A super-resolution and transmission electron microscopy correlative approach to study intracellular trafficking of nanoparticles

Andrian, Teodora; Muela, Yolanda; Delgado, Lidia; Albertazzi, Lorenzo; Pujals, Sílvia

doi:10.1039/d3nr02838k

Nanoscale

2023

DOI: 10.1039/d3nr02838k

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A super-resolution and transmission electron microscopy correlative approach to study intracellular trafficking of nanoparticles

Teodora Andrian¹,

Yolanda Muela²,

Lidia Delgado³

et al.

Abstract: Nanoparticles (NPs) are used to encapsulate therapeutic cargos and deliver them specifically to the target site. The intracellular trafficking of NPs dictates the NP-cargo distribution within different cellular compartments, and...

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Cited by 6 publications

References 75 publications

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Internalization and Cellular Fate of Protein Corona‐Coated Nanoparticles by Multimodal Multi‐Scale Microscopy

Galdino,

Rabelo,

Scarpa

et al. 2024

Small

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Upon exposure to biological environments, nanoparticles are rapidly coated with biomolecules, predominantly proteins, which alter their colloidal stability, biodistribution, and cell interactions. Despite extensive efforts to investigate the nanoparticles' fate, only a few studies use high‐resolution characterization methods that allow in‐depth characterization, and the existing methodologies are unable to differentiate particles internalized at the onset of incubation from those taken up toward the end of an incubation period. In this study, these limitations related to incubation disparities are overcame and precisely monitored the spatiotemporal displacement of colloidally stable protein corona‐coated nanoparticles within cells. An unprecedented application of cryogenic X‐ray nanotomography, combined with high‐resolution, super‐resolution, and correlative microscopy techniques, revealed the migration of nanoparticles to the perinuclear region while monitoring the evolution of cellular organelles in fully hydrated cells under near‐native conditions, without the need for contrasting agents. Notably, this tracking indicates the progressive fusion of vesicles carrying the nanoparticles intracellularly. This strategy demonstrates the potential for uncovering the temporal aspects of nanoparticle behavior within cells and can be adaptable to a wide range of nanoparticles and cell types, offering a versatile and powerful tool to follow nanoparticles in cellular environments.

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Internalization and Cellular Fate of Protein Corona‐Coated Nanoparticles by Multimodal Multi‐Scale Microscopy

Galdino,

Rabelo,

Scarpa

et al. 2024

Small

View full text Add to dashboard Cite

show abstract

Quantifying nanoparticle delivery: challenges, tools, and advances

Mata Corral,

Alvarez,

Poon

2024

Current Opinion in Biotechnology

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Single-particle electrophoresis to study the adsorption of polyplexes onto anionic particles: A model system for investigating polyplex-cell interactions

Van Daele,

Amer Cid,

Vereecken

et al. 2023

European Polymer Journal

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A super-resolution and transmission electron microscopy correlative approach to study intracellular trafficking of nanoparticles

Abstract: Nanoparticles (NPs) are used to encapsulate therapeutic cargos and deliver them specifically to the target site. The intracellular trafficking of NPs dictates the NP-cargo distribution within different cellular compartments, and...

Cited by 6 publications

References 75 publications

Internalization and Cellular Fate of Protein Corona‐Coated Nanoparticles by Multimodal Multi‐Scale Microscopy

Internalization and Cellular Fate of Protein Corona‐Coated Nanoparticles by Multimodal Multi‐Scale Microscopy

Quantifying nanoparticle delivery: challenges, tools, and advances

Single-particle electrophoresis to study the adsorption of polyplexes onto anionic particles: A model system for investigating polyplex-cell interactions

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