2014
DOI: 10.4238/2014.november.27.14
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A suite of microsatellite markers for genetic management of captive cracids (Aves, Galliformes)

Abstract: ABSTRACT. Cracids are medium to large frugivorous birds that are endemic to the Neotropics. Because of deforestation and overhunting, many species are threatened. The conservation of several species has relied on captive breeding and reintroduction in the wild, but captive populations may be inbred. Microsatellite tools can permit the construction of genetic pedigrees to reduce inbreeding, but only a few loci are available for this group of birds. Here, we present 10 novel polymorphic microsatellite loci and t… Show more

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Cited by 3 publications
(3 citation statements)
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“…The expected heterozygosity H e was significantly higher than H o in the captive population of Hume's pheasant. Similar cases were also observed in the captive populations of cracids [60] and Black-fronted piping guan (Aburria jacutinga, Spix, 1825) [61], which is suggestive of possible inbreeding owing to the small population size. This result is consistent with the positive F IS value (mean = 0.153 ± 0.179) and the mean r values in the captive population.…”
Section: Plos Onesupporting
confidence: 73%
“…The expected heterozygosity H e was significantly higher than H o in the captive population of Hume's pheasant. Similar cases were also observed in the captive populations of cracids [60] and Black-fronted piping guan (Aburria jacutinga, Spix, 1825) [61], which is suggestive of possible inbreeding owing to the small population size. This result is consistent with the positive F IS value (mean = 0.153 ± 0.179) and the mean r values in the captive population.…”
Section: Plos Onesupporting
confidence: 73%
“…DNA samples were amplified at five unlinked microsatellite loci isolated for Black‐fronted Piping Guan ( Aburria 21, Aburria 22, Aburria 44, Aburria 48, Aburria 105) [Costa et al, ], and four heterologous loci developed for Razor‐billed Curassow, Pauxi tuberosa ( Pauxi 1–4, Pauxi 2–2, Pauxi 2–30, and Pauxi 3–4) [Sousa et al, ]. Details on loci isolation, PCR conditions, linkage disequilibrium, and tests for null alleles are described in Costa et al []. Forward primers were fluorescently labeled, amplification products were analyzed on an ABI 3730 automated sequencer (Applied Biosystems), and alleles were scored based on an internal size standard (500 ROX) using GeneMarker 2.6.0 (SoftGenetics, State College, PA).…”
Section: Methodsmentioning
confidence: 99%
“…Details on loci isolation and characterization, as well as PCR conditions, are described in [38] and [39]. Amplifications were performed using fluorescently labeled primers, and products were run on an automated sequencer (ABI 3730).…”
Section: Methodsmentioning
confidence: 99%