A Study to Evaluate Aflatoxin Contamination in Food from Gauteng Province

Abstract

Study Method Materials and ReagentsAflatoxins levels in food samples from Gauteng province were estimated by High Performance Liquid Chromatography (HPLC) and thin layer chromatography following an immune-affinity clean up. Aflatoxins extraction, detection and quantification were reached using the following materials and reagents that were of MERCK specifications except otherwise. They include: Separating funnels fitted with stoppers, large test tubes (15x25cm)/boiling tubes, wash bottles, elastic bands, methanol (HPLC grade), sodium chloride, nitric acid, potassium bromide, anhydrous sodium bicarbonate solution (saturated), acetonitrile, toluene, formic acid, Propan-2-ol, Ethyl acetate, dichloromethane, variable hot air drier (FENICI, 41512), TLC tank (Camag Ltd), Amber vials, HPLC vials, UV box, rotary blade blender (Torrington, CT. USA), phosphate buffered saline tablets (Prod codes: RP202), microfiber filter paper (Whatmann N o 113 (Prod codes: P66 and P67), aflaprep immunoaffinity columns (Prod code: AP01; R-Biopharm AG; Darmstadt, Germany), mobile phases, standards of aflatoxins (AFs) i.e. aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2 ), aflatoxin B 1 (AFB 1 ) and aflatoxin B 2 (AFB 2 ) (ARC, South Africa), 20 x 20 cm pre-coated aluminium backed silica gel G TLC plates Merck Art 5553, Aldrich), HPLC Spectra Physics SCM400 SYSTEM (Shimadzu Corporation, Kyoto,

A Focus on Aflatoxin in Feedstuffs: New Developments in Analysis and Detection, Feed Composition Affecting Toxin Contamination, and Interdisciplinary Approaches to Mitigate It

“…Other laboratories have recently applied these concrete columns to monitor aflatoxin in food and feed [50,51]. Multiple columns (e.g.…”