Our hands do a wide variety of functions like touching, grasping, feeling, holding, manipulating, caring, and performing daily activities and more. They are a vitally important part of who we are and how we see ourselves. Hands are regarded as a major source of transmitting infection. Germs are found everywhere and easily spread through human hands, one of the most common vehicles of transmission of infection from one person to another and from one object to another to cause infection which increases the mortality and morbidity rate. Hand hygiene is important at every age especially in children. Hand washing is something everyone learns at a very early age and yet many people just don’t do it. The problem is that most and in particular young children don’t see it as a priority. Washing hands is the easiest way to stop germs from spreading and control transmission of infection. So every child should be aware of correct hand washing technique to prevent spread of diseases. Aims and Objectives: This study aims to assess the knowledge of the students on hand hygiene and provide knowledge to the students on hand hygiene through structured Teaching Programme. Research methodology: A quantitative research approach with pre experimental one group pre and post test design was adopted for the study. Out of total 300 students 30 students aged between 10-15 years of VI and VII standard (18 boys and 12 girls) in Janata Vidyalaya, Muliabari, Digboi chosen by using purposive convenient sampling technique. Pre test and post test on hand hygiene were conducted using knowledge questionnaire on hand hygiene before and after introducing the structured teaching programme on hand hygiene. Post test was conducted on 7th day of the pre-test conducted. Data analysis and interpretation of data was done by using Descriptive and Inferential statistics. Results: Out of 30 students 6(20%) study participant had adequate knowledge whereas 21(70%) had moderately adequate knowledge only 3(10%) had inadequate knowledge in pre-test. But in post-test out of 30 students 28(93.33%) study participants had adequate knowledge and 2(6.67%) had moderately adequate knowledge. Conclusion: The structured teaching programme was effective. Researchers may adopt structured teaching programme to do research studies on providing knowledge to the school students.