A surface-display biohybrid approach to light-driven hydrogen production in air

Wei, Wei; Sun, Peiqing; Li, Zhen; Song, Kuisong; Su, Wenyin; Wang, Bao; Liu, Yangzhong; Zhao, Jing

doi:10.1126/sciadv.aap9253

Cited by 159 publications

(124 citation statements)

References 31 publications

(54 reference statements)

Supporting

Mentioning

121

Contrasting

Unclassified

Order By: Relevance

“…In the context of inorganic-biological hybrids, autotrophic bacteria have been investigated intensively, with a focus on simple organic molecules (7–14). Interfacing heterotrophic organisms with light-harvesting inorganics may provide advantages, such as increased efficiency in the production of high-value chemicals (15–17).…”

mentioning

confidence: 99%

Light-driven fine chemical production in yeast biohybrids

Guo

Suástegui

Sakimoto

et al. 2018

Science

321

256

View full text Add to dashboard Cite

Inorganic-biological hybrid systems have potential to be sustainable, efficient, and versatile chemical synthesis platforms by integrating the light-harvesting properties of semiconductors with the synthetic potential of biological cells. We have developed a modular bioinorganic hybrid platform that consists of highly efficient light-harvesting indium phosphide nanoparticles and genetically engineered Saccharomyces cerevisiae: a workhorse microorganism in biomanufacturing. The yeast harvests photo-generated electrons from the illuminated nanoparticles and uses them for the cytosolic regeneration of redox cofactors. This enables the decoupling of biosynthesis and cofactor regeneration, facilitating a carbon- and energy-efficient production of the metabolite shikimic acid – a common precursor for several drugs and fine chemicals. Our work provides a platform for the rational design of biohybrids for efficient biomanufacturing processes with higher complexity and functionality.

show abstract

mentioning

confidence: 99%

Light-driven fine chemical production in yeast biohybrids

Guo

Suástegui

Sakimoto

et al. 2018

Science

321

256

View full text Add to dashboard Cite

show abstract

“…In this work, individual Saccharomyces cerevisiae cells were coated using in situ mineralization generated calcium mineral layer, following the LbL formation of functional polymers acting as a media . Subsequently, another good work using silica as the coating was reported by Choi's group, with PDADMAC as the outermost layer, which can be helpful in the chemically catalytic reaction in the formation of silica layer, under physiologically mild conditions, and this method was utilized for the construction of a bioinorganic hybrid system for photocatalytic hydrogen production . Similarly, Tang and coworkers also explored the silicon dioxide coating, using PDADMAC/PSS and silicon oxide particles alternatively.…”

Section: Strategies For Single‐cell Coatingmentioning

confidence: 96%

Single‐Cell Nanometric Coating Towards Whole‐Cell‐Based Biodevices and Biosensors

Dai

Wang

et al. 2018

ChemistrySelect

View full text Add to dashboard Cite

Living cells with different coatings, like polyelectrolytes, biomolecules, metal nanoparticles or oxides, have more functions compared with the original state, which would be beneficial to many potential applications. Amongst these applications, whole‐cell biosensors/devices are commonly used for high‐throughput disease diagnosis, detection of toxicities, biomedical imaging, environmental monitoring or microbial fuel cells. As a key factor of the fabrication of whole cell biosensors/devices, the techniques for cell immobilization and cell viability have obvious influence on the sensitivity, stability and reproducibility. Cell coatings not only can be helpful to maintain the cell viability, but also can offer multifunction to facilitate the cell immobilization or orientation in the construction of biosensors/devices. Therefore, in this review, we are going to discuss various coating methods and materials, with the focus on their applications in the whole‐cell based biosensors and bio‐devices.

show abstract

“…This strategy was successfully applied to engineer E. coli cells that synthesizes a metal ion complex-binding protein on their surface that collects the light energy in addition to a hydrogenase that uses the solar energy for hydrogen production [15]. Moreover, the additional encapsulation of hydrogen-producing bacteria within a biomimetic silica matrix allowed us to use O 2 -sensitive hydrogenases, even under aerobic conditions [15]. However, compared to the activity of purified hydrogenases, the hydrogen yield that can be obtained from these bioinorganic hybrid systems is very low (0.5 µmol H 2 /10 8 cells within 36 h) [15], and it requires the use of hazardous heavy metal ion complexes.…”

Section: Biohydrogen Production Through Heterologous Gene Expressionmentioning

confidence: 99%

“…In recent decades, hydrogenases have been widely explored for their potential application in biotechnology [12,13]. Hydrogenases can be applied for the construction of hydrogenase-photosystem hybrid complexes for the evolution of H 2 [14,15]. Apart from that, the H 2 oxidation activity of hydrogenases has been exploited in electrochemical fuel cells, which are independent of the traditional platinum catalysts [16].…”

Section: Introductionmentioning

confidence: 99%

Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview

Fan

Neubauer

Lenz

et al. 2020

IJMS

View full text Add to dashboard Cite

Hydrogenases are complex metalloenzymes, showing tremendous potential as H2-converting redox catalysts for application in light-driven H2 production, enzymatic fuel cells and H2-driven cofactor regeneration. They catalyze the reversible oxidation of hydrogen into protons and electrons. The apo-enzymes are not active unless they are modified by a complicated post-translational maturation process that is responsible for the assembly and incorporation of the complex metal center. The catalytic center is usually easily inactivated by oxidation, and the separation and purification of the active protein is challenging. The understanding of the catalytic mechanisms progresses slowly, since the purification of the enzymes from their native hosts is often difficult, and in some case impossible. Over the past decades, only a limited number of studies report the homologous or heterologous production of high yields of hydrogenase. In this review, we emphasize recent discoveries that have greatly improved our understanding of microbial hydrogenases. We compare various heterologous hydrogenase production systems as well as in vitro hydrogenase maturation systems and discuss their perspectives for enhanced biohydrogen production. Additionally, activities of hydrogenases isolated from either recombinant organisms or in vivo/in vitro maturation approaches were systematically compared, and future perspectives for this research area are discussed.

show abstract

A surface-display biohybrid approach to light-driven hydrogen production in air

Abstract: A bioinorganic hybrid system based on bacterial surface display and biomimetic silicification for hydrogen production.

Cited by 159 publications

References 31 publications

Light-driven fine chemical production in yeast biohybrids

Light-driven fine chemical production in yeast biohybrids

Single‐Cell Nanometric Coating Towards Whole‐Cell‐Based Biodevices and Biosensors

Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview

Contact Info

Product

Resources

About