Ligament injury is one of the common diseases with the highest incidence in sports diseases, which seriously affects people's physical exercise. Once the ligament is injured, the body's automatic repair ability cannot achieve self-repair. In recent years, with the rapid development of tissue engineering, its application range gradually involves the medical field. The ligament prepared by tissue engineering has the advantages of antiinfection and will not cause immune rejection with the human body. The replacement of damaged artificial ligaments by tissue engineered ligaments can enhance the self-healing ability of ligaments to a certain extent. In addition, the postoperative recovery process also has an impact on the overall treatment effect. As an important nursing method in ligament treatment, tissue engineered ligament repair can circulate blood, strengthen muscles and improve joint flexibility. Based on this, this article proposes a preparation method based on tissue-engineered ligaments to repair sports exercise injuries. The research in this article is mainly divided into three parts: The first part is the study of tissue engineering on ligament damage, whether the newly prepared ligament will rub against the human body after being implanted in the human body, and the ability of tissue engineering to repair ligament damage is evaluated;The second part of it is the study of tissue engineering in the treatment of ligament injuries, which explored the mechanism of Tai Chi to enhance the recovery ability of patients. The third part is a clinical study, which draws conclusions based on the comparison of various indicators between the observation group and the control group. The results of this experiment show that in the clinical repair treatment of ligament injuries, the ligaments prepared by tissue engineering have good antibacterial properties and good histocompatibility with human bones because of the endophytes. The observation group combined with Tai Chi exercise therapy had minor complications, such as swelling, repeated dislocations and contractures, which could effectively avoid repeated injuries to the patients' ligaments.