A sulfated glycoside from the preparation “tribestan”

Saponins in Tribulus terrestris – Chemistry and Bioactivity

“…Derivatives of 25R-5a-spirostan-3b-ol,12-one (hecogenin, 18) and 25S-5a-spirostan-3b-ol,12-one ( (34) Kock and Enslin (1958), c Gheorghiu and Ionescu-Matiu (1968a), d Zafar and Aeri (1992), e Kintya et al (1972); f Tomova and Panova (1965), g Tosun et al (1991), h Iskenderov (1970), i Sharma and Narula (1977), j Miles et al (1993), k Perepelitsa and Kintya (1975), l Matschenko et al (1990), m Mahato et al (1981), n Conrad et al (2004).…”

“…In the earlier investigations (Perepelitsa and Kintya, 1975;Mahato et al, 1981;Matschenko et al, 1990) the structures of the sugar moieties of the saponins are determined by identification of the monosaccarides obtained on acid hydrolysis by paper chromatography and gas liquid chromatography, or partial hydrolysis followed by isolation and identification of the prosapogenins. The point of attachment of different sugar units are revealed by permethylation of the saponins followed by hydrolysis or methanolysis and identification of the methylated sugars by paper chromatography or gas liquid chromatography.…”

“…In early studies derivatization was required for using electron impact mass spectra (Tomova et al, 1974). Later, field desorption and fast atom bombardment spectra were employed to analyze derivatized and underivatized saponins, providing evidence about molecular mass and sugar sequence by cleavage of glycosidic bonds (Perepelitsa and Kintya, 1975;Mahato et al, 1981;Matschenko et al, 1990;Wang et al, 1996Wang et al, , 1997Wu et al, 1996;Xu et al, 2000). More recently, electrospray ionization (ESI) in the positive and negative ionization mode has become one of the most effective analytical tools for structural characterization of native saponins (Xu et al, 2000;Cai et al, 2001).…”

“…(1980),b Bedir et al (2002), cMatschenko et al (1990), dCombarieu et al (2003), eKostova et al (2002), fTomova and Gjulemetova (1978a), gGjulemetova et al (1982), hGanzera et al (2001), iPerepelitsa and Kintya (1975), jWang et al (1997), kTomova et al (1981).…”

Inhibition of drug resistance in two cancer cell lines(MDR and A2780CIS) in vitro; and the role of selected single nucleotide polymorphisms in cancer

“…We examined saponins and phenolic components (S1-S11) isolated from T. terrestris and S. excelsa in order to find new resistance modifiers (156)(157)(158)(159)(160)(161)(162)(163). All of these compounds were found to be moderate inhibitors of proliferation and MDR in MDR cells.…”