A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells

Ge, Jing; Huang, Yishu; Hu, Xiaoru; Zhong, Jun

doi:10.1016/j.jbiotec.2007.01.037

Cited by 18 publications

(13 citation statements)

References 30 publications

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“…The percentage of cells expressing the reporter gene (egfp) was 2 times higher in A549 cells transduced by HA-displaying virus than A549 cells transduced by the control virus (vAc-EGFP). As expected, HA proteins were efficiently expressed in mammalian cells transduced by vAc-HA-DUAL but not by vAc-HA [19][20][21] .…”

Section: Discussionsupporting

confidence: 78%

Characterization of the immune responses elicited by baculovirus-based vector vaccines against influenza virus hemagglutinin

Yin

Zhang

et al. 2012

Acta Pharmacol Sin

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Aim:To compare the specific immune responses elicited by different baculovirus vectors in immunized mice. Methods: We constructed and characterized two recombinant baculoviruses carrying the expression cassette for the H5N1 influenza virus hemagglutinin (HA) gene driven by either an insect cell promoter (vAc-HA) or a dual-promoter active both in insect and mammalian cells (vAc-HA-DUAL). Virus without the HA gene (vAc-EGFP) was used as a control. These viruses were used to immunize mice subcutaneously and intraperitoneally. The production of total and specific antibodies was determined by ELISA and competitive ELISA. Cytokine production by the spleen cells of immunized mice was studied using the ELISPOT assay. Results: Both the vAc-HA and vAc-HA-DUAL vectors expressed HA proteins in insect Sf9 cells, and HA antigen was present in progeny virions. The vAc-HA-DUAL vector also mediated HA expression in virus-transduced mammalian cell lines (BHK and A547). Both vAc-HA and vAc-HA-DUAL exhibited higher transduction efficiencies than vAc-EGFP in mammalian cells, as shown by the expression of the reporter gene egfp. Additionally, both vAc-HA and vAc-HA-DUAL induced high levels of HA-specific antibody production in immunized mice; vAc-HA-DUAL was more efficient in inducing IFN-γ and IL-2 upon stimulation with specific antigen, whereas vAc-HA was more efficient in inducing IL-4 and IL-6. Conclusion: Baculovirus vectors elicited efficient, specific immune responses in immunized mice. The vector displaying the HA antigen on the virion surface (vAc-HA) elicited a Th2-biased immune response, whereas the vector displaying HA and mediating HA expression in the cell (vAc-HA-DUAL) elicited a Th1-biased immune response.

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Section: Discussionsupporting

confidence: 78%

Characterization of the immune responses elicited by baculovirus-based vector vaccines against influenza virus hemagglutinin

Yin

Zhang

et al. 2012

Acta Pharmacol Sin

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“…This effect is reduced in stem cells and therefore, BV derived vectors are primarily useful in regenerative medicine. Several improvements of this vector system were achieved by altering the viral surface protein gp64 with different signal peptides for transduction of certain mammalian cells [50]. Other improvements could be achieved by protecting BV vectors from inhibition factors within serum which lead to more efficient transduction in vitro and in vivo [51].…”

Section: Viral Vectors For Talen Deliverymentioning

confidence: 99%

Progress and Problems with Viral Vectors for Delivery of Talens

Bergmann¹

2014

J Mol Genet Med

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Transcription Activator-Like Effector Nucleases (TALENS) and Their Mode of ActionFor sequence-specific genome engineering and its biotechnological and medical applications various systems were tested. Most recent tools include designer nucleases and the bacteria derived clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system using short RNA to induce precise cleavage at endogenous genomic loci [1,2]. Designer nucleases are mainly represented by zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) [3][4][5][6][7], combining features of a customized DNA binding motif for sequence-specific DNA binding domain and a nuclease for introduction of doubled-strand DNA (dsDNA) breaks at the target site.TALENs are a promising new class of designer nucleases that can be specifically designed to bind DNA sequences of interest and to introduce dsDNA breaks. They are chimeric proteins consisting of a N-terminal nuclear localization signal, a central DNA binding domain and a C-terminal FokI nuclease domain. The DNA binding domain originates from transcription activator like effectors (TALEs) of the bacterial plant pathogen Xanthomonas, used by this bacterium to alter the expression of several host genes [8]. Its main characteristic is a central repeat region consisting of a variable number of incomplete tandem repeats that are usually comprised of 33-35 amino acids (aa) that are identical except for the hypervariable repeat-variable diresidue (RVD) at a positions 12 and 13 [6]. With some degree of degeneracy, the RVD of each repeat is specific for binding a corresponding nucleotide in their contiguous target DNA sequence [6,8]. The C-terminal FokI domain provides non-specific endonuclease activity and similar to ZFNs, binding of the TALE domains upstream and downstream of the respective DNA target and subsequent dimerization at the FokI nuclease domain lead to dsDNA breaks. It is of note that the spacer between the TALEN DNA binding sites needs to be considered when designing novel TALENs [6,7]. After binding, dsDNA breaks are introduced which can than activate cellular pathways either leading to homologous recombination in the presence of the respective homologous donor AbstractIt has long been envisaged that gene disruption or gene correction in affected target cells can be efficiently conducted in vitro and in vivo and over the recent years several tools for achieving this goal were developed. Designer nucleases such as zinc finger nucleases (ZFNs) were extensively explored and more recently transcription activator-like effector nucleases (TALENs) were introduced for sequence-specific genome engineering in the mammalian genome. ZFNs and TALENs are fusion proteins containing a customized DNA-binding motif for sequence-specific DNA binding linked to a nuclease for introduction of double-stranded DNA breaks. Both systems were explored in mammalian cells using non-viral and viral delivery methods. Herein, we will provide a state-ofthe-art overview of available virus-base...

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“…For instance, baculovirus poorly transduces B lymphocytes (Cheng et al, 2004;Condreay et al, 1999). Via gp64 fusion, the transduction has been enhanced by displaying the short peptide motif from gp350/220 of Epstein-Barr virus (EBV, which naturally infects B cells) on the baculovirus envelope (Ge et al, 2007). Alternatively, the cytoplasmic transduction peptide (CTP) has been fused to gp64 to enhance the baculovirus transduction of Vero E6, U-2OS and CHO-RD cells (Chen et al, 2011b).…”

Section: Surface Display Via Gp64 Fusion or Heterologous Proteinmentioning

confidence: 99%

Baculovirus as a gene delivery vector: Recent understandings of molecular alterations in transduced cells and latest applications

Chen

Lin

Chen

et al. 2011

Biotechnology Advances

128

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Baculovirus infects insects in nature and is non-pathogenic to humans, but can transduce a broad range of mammalian and avian cells. Thanks to the biosafety, large cloning capacity, low cytotoxicity and non-replication nature in the transduced cells as well as the ease of manipulation and production, baculovirus has gained explosive popularity as a gene delivery vector for a wide variety of applications. This article extensively reviews the recent understandings of the molecular mechanisms pertinent to baculovirus entry and cellular responses, and covers the latest advances in the vector improvements and applications, with special emphasis on antiviral therapy, cancer therapy, regenerative medicine and vaccine.

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A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells

Cited by 18 publications

References 30 publications

Characterization of the immune responses elicited by baculovirus-based vector vaccines against influenza virus hemagglutinin

Characterization of the immune responses elicited by baculovirus-based vector vaccines against influenza virus hemagglutinin

Progress and Problems with Viral Vectors for Delivery of Talens

Baculovirus as a gene delivery vector: Recent understandings of molecular alterations in transduced cells and latest applications

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