A submerged culture system for rapid micropropagation of the commercially important aquarium plant, ‘Amazon sword’ (Echinodorus ‘Indian Red’)

Haque, Sk Moquammel; Ghosh, Biswajit

doi:10.1007/s11627-018-9938-6

Cited by 10 publications

(2 citation statements)

References 19 publications

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“…The present finding further indicated that the inclusion of an auxin (IAA or NAA) along with the optimal cytokinin (BAP) concentration improved the multiplication rate. Other research groups also found that the combination of BAP and NAA proved best for the micropropagation of some other ornamental aquatic plants, such as Anubias Heterophylla [16], Aponogeton ulvaceus Baker [32], Echinodorus 'Indian Red' [33], and Lindernia antipoda (L.) Alston [34], as well as for the medicinal herb Artemisia abrotanum L. [35]. However, these findings disagree with those of Sheeja et al [36], who reported that BAP alone proved better than the combination of BAP and NAA for the micropropagation of an ornamental aquatic plant Anubias barteri Var.…”

Section: (C) and 2(d))mentioning

confidence: 97%

Rapid Clonal Multiplication Through In Vitro Axillary Shoot Proliferation of Lobelia cardinalis L., an Ornamental Aquatic Plant

Klaocheed,

Rittirat,

Kalawong

et al. 2024

Trends Sci

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Aquatic plants are grown in aquaria for their beauty and to maintain the quality of water. The explants of Lobelia cardinalis L. were obtained from the Aquatic Plant Center Co., Ltd., Thailand. In vitro clonal propagation offers alternative strategy for its rapid clonal multiplication. In this study, multiple and rapid propagations of Lobelia cardinalis L. with tissue culture techniques were aimed. Highly efficient and repeatable in vitro regeneration protocol was established from nodal segments of Lobelia cardinalis L. Various concentrations and combinations of various plant growth regulators (PGRs) were employed to induce multiple shoots, shoot elongation, number of leaves and rooting of shoots to obtain complete plantlets of Lobelia cardinalis L. Among 2 PGRs such as 6-benzylaminopurine (BAP) and α-Naphthalene acetic acid (NAA) tested for multiple shoot induction. Regenerated shoots had a higher number of shoots on MS medium contained cytokinins with auxins. The maximum shoot proliferation (100 %), number of shoots per explant (6.20 ± 0.51 shoots), shoot length (11.67 ± 0.69 mm) and number of leaves per explant (58.10 ± 0.38 leaves per explant) were observed in MS medium supplemented with 1.0 mg/L BAP and 2.0 mg/L NAA. Rooting was achieved within 3 to 4 weeks on all the rooting media, but MS medium augmented with 1.0 mg/L indole-3-butyric acid (IBA) produced the maximum number of strong and healthy roots (11.70 ± 0.27 roots). The regenerated complete plantlets with healthy roots and shoot system were transferred to 3.5 cm diameter black net plastic pots containing rockwool medium and successfully acclimatized with a survival rate of 100 % in the greenhouse and no phenotypic variations were observed among regenerated plants. The success of this protocol offers highly efficient method of the plant multiplication, which would be beneficial for plant tissue culturist, and nursery people where regular supply of plants is a large number required. This protocol can be utilized for commercial scale propagation. HIGHLIGHTS Micropropagation was established from nodal segments of Lobelia cardinalis L The maximum shoot proliferation, number of shoots per explant, shoot length and number of leaves per explant were observed in MS medium supplemented with 1.0 mg/L BAP and 2.0 mg/L NAA MS medium augmented with 1.0 mg/L indole-3-butyric acid (IBA) produced the maximum number of roots The regenerated plantlets were successfully acclimatized with a survival rate of 100 % in the greenhouse GRAPHICAL ABSTRACT

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Section: (C) and 2(d))mentioning

confidence: 97%

Rapid Clonal Multiplication Through In Vitro Axillary Shoot Proliferation of Lobelia cardinalis L., an Ornamental Aquatic Plant

Klaocheed,

Rittirat,

Kalawong

et al. 2024

Trends Sci

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“…Therefore, the adjustment of basal medium strength is a simple but effective way to adjust the molar concentrations of several nutrients simultaneously. For example, the use of different strengths of a popular basal medium, Murashige and Skoog (MS), showed an AR formation gradient from Echinodorus 'Indian Red' shoots: 1/2 > 3/4 > 1/4 > full-strength [20], or 1/2 > 3/4 > full > 1/4-strength for Ledebouria revoluta bulblets [21]. Half-strength Woody Plant Medium (WPM) [22] free of PGRs induced signi cantly more ARs from Salix tetrasperma shoots than full-strength WPM [23].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng

Xiong,

Chen,

Liu

et al. 2024

Preprint

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Background The extract from Metasequoia glyptostroboides Hu et Cheng, a rare and endangered species native to China, exhibits numerous biological and pharmacological activities. The species is recalcitrant to rooting during micropropagation, a challenge that has yet to be resolved. In this study, transcriptomic and hormonal analyses were conducted to appreciate the molecular mechanism of adventitious root (AR) formation in optimized rooting conditions. Results The use of 2/5-strength Woody Plant Medium (WPM) significantly promoted AR formation of M. glyptostroboides shoots while the content of endogenous auxin, cytokinins and gibberellins (GAs) varied at different stages of AR formation. Transcriptomic analysis showed the significant up- or down-regulation of differentially expressed genes (DEGs) associated with plant hormone signal transduction and the phenylpropanoid biosynthesis pathway in response to 2/5-strength WPM. DEGs related to the biosynthesis of indole-3-acetic acid, cytokinins and GAs were identified. Transcript factors involved in 13 families were also revealed. A weighted gene co-expression network analysis indicated a strong correlation between hormones and genes involved in plant hormone signal transduction and the phenylpropanoid biosynthetic pathway. Conclusions These results indicate that the AR-promoting potential of 2/5-strength WPM in M. glyptostroboides was due to complex interactions between hormones and the expression of genes related to plant hormone signal transduction and the phenylpropanoid biosynthetic pathway.

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An efficient method for economic micropropagation of three aquatic plant species (Lobelia cardinalis, Staurogyne repens, and Alternanthera reineckii)

2022

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A submerged culture system for rapid micropropagation of the commercially important aquarium plant, ‘Amazon sword’ (Echinodorus ‘Indian Red’)

Cited by 10 publications

References 19 publications

Rapid Clonal Multiplication Through In Vitro Axillary Shoot Proliferation of Lobelia cardinalis L., an Ornamental Aquatic Plant

Rapid Clonal Multiplication Through In Vitro Axillary Shoot Proliferation of Lobelia cardinalis L., an Ornamental Aquatic Plant

Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng

An efficient method for economic micropropagation of three aquatic plant species (Lobelia cardinalis, Staurogyne repens, and Alternanthera reineckii)

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