A Superhydrophobic Chip Integrated With an Array of Medium Reservoirs for Long-Term Hanging Drop Spheroid Culture

Sun, Bangyong; Zhao, Yi; Wu, Weimin; Zhao, Qiang; Li, Gang

doi:10.2139/ssrn.3821949

Cited by 2 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different from the works above, Sun et al [85] proposed a medium-reservoir-integrated superhydrophobic (MRI-SH) substrate to develop a new hanging drop (HD) platform. With this approach, they propose a surface that can grow spheroids over a long period of time.…”

Section: Seo Et Al In 2014mentioning

confidence: 99%

“…The laserablated PDMS surface with its micro-and nano-hierarchical roughness combined with the low surface energy of PDMS, shows good superhydrophobic performance (with contact angle up to 156°). On SH surface, a wettable spot array keeps the drops suspended [85].…”

Section: Seo Et Al In 2014mentioning

confidence: 99%

“…Recent works in the literature have demonstrated the ability to produce 3D spheroids using super liquid repellent surfaces. Cell lines, originating from breast [79,81,87,88], carcinogenic stem-cell like [44,81], cervical [79,81,88], colon [79], hepatocellular [78,85,86], ovarian [44] and prostate [89], but also non-tumor cell lines like adipose stem [76,99,100], fibroblasts [84,99], osteoblasts [84,89,99] and umbilical vein endothelial [99] in either monoculture and co-culture conditions, demonstrating a homogeneous cellular distribution in 3D through the live/dead assay. Further studies concerning the morphological and phenotypical characterization [87] and immunofluorescence staining on cells growing in the 3D environment [44] have been carried out.…”

Section: Microenvironment and Integrity Of 3d Spheroidsmentioning

confidence: 99%

“…A significant weakness of the hanging drop method is the limited volume of the droplets and the intrinsic difficulty in exchanging medium of them, causing fast nutrient depletion and the accumulation of metabolic substances. Recently, Sun et al [85] have developed a hanging drop device integrated with medium reservoirs to preserve consistent culture media conditions for long-term spheroid culture. Using numerical models and experimental setups, the nutrient depletion and metabolic waste accumulation rate in the culture medium have been evaluated in both (conventional superhydrophobic) C-SH and medium-reservoirintegrated superhydrophobic (MRI-SH HD) devices.…”

Section: Microenvironment and Integrity Of 3d Spheroidsmentioning

confidence: 99%

See 3 more Smart Citations

Super Liquid-repellent Surfaces and 3D Spheroids Growth

Ferrari¹,

Cirisano²,

Morán³

2022

Front. Biosci. (Landmark Ed)

View full text Add to dashboard Cite

Substrates composition and surface features of materials rule adhesion control of cells to surfaces. As a result, most of the aspects of cell functions, such as spreading, migration, proliferation, and differentiation, can be significantly influenced in biomedical applications. Cell cultures make possible to understand cell biology, tissue morphology, mechanisms of diseases, drug action, and tissue engineering development, among others. Recent techniques related to culturing 3D cell aggregates in the presence of very low wettable surfaces represent an innovative field for in vitro experimentation aimed at more reliable conditions to investigate both tumor and non-tumor cell lines. Matching in particular cell biology to innovative materials, this work reviews the recent literature available on promoting cell aggregates formation strongly influenced by the high surface hydrophobicity. In particular, for spheroid formation, the highest water repellent coatings seem to be required for the significant effectiveness of the process. In this way, 3D cell culture has become a reliable method for reproducing in vitro cellular growth in more realistic physiological conditions.

show abstract

Section: Seo Et Al In 2014mentioning

confidence: 99%

Section: Seo Et Al In 2014mentioning

confidence: 99%

Section: Microenvironment and Integrity Of 3d Spheroidsmentioning

confidence: 99%

Section: Microenvironment and Integrity Of 3d Spheroidsmentioning

confidence: 99%

See 2 more Smart Citations

Super Liquid-repellent Surfaces and 3D Spheroids Growth

Ferrari¹,

Cirisano²,

Morán³

2022

Front. Biosci. (Landmark Ed)

View full text Add to dashboard Cite

show abstract

“…They include microfluidic devices, polymeric hard scaffolds, hydrogel scaffolds, microplates, micropatterned surfaces, and spheroids. Scaffold-free techniques rely on the self-aggregation of cells in specialized culture plates, such as hanging drop microplates 23 , low adhesion plates with ultra-low attachment coating that promotes the spheroid formation 24, 25 , and micropatterned plates that allow for microfluidic cell culture 26 . The development of spheroids is the most popular and convenient technique for 3D cell co-culture.…”

Section: Introductionmentioning

confidence: 99%

3D co-culture of macrophages and fibroblasts in a sessile drop chip for unveiling the role of macrophages in skin wound-healing

Lyu

Cui

Zhou

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Three-dimension (3D) cell co-cultural spheroids exhibit enhanced cellular functions and they can mirror in-vivo microenvironments. Herein, a sessile drop chip was developed to construct 3D spheroids for mirroring the wound healing microenvironment. The sessile drop chip holds the superhydrophobic surface of each microwell which can facilitate cell suspensions transfer to spheroids through the offset of surface tension and gravity, and each microwell has a cylinder hole that offers adequate oxygen to spheroids. It was demonstrated that the 3T3 fibroblast spheroid and the 3T3 fibroblast/M2-type macrophage co-culture spheroid can be formed and remained the physiological activity within nine days. 3D morphology of spheroids was reconstructed using the transparent processing technology and Z-stack function of confocal microscopy. Characteristics of proliferation and differentiation were analyzed by using nano antibody-based 3D immunostaining assay. Results revealed that M2-type macrophages can promote the proliferation and differentiation of the 3T3 fibroblast spheroid. This study presented a novel affordable platform for developing 3D spheroids and provides a 3D model for investigating the macrophages-associated wound healing microenvironment.

show abstract

A Superhydrophobic Chip Integrated With an Array of Medium Reservoirs for Long-Term Hanging Drop Spheroid Culture

Cited by 2 publications

References 0 publications

Super Liquid-repellent Surfaces and 3D Spheroids Growth

Super Liquid-repellent Surfaces and 3D Spheroids Growth

3D co-culture of macrophages and fibroblasts in a sessile drop chip for unveiling the role of macrophages in skin wound-healing

Contact Info

Product

Resources

About