Regular whole blood donations sometimes may result in iron (Fe) inadequacy in the blood donors, which can lead to increased deferral rate among the donors. Blood collection centers must keep a track record of Fe status of regular whole blood donors, as well the impact it has on donor health. The blood centers must also counsel the donors regarding measures to improve the low Fe stores. Serum ferritin levels have been a better indicator of low Fe stores in the body as compared to hemoglobin. There is presently no international agreement on an acceptable approach for Fe monitoring. There are several ways to check the Fe levels in blood of whole blood donors. Hemoglobin- or ferritin-levels and Fe supplementation could be accessed to check the impact of regular blood donations. However, employing an Fe assessment method may provide new confrontations in management and accessibility of blood donors. We present an overview of parameters monitored for Fe stores in regular whole blood donors.