)

References 21 publications

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“…The rates were determined over a 4-h period using [methyl-3H]thymidine, [5-3H]uridine, and [32P]orthophosphate at concentrations of 2, 1 and 10/tCi/ml respectively. Expiants were weighed and homogenized and either DNA and RNA were determined (Wang & Amor, 1971) or casein was isolated (Juergens, Stockdale, Topper & Elias, 1965); radioactivity was determined by liquid scintillation counting.…”

Section: Methodsmentioning

confidence: 99%

The Lactogenic Effects of Prolactin and Growth Hormone on Mammary Gland Explants From Virgin and Pregnant Sprague-Dawley Rats

Hallowes¹,

Wang²,

Lewis³

1973

Journal of Endocrinology

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Explants of mammary glands from mature virgin and pregnant Sprague\p=m-\ Dawley rats were maintained in organ culture for up to 96 h. The effects of insulin, corticosterone, ovine prolactin and bovine growth hormone on the synthesis of DNA, RNA and casein in the explants were studied. DNA synthesis in explants from virgin rats was maintained by insulin but was not increased by the addition of the other hormones tested. DNA synthesis in explants from pregnant rats was increased by insulin, and the addition of corticosterone and either prolactin or growth hormone to the culture medium increased this synthesis. The rate of RNA synthesis in explants from virgin rats was similar in medium 199 with or without additional hormones. RNA synthesis in explants from pregnant rats was increased by the addition of insulin or insulin plus corticosterone to the medium. In explants from both virgin and pregnant rats the maximal rate of hormone-stimulated DNA or RNA synthesis occurred during the first 24 h of culture.Casein synthesis, as measured by the uptake of32P-labelled orthophosphate by explants from virgin and pregnant rats, was increased by insulin plus corticosterone plus either prolactin or growth hormone. The rate of casein synthesis was maximal between 48 and 72 h and was reduced by actinomycin D. In the pregnant rats no significant differences were demonstrated between the effects of the hormones on DNA, RNA or casein synthesis in explants from the 5th, 10th, 16th or 19th day of pregnancy.

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Section: Methodsmentioning

confidence: 99%

The Lactogenic Effects of Prolactin and Growth Hormone on Mammary Gland Explants From Virgin and Pregnant Sprague-Dawley Rats

Hallowes¹,

Wang²,

Lewis³

1973

Journal of Endocrinology

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“…SIMPSON and SCHMIDT (1971) observed a 167 O/o increase in mammary D N A synthesis when progesterone, oestrogens and prolactin were injected together. Similarly, hydrocortisone, prolactin and insulin have been established to have a synergistic effect (RASKIN et al, 1973, VOYTOVICH et al, 1969and WANG and AMOR, 1971). These hormones have also been shown to have a synergistic effect on the mammary synthesis of protein (ANDERSON and RILLEMA, 1976).…”

Section: Discussionmentioning

confidence: 99%

Hormonal Effect on Protein Synthesis by the Rabbit Mammary Gland at Subcellular Level in vivo and in vitro

Singh

Ganguli

2010

Zentralblatt Für Veterinärmedizin Reihe A

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Summary The effect of hormones, of both ovarian and non‐ovarian origin, on protein synthesis by the mammary gland of virgin rabbits was studied. In vivo studies reveal that β‐oestradiol, progesterone, hydrocortisone, prolactin and insulin stimulate the PSA of the mammary gland at subcellular levels, hydrocortisone being the most effective. The microsomal fraction exhibited the highest stimulation in PSA. When all five hormones were administered together there was a synergistic effect. A similar synergistic effect by the hormones on PSA of the whole mammary preparation was also found in vitro. The stimulatory effect of hydrocortisone in vitro was greater than that of the other hormones; prolactin had very little effect. Zusammenfassung Hormonale Effekte auf die Proteinsynthese in der Milchdrüse (Milchdrüsenhomogenate) von Kaninchen bei in‐vitro‐ und in‐vivo‐Versuchen Bei virginellen Kaninchen wurde der Einfluß ovarieller und nichtovarieller Hormone auf die Proteinsynthese (PS) in der Milchdrüse untersucht. Bei in‐vivo‐Versuchen stimulierten β‐Oestradiol, Progesteron, Hydrocortison, Prolaktin und Insulin die PS im subzellulären Bereich (Drüsen‐homogenate), wobei Hydrocortison am stärksten wirkte. In der Mikrosomen‐fraktion wurde die PS am stärksten angeregt. Auch bei in‐vitro‐ Versuchen von Milchdrüsenpräparaten zeigten die er‐wähnten Hormone ähnliche synergistische Effekte auf die PS. In‐vitro‐Versuchen war der Stimulationseffekt des Hydrocortisons ebenfalls größer als derjenige der andern Hormone. Prolaktin zeigte dabei nur eine sehr geringe Wirkung. Résumé Effets hormonaux sur la synthèse des protéines dans la glande mammaire (homogénats de tissu mammaire) chez des lapins in vitro et in vivo On a recherché l'influence d'hormones ovariennes et non ovariennes sur la synthèse des protéines dans la glande mammaire de lapines vierges. β‐oestradiol, progestérone, hydrocortisone, prolactine et insuline ont stimulé in vivo la synthèse des protéines dans la région souscellulaire (homogénats de glande), l'hydrocortisone ayant agi le plus fortement. La synthèse des protéines fut induite le plus dans la fraction des microsomes. Les hormones mentionnées ont également montré des effets synergiques semblables sur la synthèse des protéines dans des recherches in vitro avec des préparations de glande mammaire. L'effet de stimulation de l'hydrocortisone fut également plus fort in vitro. La prolactine a montré seulement une faible action. Resumen Efectos hormonales sobre la síntesis proteica en la glándula mamaria (homogenados de las mismas glándulas) de conejas en ensayos in vitro e in vivo En conejas virginales se estudió el influjo de hormonas ováricas y no ováricas sobre la actividad de síntesis proteica (ASP) en la glándula mamaria. En ensayos in vivo estimulaban β‐oestradiol, progesterona, hidrocorti‐sona, prolactina e insulina la ASP en el ámbito subcelular (homogenados glandulares), presentando la hidrocortisona la actividad más pronunciada. La fracción microsomal exhibía la estimulación más intensa en la ASP. Cuando se ad...

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“…In particular, the maximal effects were observed after 24 h of culture, the time for the maximal stimu¬ lation of normal mammary gland (Wang & Amor, 1971;Hallowes, Wang & Lewis, 1973), though others have found maximal effects after 48 h (Heuson et al 1967;Lewis & Hal¬ lowes, 1974;Pasteéis et al 1976). We have been unable to demonstrate a consistent effect of hormone combinations containing progesterone, though others have reported the effectiveness of such combinations as insulin, prolactin, progesterone and oestradiol, or insulin, prolactin and progesterone in organ cultures of rat (Koyama, Sinha & Dao, 1972) and mouse (Mehta & Bannerjee, 1975) mammary gland and of rat mammary tumours (Pasteeis et al 1976).…”

Section: Sensitivity In Vitromentioning

confidence: 98%

Synthesis of Dna and Lecithin in Tissue Culture and Oestrogen Receptor Activity in Rat Mammary Tumours Dependent on and Independent of the Ovary

Scott¹,

Murphy²,

Hawkins³

1979

Journal of Endocrinology

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Dimethylbenz(alpha)anthracene (DMBA)-induced and transplanted rat mammary tumours (2 lines) were examined for oestrogen receptor activity, and for sensitivity to hormones in vivo (by ovariectomy) and in vitro (by tissue culture). In vivo, the growth of all tumours induced by the administration of DMBA in random-bred Sprague-Dawley rats was found to be dependent on the ovary, whilst in all transplanted tumours (12 TG-3 and six TG-5 lines), maintained in an inbred strain of Sprague-dawley rats, growth was found to be independent of the ovary. In vitro, the capacity for DNA synthesis in DMBA-induced tumours was better maintained after 24 h when insulin (10 microgram/ml) and corticosterone (5 microgram/ml) or insulin, corticosterone and prolactin (each 5 microgram/ml) were present in the medium (five out of 12 and eight out of 11 tumours respectively); no effect of hormones in the media was detected after 48 h. In the transplanted tumours, no effect of hormones on DNA synthesis was detected after either 24 or 48 h of culture. Synthesis of lecithin was not detectably influenced by the presence of hormones in either DMBA-induced or transplanted tumours. Oestrogen receptor concentrations were, on average, significantly higher in the DMBA-induced tumours than in either line of transplanted tumour. For 22 DMBA-induced tumours and 15 transplanted tumours, the effect of hormones in vitro ('response') was directly correlated with receptor concentration at time 0 (Spearman's rho = +0.59) and inversely correlated with the rate of DNA synthesis ('basal') at time 0 (Spearman's rho = -0.62). No single parameter or pair of parameters permitted accurate distinction between the tumour types.

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A Study on the Effect of Insulin on Dna, Rna and Protein Synthesis in Mouse Mammary Gland Tissue in Organ Culture

Cited by 11 publications

References 21 publications

The Lactogenic Effects of Prolactin and Growth Hormone on Mammary Gland Explants From Virgin and Pregnant Sprague-Dawley Rats

The Lactogenic Effects of Prolactin and Growth Hormone on Mammary Gland Explants From Virgin and Pregnant Sprague-Dawley Rats

Hormonal Effect on Protein Synthesis by the Rabbit Mammary Gland at Subcellular Level in vivo and in vitro

Synthesis of Dna and Lecithin in Tissue Culture and Oestrogen Receptor Activity in Rat Mammary Tumours Dependent on and Independent of the Ovary

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