A Sugar-Inducible Protein Kinase, VvSK1, Regulates Hexose Transport and Sugar Accumulation in Grapevine Cells

Lecourieux, Fatma; Lecourieux, David; Vignault, Ceݩline; Delrot, Serge

doi:10.1104/pp.109.149138

Cited by 63 publications

(55 citation statements)

References 79 publications

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“…The overexpression of the VvSK1 gene in transgenic 41b grapevine cells enhances the expression of four monosaccharide transporters (VvHT3-6) and leads to a three-to five-fold increase in glucose uptake by the cells, doubling the amounts of glucose and sucrose in the cells. Altogether, these data suggest that VvSK1 protein might be a key element for the regulation of hexose accumulation in flesh cells of berries (Lecourieux et al 2010). …”

Section: Sugar Concentrationmentioning

confidence: 78%

“…VvMSA, which is induced by ABA and sugars, is a transcriptional regulator controlling VvHT1 expression (Cakir et al 2003). Recently, a cDNA encoding a GK3/shaggy-like protein kinase, VvSK1 (V. vinifera shaggy-like protein kinase 1) has been isolated and functionally characterized in our laboratory (Lecourieux et al 2010). VvSK1 is strongly expressed postveraison, when the berries accumulate hexoses, and in grapevine cell suspensions, VvSK1 transcript abundance is increased by sucrose or ABA treatments.…”

Section: Sugar Concentrationmentioning

confidence: 99%

See 1 more Smart Citation

Ecophysiological, Genetic, and Molecular Causes of Variation in Grape Berry Weight and Composition: A Review

Dai

Ollat

Gomès

et al. 2011

Am. J. Enol. Vitic.

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197

137

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Berry fresh weight and composition are under the control of complex interactions among genotype, environmental factors, and viticulture practice, which all affect not only the mean value but also the ranges of variation in berry traits. Both mean values and variation range in berry composition play a role in berry quality and, subsequently, wine typicity. This review examines recent ecophysiological, genetic, and molecular knowledge to provide better understanding of the mechanisms that influence variability in berry weight and composition. We specifically reviewed the variation range in berry weight and composition (including sugars, organic acids, and anthocyanins) among Vitis genotypes, the environmental and viticulture practices that cause variability for a given cultivar, the genetic clues underlying the genotypic variation, and the putative genes controlling berry weight and composition. Despite numerous studies comparing differences in the mean value of a berry trait among different environment conditions and viticulture practices, very few studies have explored the level of variation in response to those factors. Present genetic and molecular studies are mainly focused on identifying genes involved in the control of berry weight and composition, with few considerations of environmental factors that affect their expression. In the future, more effort should be directed toward integration of genetic and molecular work with ecophysiological approaches in an effort to gain novel insights into the cause of variability in grape fresh weight and composition.

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Section: Sugar Concentrationmentioning

confidence: 78%

Section: Sugar Concentrationmentioning

confidence: 99%

Ecophysiological, Genetic, and Molecular Causes of Variation in Grape Berry Weight and Composition: A Review

Dai

Ollat

Gomès

et al. 2011

Am. J. Enol. Vitic.

Self Cite

197

137

View full text Add to dashboard Cite

show abstract

“…Grapevine transformations were made in 41B rootstock (cv Chasselas 3 Vitis berlandieri) according to Lecourieux et al (2010). An embryogenic cell suspension culture was initiated as described previously (Coutos-Thévenot et al, 1992a, 1992b.…”

Section: Plant Transformation and Culture Conditionsmentioning

confidence: 99%

“…Total RNA from grape organs and berries was extracted according to Lecourieux et al (2010). Total RNA from grape cells was extracted from 100 mg of starting tissue using the Spectrum Plant Total RNA Kit (Sigma) following the manufacturer's protocol.…”

Section: Plant Transformation and Culture Conditionsmentioning

confidence: 99%

The Basic Leucine Zipper Transcription Factor ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 Is an Important Transcriptional Regulator of Abscisic Acid-Dependent Grape Berry Ripening Processes

et al. 2013

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In grape (Vitis vinifera), abscisic acid (ABA) accumulates during fruit ripening and is thought to play a pivotal role in this process, but the molecular basis of this control is poorly understood. This work characterizes ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 (VvABF2), a grape basic leucine zipper transcription factor belonging to a phylogenetic subgroup previously shown to be involved in ABA and abiotic stress signaling in other plant species. VvABF2 transcripts mainly accumulated in the berry, from the onset of ripening to the harvesting stage, and were up-regulated by ABA. Microarray analysis of transgenic grape cells overexpressing VvABF2 showed that this transcription factor up-regulates and/or modifies existing networks related to ABA responses. In addition, grape cells overexpressing VvABF2 exhibited enhanced responses to ABA treatment compared with control cells. Among the VvABF2-mediated responses highlighted in this study, the synthesis of phenolic compounds and cell wall softening were the most strongly affected. VvABF2 overexpression strongly increased the accumulation of stilbenes that play a role in plant defense and human health (resveratrol and piceid). In addition, the firmness of fruits from tomato (Solanum lycopersicum) plants overexpressing VvABF2 was strongly reduced. These data indicate that VvABF2 is an important transcriptional regulator of ABA-dependent grape berry ripening.

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“…But the roles of several of these transporters remain under-studied in tomato fruit ( figure 2). There is now evidence for the regulation of plant sugar transporters by endogenous sugar levels via kinases [78] which adds an interesting layer of complexity to delineating their role in carbohydrate accumulation in the fruit.…”

Section: Understanding the Role Of Fruit Metabolism In Determining Frmentioning

confidence: 99%

Biochemical factors contributing to tomato fruit sugar content: a review

Beckles¹,

Hong²,

Stamova³

et al. 2011

Fruits

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-Introduction. Consumers and processors value tomatoes with high fruit sugar content; however, most breeding and cultural practices negatively impact this trait. Wild tomato species can accumulate two-to three-fold more fruit sugar than cultivars and are proving to be valuable both as a source of high-sugar loci to broaden the genetic base of currently produced cultivars, and as research material to understand this trait. Synthesis. While cutting-edge genomic approaches have taught us much about fruit phenotypes, it is still important to assess fruit enzyme activities and metabolic fluxes in lines with contrasting fruit sugar accumulation. These metabolic functions are closest to the ripe fruit sugar trait. In this review, we focus our attention on the biochemical pathways, especially starch biosynthesis, that may influence tomato fruit sugars. We try where possible to put this information into a physiological context because together they influence yield. We compare and contrast sugar metabolism in cultivars and wild tomato species and identify factors that may influence differences in their fruit size. Conclusion. Although difficult, we show that it is possible to develop fruit with high horticultural yield and use the breeding line 'Solara' as an example. In addition, we suggest avenues of further investigation to understand the regulation and control of fruit carbohydrate content. USA / Solanum lycopersicum / fruits / sugars / carbohydrate metabolism / carbohydrate contentFacteurs biochimiques contribuant à la teneur en sucre des fruits de tomate : une revue.Résumé -Introduction. Les consommateurs et les industriels apprécient les tomates avec un fort taux en sucres, mais la plupart des pratiques culturales et d'amélioration ont un impact négatif sur ce caractère. Les espèces de tomate sauvage peuvent accumuler 2 ou 3 fois plus de sucres dans le fruit que des cultivars et elles s'avèrent précieuses à la fois comme une source de loci à haute teneur en sucres pour élargir la base génétique des cultivars actuellement produits, et comme matériel de recherche pour comprendre ce caractère. Synthèse. Alors que les approches génomiques de pointe nous ont appris beaucoup sur le phénotype des fruits, il reste important d'évaluer l'activité des enzymes de fruits et les flux métaboliques dans des lignées présentant des situations contrastées d'accumulation de sucres dans les fruits. Ces fonctions métaboliques sont les plus proches du caractère de teneur en sucres dans le fruit mûr. Dans cette synthèse, nous nous sommes focalisés sur les voies biochimiques, en particulier sur la biosynthèse de l'amidon qui peut influencer les sucres dans le fruit des tomates. Nous essayons autant que possible de mettre cette information dans un contexte physiologique car, ensemble, ils influencent le rendement. Nous comparons et mettons en contraste le métabolisme des sucres dans les cultivars et les espèces sauvages de tomate et nous identifions les facteurs qui peuvent influencer des différences de taille des fruits. Conclusio...

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A Sugar-Inducible Protein Kinase, VvSK1, Regulates Hexose Transport and Sugar Accumulation in Grapevine Cells

Cited by 63 publications

References 79 publications

Ecophysiological, Genetic, and Molecular Causes of Variation in Grape Berry Weight and Composition: A Review

Ecophysiological, Genetic, and Molecular Causes of Variation in Grape Berry Weight and Composition: A Review

The Basic Leucine Zipper Transcription Factor ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 Is an Important Transcriptional Regulator of Abscisic Acid-Dependent Grape Berry Ripening Processes

Biochemical factors contributing to tomato fruit sugar content: a review

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