A sub‐acute study of metronidazole toxicity assessed in Egyptian <i>Tilapia zillii</i>

Khalil, Wagdy K. B.; Mahmoud, Mahmoud; Zahran, M. M.; Mahrous, Karima F.

doi:10.1002/jat.1217

Cited by 13 publications

(8 citation statements)

References 46 publications

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“…MTZ is generally considered to be non-toxic, with no evidence for developmental toxicity [41], although genotoxicity and neurotoxicity have been reported [42], [43]. The ability to expose embryos to lower concentrations of MTZ, and at earlier stages of development, expands the possibilities of this model for use in other developmental biology and toxicology studies, in particular at early developmental time points, as well as during normal hepatic development.…”

Section: Discussionmentioning

confidence: 99%

Investigating the application of a nitroreductase-expressing transgenic zebrafish line for high-throughput toxicity testing

Chlebowski

Truong

et al. 2017

Toxicology Reports

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Nitroreductase enzymes are responsible for the reduction of nitro functional groups to amino functional groups, and are found in a range of animal models, zebrafish (Danio rerio) excluded. Transgenic zebrafish models have been developed for tissue-specific cell ablation, which use nitroreductase to ablate specific tissues or cell types following exposure to the non-toxic pro-drug metronidazole (MTZ). When metabolized by nitroreductase, MTZ produces a potent cytotoxin, which specifically ablates the tissue in which metabolism occurs. Uses, beyond tissue-specific cell ablation, are possible for the hepatocyte-specific Tg(l-fabp:CFP-NTR)s891 zebrafish line, including investigations of the role of nitroreductase in the toxicity of nitrated compounds. The hepatic ablation characteristics of this transgenic line were explored, in order to expand its potential uses. Embryos were exposed at 48, 72, or 96 hours post fertilization (hpf) to a range of MTZ concentrations, and the ablation profiles were compared. Ablation occurred at a 10-fold lower concentration than previously reported. Embryos were exposed to a selection of other compounds, with and without MTZ, in order to investigate alternative uses for this transgenic line. Test compounds were selected based on: their ability to undergo nitroreduction, known importance of hepatic metabolism to toxicity, and known pharmaceutical hepatotoxins. Selected compounds included nitrated polycyclic aromatic hydrocarbons (nitro-PAHs), the PAHs retene and benzo[a]pyrene, and the pharmaceuticals acetaminophen and flutamide. The results suggest a range of potential roles of the liver in the toxicity of these compounds, and highlight the additional uses of this transgenic model in toxicity testing.

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Section: Discussionmentioning

confidence: 99%

Investigating the application of a nitroreductase-expressing transgenic zebrafish line for high-throughput toxicity testing

Chlebowski

Truong

et al. 2017

Toxicology Reports

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“…Tissue samples were processed routinely for paraffin sections of 4–5 μm thickness. Haematoxylin and eosin were used for the straining of these liver tissues (Khalil et al., 2007).…”

Section: Methodsmentioning

confidence: 99%

Toxicological and therapeutic effects of neem ( Azadirachta indica ) leaf powder in hole‐in‐the‐head (HITH) disease of fish Anabas testudineus

Mondal¹,

Garai

Chatterjee

et al. 2020

Aquac. Res.

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“…Complete Poly(A)+ RNA isolated from brain tissues was reverse transcribed (RT) into cDNA in a total volume of 20 μl using RevertAid™ First Strand cDNA Synthesis Kit (Fermentas, Germany). The reaction tubes containing RT preparations were flash-cooled in an ice chamber until being used for cDNA amplification through quantitative Real-time polymerase chain reaction (qPCR) [17], [18].…”

Section: Methodsmentioning

confidence: 99%

Effect of natural PAL-enzyme on the quality of egg white and mushroom flour and study its impact on the expression of PKU related genes and phenylalanine reduction in mice fed on

Eissa

Abdallah

Khalil

et al. 2017

Journal of Genetic Engineering and Biotechnology

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PKU patients react to therapy with a low phenylalanine diet, but adherence to this diet is troublesome, subsequently the expansion of alternative ways is demand. Phenylalanine ammonia lyase (PAL) is one of this ways, which converts phenylalanine to harmless metabolites; trans-cinnamic acid and ammonia. In the current study, the extraction of PAL enzyme was used to investigate the efficiency for production of functional PKU egg white and mushroom flour with good quality by evaluation of colour characteristics, determination of phenylalanine concentrations and genetic materials expression of PKU related genes and DNA damage. Results indicated that the PAL enzyme treated of egg white and mushroom flour was stable colour and the calculated reduction per cent in phenylalanine concentration from female mice fed on untreated and PAL–treated samples was 22.77% in egg white and 31.37% in mushroom flour. Also, the results revealed that female mice fed on diet contained treated egg white exhibited low expression levels of PKU exons (3, 6, 7, 11, and 12) and low DNA damage which were similar to those in control mice.

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A sub‐acute study of metronidazole toxicity assessed in Egyptian Tilapia zillii

Cited by 13 publications

References 46 publications

Investigating the application of a nitroreductase-expressing transgenic zebrafish line for high-throughput toxicity testing

Investigating the application of a nitroreductase-expressing transgenic zebrafish line for high-throughput toxicity testing

Toxicological and therapeutic effects of neem ( Azadirachta indica ) leaf powder in hole‐in‐the‐head (HITH) disease of fish Anabas testudineus

Effect of natural PAL-enzyme on the quality of egg white and mushroom flour and study its impact on the expression of PKU related genes and phenylalanine reduction in mice fed on

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