A Supramolecular System for the Electrochemically Controlled Release of Cells

An, Qi; Brinkmann, Jenny; Huskens, Jurriaan; Krabbenborg, Sven O.; Boer, Jan de; Jonkheijm, Pascal

doi:10.1002/ange.201205651

Cited by 31 publications

(8 citation statements)

References 90 publications

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“…31 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 5 cations with micromolar affinities. 34 29,38,39 Furthermore, we have also demonstrated the ability to dynamically display bioactive ligands for cell adhesion and triggered release using electrochemical stimuli. 29,40 These studies indicate the potential of CB[8] for developing highly dynamic and responsive smart surfaces for biomedical applications and research.…”

mentioning

confidence: 97%

Supramolecular Surface Immobilization of Knottin Derivatives for Dynamic Display of High Affinity Binders

Sankaran

Ruiter²,

Cornelissen³

et al. 2015

Bioconjugate Chem.

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Knottins are known as a robust and versatile class of miniprotein scaffolds for the presentation of high-affinity binding peptides; however, to date their application in biomaterials, biological coatings, and surface applications have not been explored. We have developed a strategy to recombinantly synthesize a β-trypsin inhibitory knottin with supramolecular guest tags that enable it to adhere to self-assembled monolayers of the supramolecular host cucurbit[8]uril (CB[8]). We have described a strategy to easily express knottins in E. coli by conjugating them to a fluorescent protein after which they are cleaved and purified. Knottin constructs that varied in the number and position of the supramolecular tag at either the N- or C-termini or at both ends have been verified for their trypsin inhibitory function and CB[8]-binding properties in solution and on surfaces. All of the knottin constructs showed strong inhibition of trypsin with inhibition constants between 10 and 30 nM. Using microscale thermophoresis, we determined that the supramolecular guest tags on the knottins bind CB[8] with a Kd of ∼6 μM in solution. At the surface, strong divalent binding has been determined with a Kd of 0.75 μM in the case of the knottin with two supramolecular guest tags, whereas only weak monovalent binding occurred when only one guest tag was present. We also show successful supramolecular surface immobilization of the knottin using CB[8] and prove that they can be used to immobilize β-trypsin at the surface.

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mentioning

confidence: 97%

Supramolecular Surface Immobilization of Knottin Derivatives for Dynamic Display of High Affinity Binders

Sankaran

Ruiter²,

Cornelissen³

et al. 2015

Bioconjugate Chem.

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“…Their drugencapsulation properties and the easy implementation of targeting ligands make these aggregates promising candidates as site-selective biomedical delivery vectors. [1a,c] Although a variety of external controls have been used for the triggered release of proteins and cells from surfaces, [6] RNA from micelles, [7] and DNA from hydrogels [8] and electrostatic delivery vectors, [9] only chemical reduction [10] and a magnetic field [11] have been used as specific triggers for the release of drugs encapsulated in SNPs.…”

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confidence: 99%

Dual Stimuli‐Responsive Self‐Assembled Supramolecular Nanoparticles

et al. 2014

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Supramolecular nanoparticles (SNPs) encompass multiple copies of different building blocks brought together by specific noncovalent interactions. The inherently multivalent nature of these systems allows control of their size as well as their assembly and disassembly, thus promising potential as biomedical delivery vehicles. Here, dual responsive SNPs have been based on the ternary host-guest complexation between cucurbit[8]uril (CB[8]), a methyl viologen (MV) polymer, and mono- and multivalent azobenzene (Azo) functionalized molecules. UV switching of the Azo groups led to fast disruption of the ternary complexes, but to a relatively slow disintegration of the SNPs. Alternating UV and Vis photoisomerization of the Azo groups led to fully reversible SNP disassembly and reassembly. SNPs were only formed with the Azo moieties in the trans and the MV units in the oxidized states, respectively, thus constituting a supramolecular AND logic gate.

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“…Conversely, in other work, a cyclic RGD cue inactivated by a photolabile ester was covalently attached to silica surfaces to induce spreading upon irradiation 11. There is also an example in the literature describing dynamic signaling through the use of electrochemical stimuli 12. However, it remains a challenge to control the bioactive cues displayed on an ECM mimic in a dynamic and minimally invasive fashion.…”

Section: Summary Of Fibroblast Morphology On Alginate‐cd Surface In Tmentioning

confidence: 99%

Dynamic Display of Bioactivity through Host–Guest Chemistry

et al. 2013

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A molekularer Wirt, β‐Cyclodextrin (CD), der kovalent an eine Alginatmatrix gebunden ist, ermöglicht die dynamische Steuerung eines zellulären Signaldisplays durch Austausch von Gastmolekülen. Die Zugabe löslicher RGDS‐Gäste zum Kulturmedium bewirkt eine induzierte fokale Adhäsion und Spreizung von 3T3‐Fibroblasten auf der normalerweise nicht‐spreizenden Alginatoberfläche. Die Spreizung lässt sich durch Zugabe kompetitiver Gäste mit der mutierten, nicht‐bioaktiven Sequenz RGES umkehren.

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A Supramolecular System for the Electrochemically Controlled Release of Cells

Cited by 31 publications

References 90 publications

Supramolecular Surface Immobilization of Knottin Derivatives for Dynamic Display of High Affinity Binders

Supramolecular Surface Immobilization of Knottin Derivatives for Dynamic Display of High Affinity Binders

Dual Stimuli‐Responsive Self‐Assembled Supramolecular Nanoparticles

Dynamic Display of Bioactivity through Host–Guest Chemistry

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