A suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testing

Ivask, Angela; Rõlova, Taisia; Kahru, Anne

doi:10.1186/1472-6750-9-41

Cited by 174 publications

(146 citation statements)

References 73 publications

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“…These results indicated that, when the individual pcadCLuc, pzntRluc, or parsRluc sensors were used with mixed CdPb-As solution, Cd and Pb may mutually interfere their respective measured results and Cd may affect the measured result of As. These findings are consistent with those of previous studies, which also observed nonspecific binding of metals such as Cd and Pb to CadC; Pb, Cd, Hg, and Zn to ZntR; and As and Sb to ArsR [6,7].…”

Section: Resultssupporting

confidence: 83%

“…Numerous microbial whole-cell sensors have been developed to measure the soil bioavailability of toxic elements such as Cd, Pb, As, Hg, and Cr [4,5]. However, almost all the developed whole-cell sensors are nonspecific to a certain metal since the regulatory proteins, such as CadC, ZntR, and ArsR, could concurrently recognize several different metals [6,7]. Nonspecific microbial sensors that respond to multiple metals in a certain environment will tend to overestimate the bioavailability of target metals and environmental risks in a co-polluted environment.…”

Section: Introductionmentioning

confidence: 99%

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Detection of bioavailable cadmium, lead, and arsenic in polluted soil by tailored multiple Escherichia coli whole-cell sensor set

Hou

Wang

et al. 2015

Anal Bioanal Chem

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Microbial whole-cell sensor has been widely used to assess bioavailability and risk of toxic elements, but their environmental use is still limited due to the presence of other interfering pollutants and the nonspecific binding in cells, which leads to inaccurate results. Here, we proposed a strategy combining Escherichia coli sensor set with binary regression models for the specific detection of bioavailable cadmium (Cd), lead (Pb), and arsenic (As) in a co-polluted environment. Initial tests suggested that the sensor set respectively termed pcadCluc, pzntRluc, and parsRluc could be classified into two groups according to their specific response to Cd, Pb, and As: group 1 (pcadCluc and pzntRluc) induced by a Cd-Pb mix and group 2 (parsRluc) induced by a Cd-As mix. Based on the variance in responses of each sensor to mixtures of target elements, three binary linear equations for two sensor groups were set up to calculate the individual concentrations in the mixture solutions. This method was then used to quantify the bioavailable Cd, Pb, and As in soils from a co-polluted mining region and to compare the results with other methods. Results showed that the conventional single target sensor method overestimated the bioavailability of each element, while sensor set was credible for accurate bioavailable Cd, Pb, and As quantification and comparable with the results from inductively coupled plasma mass spectrometry (ICP-MS) analysis. Our method can potentially be extended to cover the specific detection of other bioavailable toxic elements in different environmental settings.

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Section: Resultssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Detection of bioavailable cadmium, lead, and arsenic in polluted soil by tailored multiple Escherichia coli whole-cell sensor set

Hou

Wang

et al. 2015

Anal Bioanal Chem

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“…Later, it was modified by different researchers and adapted for their specific purposes [2][3][4][5][6]. Now, the bacterial bioluminescent assay is a traditional and important biotechnological application of the bioluminescence phenomenon [7][8][9][10][11]. The tested physiological parameter here is luminescence intensity that can be easily measured instrumentally.…”

Section: Introductionmentioning

confidence: 99%

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions.

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“…The bioluminescent bioreporter E. coli strains MC1061 (pmerRlux) (9), which responds to inorganic mercury, and MC1061 (pmerRBlux) (11), which responds to both inorganic and organic mercury, were used. The E. coli MC1061 (pDNlux) strain was used as a non-inducible control (11).…”

Section: Bioavailability Of Mercurymentioning

confidence: 99%

Fish Farming Affects the Abundance and Diversity of the Mercury Resistance Gene merA in Marine Sediments

et al. 2011

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Microbes in sediments below fish farming cages are exposed to a variety of compounds used in aquaculture, such as antifoulants, antimicrobials and mercury. Here, we assessed the abundance of the mercury resistance gene merA in the sediment of four fish farms and four pristine sites using quantitative PCR, and at three fish farms and two pristine sites by constructing clone libraries and sequencing merA amplicons. The abundance of merA was consistently greater at fish farm sites than at pristine sites, and the merA phylotypes were different between fish farm and pristine sites. Mercury concentrations were too low to cause selection pressure and did not differ significantly among samples. The primers designed specifically for this study were capable of detecting two new potential merA clades.

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A suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testing

Cited by 174 publications

References 73 publications

Detection of bioavailable cadmium, lead, and arsenic in polluted soil by tailored multiple Escherichia coli whole-cell sensor set

Detection of bioavailable cadmium, lead, and arsenic in polluted soil by tailored multiple Escherichia coli whole-cell sensor set

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Fish Farming Affects the Abundance and Diversity of the Mercury Resistance Gene merA in Marine Sediments

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