A success in Toxinology translational research in Brazil: Bridging the gap

Ferreira, Ana Sílvia Sartori Barraviera Seabra; Barraviera, Benedito; Barraviera, Sílvia Regina Catharino Sartori; Abbade, Luciana Patrícia Fernandes; Caramori, Carlos Antonio; Ferreira, Rui Seabra

doi:10.1016/j.toxicon.2013.01.003

Cited by 11 publications

(10 citation statements)

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“…Several in vivo studies have been performed in different animal species and humans, demonstrating the translational potential of our FS [16,30,31]. …”

Section: Discussionmentioning

confidence: 99%

A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

et al. 2014

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IntroductionThe optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the ability of cells to affect and detect the biological microenvironment.MethodsThe characterization of CD34, CD44 and CD90 expression on mesenchymal stem cells was performed by flow cytometry. In vitro growth and cell viability were evaluated by light and electron microscopy. Differentiation into osteogenic, adipogenic and chondrogenic lineages was induced.ResultsThe fibrin sealant did not affect cell adhesion, proliferation or differentiation and allowed the adherence and growth of mesenchymal stem cells on its surface. Hoechst 33342 and propidium iodide staining demonstrated the viability of mesenchymal stem cells in contact with the fibrin sealant and the ability of the biomaterial to maintain cell survival.ConclusionsThe new fibrin sealant is a three-dimensional scaffolding candidate that is capable of maintaining cell survival without interfering with differentiation, and might also be useful in drug delivery. Fibrin sealant has a low production cost, does not transmit infectious diseases from human blood and has properties of a suitable scaffold for stem cells because it permits the preparation of differentiated scaffolds that are suitable for every need.

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“…Several in vivo studies have been performed in different animal species and humans, demonstrating the translational potential of our FS [16,30,31]. …”

Section: Discussionmentioning

confidence: 99%

A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

et al. 2014

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“…Discovering new in vitro hit compounds might be considered relatively easier than crossing the animal-to-human barrier, which is still a great challenge to drug discovery programs. Bridging this gap between clinical and basic research must be encouraged by health professionals in order to discover effective treatments, especially for neglected diseases [ 5 ]. The search to develop alternative compounds, either of natural or synthetic origin, effective for both medical therapy and chemoprophylaxis, should be carefully considered [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response

Pereira¹,

Barros²,

Pinto³

et al. 2016

J Venom Anim Toxins Incl Trop Dis

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BackgroundApis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases.MethodsAfricanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined.ResultsPromastigotes and intracellular amastigotes were susceptible to melittin (IC50 28.3 μg.mL−1 and 1.4 μg.mL−1, respectively), but also showed mammalian cell cytotoxicity with an IC50 value of 5.7 μg.mL−1. Uninfected macrophages treated with melittin increased the production of IL-10, TNF-α, NO and H2O2. Infected melittin-treated macrophages increased IL-12 production, but decreased the levels of IL-10, TNF-α, NO and H2O2.ConclusionsThe results showed that melittin acts in vitro against promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Furthermore, they can act indirectly on intracellular amastigotes through a macrophage immunomodulatory effect.

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“…Translational medicine basically aims to facilitate the integration of basic research with clinical research, with the aim of transferring the applicability of its benefi ts to the population as a whole [1,2]. According to Lean et al, "It is a process that part of the evidence-based medicine towards sustainable solutions to community health problems" [3].…”

Section: Short Communicationmentioning

confidence: 99%