A successful pregnancy and live birth after intracytoplasmic sperm injection with globozoospermic sperm and electrical oocyte activation

Egashira, A.; Murakami, Masao; Haigo, Kosuke; Horiuchi, Toshitaka; Kuramoto, Takeshi

doi:10.1016/j.fertnstert.2009.08.013

Cited by 58 publications

(42 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although previous investigations could determine DNA fragmentation level in very few patients (Table 2), in terms of quantity, this is the largest study in fully globozoospermic men, performed to find out any correlation between DNA damage level and CMA3 positive sperm cells with round head. Several studies were so far able to evaluate DNA damage level in only one patient, using different techniques including SCSA and comet assay (Larson et al, 2001), TUNEL assay (Vicari et al, 2002;Taylor et al, 2010;Perrin et al, 2011b;Sermondade et al, 2011), SCD (Tejera et al, 2008), and AOT (Egashira et al, 2009). Compared with those of fertile controls in Larson's and Sermondade's studies, further investigations on two and six *Correction added on 26 May 2015, after first online publication: sentence structure corrected.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

et al. 2015

View full text Add to dashboard Cite

SUMMARYThe absence of the acrosome causes the situation which is called globozoospermia. There are a few studies, mostly as case reports, about correlation between levels of sperm DNA damage in patients with total round-headed spermatozoa. We investigated this correlation as well as CMA3 positive spermatozoa in 20 globozoospermic men (with more than 90% round-headed spermatozoa) attending to Royan Institute. Semen samples divided into three parts to semen analysis, to measure DNA fragmentation index (DFI) using sperm chromatin structure assay (SCSA) and to detect CMA3 + sperm cells by chromomycin A3 staining and fluorescent microscopy. Our results showed that there were significant differences in sperm concentration, total sperm motility, and normal morphology between patients and controls group (p < 0.001). Moreover, the average of DFI and CMA3 positive spermatozoa in patients group significantly increases compared with control group (p < 0.001). A significant correlation between DFI and CMA3 + in total population was also detected in patients group (r = 0.45, p = 0.046). To our knowledge, this is the largest study about correlation between DNA damage levels and CMA3 positive spermatozoa with round head sperm cells in total globozoospermic men. It seems that the increase in DNA damage may be because of defective sperm DNA compaction, as we detected CMA3 positive sperm cells in these patients.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Although, there have been some studies about correlation between round head spermatozoa and high levels of sperm DNA damage and protamine deficiency (Tejera et al, 2008;Egashira et al, 2009;Brahem et al, 2011;Sermondade et al, 2011), these reports were in limited number of patients, mostly in a case report.…”

Section: Introductionmentioning

confidence: 99%

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In total globozoospermia, a reduced ability to fertilize is described in about one-half of the cases reported, for which oocyte activation is applied in various reports (5)(6)(7)(8)(9)(10)(11)16). Because a previous study from our group (1) demonstrated an increased occurrence of structural abnormalities in ''normal'' oval spermatozoa derived from PG men, we anticipated that these ''normal cells'' that are picked for ICSI could have the impaired ability to fertilize in common with total globozoospermia.…”

Section: Discussionmentioning

confidence: 99%

“…The poor fertilization rates with ICSI may be due to deficient oocyte activation by these spermatozoa (4). Oocyte activation with calcium ionophores or electric activation has been applied in some studies and resulted in better fertilization rates in most cases (3,(5)(6)(7)(8)(9)(10)(11). To our knowledge, no augmented risk on congenital abnormalities has been reported in such pregnancies.…”

mentioning

confidence: 99%

Intracytoplasmic sperm injection in partial globozoospermia

Dam

Pijnenburg

Hendriks

et al. 2012

Fertility and Sterility

View full text Add to dashboard Cite

“…The causes of poor morphology can be related to lifestyle and habits, such as smoking and toxin exposure. Teratozoospermia, including the globozoospermia type (Egashira et al, 2009), may be treated by intracytoplasmic sperm injection that injects sperm directly into the egg (French et al, 2010) for optimal success rates.…”

Section: Introductionmentioning

confidence: 99%

Systematic tracking of altered modules identifies disrupted pathways in teratozoospermia

Huang

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The objective of this study was to identify disrupted pathways in teratozoospermia by systematically tracking dysregulated modules in reweighted protein-protein interaction (PPI) networks. We inferred and reweighted the PPI networks of normal and teratozoospermia groups based on Spearman correlation coefficients. Modules in the PPI networks were explored via a clique-merging algorithm and altered modules were identified based on maximum weight bipartite matching. Furthermore, pathway-enrichment analyses of genes in altered modules were performed by Database for Annotation, Visualization, and Integrated Discovery (DAVID) to illuminate the biological pathways in teratozoospermia. A total of 20,102 genes were screened from the expression profile. We explored 2406 and 2101 modules in normal and disease PPI networks, respectively. Moreover, we obtained 875 altered modules by comparing modules in normal and teratozoospermia PPI networks. At P < 0.01, the genes involved in 2855 interactions with score changes >1 were mainly enriched in 66 pathways and the genes in altered modules were enriched in 71 pathways. The activity genes (missed and added genes in the disrupted modules) were enriched in 41 common pathways. There were 36 mutual enriched pathways under the five different conditions. Moreover, the cell cycle pathway was disrupted in the first 10 pathways of each condition. This study provides a powerful biomarker discovery platform to better understand the progression of teratozoospermia by systematically tracking dysregulated modules. This method uncovered potential diagnostic and therapeutic targets of teratozoospermia. This information might lead to improved monitoring and treatment of teratozoospermia.

show abstract

A successful pregnancy and live birth after intracytoplasmic sperm injection with globozoospermic sperm and electrical oocyte activation

Cited by 58 publications

References 27 publications

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

Intracytoplasmic sperm injection in partial globozoospermia

Systematic tracking of altered modules identifies disrupted pathways in teratozoospermia

Contact Info

Product

Resources

About