A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors.

Rolink, A G; Boekel, Edwin ten; Melchers, Fritz; Fearon, D T; Krop, Ian E.; Andersson, Jan

doi:10.1084/jem.183.1.187

Cited by 210 publications

(164 citation statements)

References 44 publications

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“…6, A and B). CD4 Ϫ DX5 Ϫ CD19 Ϫ B220 ϩ bone marrow cells, which include early B progenitors (37,38), expressed low levels of CCR9 and could respond to CCL25 (Fig. 6B and data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, CD4 ϩ DX5 Ϫ CD19 Ϫ B220 ϩ cells were found to express high levels of CCR9 and could respond to CCL25. The lineage affiliation and differentiation potential of CD4 ϩ DX5 Ϫ CD19 Ϫ B220 ϩ cells remain unclear, although phenotypically similar cells from bone marrow that respond to CCL25 contain both B and T cell progenitors (36,37,39,40). To investigate whether CCR9 is involved in regulating the migration of T-progenitor cells to thymus, we performed competitive bone marrow transplantation experiments.…”

Section: Discussionmentioning

confidence: 99%

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A Role for CCR9 in T Lymphocyte Development and Migration

Uehara

Grinberg

Farber

et al. 2002

The Journal of Immunology

295

302

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CCR9 mediates chemotaxis in response to CCL25/thymus-expressed chemokine and is selectively expressed on T cells in the thymus and small intestine. To investigate the role of CCR9 in T cell development, the CCR9 gene was disrupted by homologous recombination. B cell development, thymic αβ-T cell development, and thymocyte selection appeared unimpaired in adult CCR9-deficient (CCR9−/−) mice. However, competitive transplantation experiments revealed that bone marrow from CCR9−/− mice was less efficient at repopulating the thymus of lethally irradiated Rag-1−/− mice than bone marrow from littermate CCR9+/+ mice. CCR9−/− mice had increased numbers of peripheral γδ-T cells but reduced numbers of γδTCR+ and CD8αβ+αβTCR+ intraepithelial lymphocytes in the small intestine. Thus, CCR9 plays an important, although not indispensable, role in regulating the development and/or migration of both αβ− and γδ− T lymphocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Role for CCR9 in T Lymphocyte Development and Migration

Uehara

Grinberg

Farber

et al. 2002

The Journal of Immunology

295

302

View full text Add to dashboard Cite

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“…Two additional pDC markers (Ly6C [24] and mPDCA-1), expressed on both Ly49Q + and Ly49Q -pDC from the bone marrow (Fig. 4B), were used to verify the identity of the pDC; contamination by NK cell precursors, which also express CD11c and B220 [25,26], was ruled out by staining for CD122, another marker of NK cell precursors [27] (Fig. 4B).…”

Section: Bone Marrow Cells Cultured With Flt3l Secrete Type I Ifnmentioning

confidence: 99%

Type I interferon regulates pDC maturation and Ly49Q expression

Toma-Hirano

Namiki²,

Miyatake

et al. 2007

Eur J Immunol

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Ly49Q is expressed on peripheral mouse plasmacytoid dendritic cells (pDC). Immature Ly49Q-negative pDC precursors acquire Ly49Q in the bone marrow and then migrate into the periphery. While searching for molecules that regulate pDC maturation, we found that type I interferon (IFN) inhibited Ly49Q acquisition in vitro. Infections that induce type I IFN production by cells other than pDC (a condition mimicked by poly(I:C) injection in vivo) increase the prevalence of Ly49Q -pDC in the bone marrow and peripheral lymphoid organs in wild-type but not IFN-a/b receptor knockout BALB/c mice. Moreover, in vivo exposure to type I IFN causes some Ly49Q -, but not Ly49Q + , pDC to convert to conventional DC, defined as B220 -CD11c + CD11b + cells. These data suggest that type I IFN regulates pDC development and affects their distribution in the body.

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“…The functional behaviour of NK cells is regulated by the engagement of a broad array of activating and inhibitory cell membrane receptors (reviewed in Lanier [12]). The BM is considered to be the main site for NK cell development [13][14][15][16]. Here, multipotent haematopoietic precursors generate NK cell precursors (NKP).…”

mentioning

confidence: 99%

“…The BM is considered to be the main site for NK cell development [13][14][15][16]. Here, multipotent haematopoietic precursors generate NK cell precursors (NKP).…”

mentioning

confidence: 99%

Continuous CD27 triggering in vivo strongly reduces NK cell numbers

et al. 2010

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NK cells are important mediators of the early defense. In mice, immature and mature NK (mNK) cells constitutively express the TNF receptor family member CD27; however, mNK cells eventually lose CD27 expression and become resting NK cells. Interaction of CD27 with its ligand, CD70, enhances proliferation and effector functions of NK cells. We used mice that constitutively express CD70 on B cells (CD70-Tg) to study the in vivo effects of continuous triggering of CD27 on NK cells. Continuous CD70-CD27 interaction resulted in strongly down-modulated CD27 expression on NK cells and gradually reduced absolute NK cell numbers. This reduction was most prominent in the mNK cell subpopulation and was at least partially due to increased apoptosis. Residual NK cells showed lower expression of activating Ly49 receptors and normal (liver) or decreased (spleen) IFN-c production. Nevertheless, NK cells from CD70-Tg mice displayed higher YAC-1 killing capacities. CD70-Tg NK cells exhibited up-regulated expression of NKG2D, which is in accordance with the increased YAC-1 lysis, as this is mainly NKG2D-dependent. Taken together, this study is the first to demonstrate that continuous CD70 triggering of CD27 on NK cells in vivo results in a severe reduction of NK cells. On a single cell basis, however, residual NK cells display enhanced cytotoxicity.Key words: CD70-Tg mice . Cytotoxicity . Differentiation Supporting Information available online Introduction NK cells are large granular lymphocytes of the innate immune system that play a crucial role in the early host defense [1,2]. Upon activation, they directly eliminate target cells through exocytosis of perforin-and granzyme-containing granules, or by Fas ligand (CD178) or TRAIL pathways [3][4][5][6][7]. NK cells also produce cytokines and chemokines, which enable them to recruit non-specific haematopoetic cells, activate dendritic cells and prime adaptive lymphocytes [8][9][10][11]. As such, NK cells bridge between innate and adaptive immunity. The functional behaviour of NK cells is regulated by the engagement of a broad array of activating and inhibitory cell membrane receptors (reviewed in Lanier [12]).The BM is considered to be the main site for NK cell development [13][14][15][16]. Here, multipotent haematopoietic precursors generate NK cell precursors (NKP). Murine NKP are lineage(lin) À CD122 1 NK1.1 À CD49b À . NKP differentiate into immature NK (iNK) cells, which exhibit a lin À CD122 1 NK1. [20,21]. Interestingly, Hayakawa and Smyth [22]showed that within the TCR b À NK1.1 1 gated NK cell pool there is a CD11b low subpopulation, including both iNK and early mNK cells, which is homogenously CD27 high (referred to as subset 1), whereas the CD11b high population of late mNK cells consists of two functionally distinct subsets: i.e. CD27 high (referred to as subset 2) and CD27 low (referred to as subset 3). NK cells from subset 1 are the first NK cell population detected after BM transplantation and they give rise to subset 2 after adoptive transfer. Subset 2 consists of fu...

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A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors.

Cited by 210 publications

References 44 publications

A Role for CCR9 in T Lymphocyte Development and Migration

A Role for CCR9 in T Lymphocyte Development and Migration

Type I interferon regulates pDC maturation and Ly49Q expression

Continuous CD27 triggering in vivo strongly reduces NK cell numbers

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