A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles

Zhang, Yinan; Chen, Yu; Yu, Jun; Birch, David J. S.

doi:10.15761/mdde.1000119

Cited by 6 publications

(5 citation statements)

References 45 publications

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“…Generally, the lifetime of fluorescence of many endogenous fluorophores is shorter, thereby providing bio-objects autofluorescence or for dyes used in fluorescence imaging. This provides potential for lifetime imaging (FLIM) [23,24] and FLIM monitoring for the Föster resonance energy transfer effect between gold nanoparticles and fluorescent molecules in biosensing applications [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Perevedentseva

Ali

Lin

et al. 2020

Biomed. Opt. Express

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In the present work, we report the imaging of Au nanostars nanoparticles (AuNSt) and their multifunctional applications in biomedical research and theranostics applications. Their optical and spectroscopic properties are considered for the multimodal imaging purpose. The AuNSt are prepared by the seed-meditated method and characterized for use as an agent for bio-imaging. To demonstrate imaging with AuNSt, penetration and localization in different biological models such as cancer cell culture (A549 lung carcinoma cell), 3D tissue model (multicellular tumor spheroid on the base of human oral squamous carcinoma cell, SAS) and murine skin tissue are studied. AuNSt were visualized using fluorescence lifetime imaging (FLIM) at two-photon excitation with a pulse duration 140 fs, repetition rate 80 MHz and 780 nm wavelength femtosecond laser. Strong emission of AuNSt at two-photon excitation in the near infrared range and fluorescence lifetime less than 0.5 ns were observed. It allows using AuNSt as a fluorescent marker at two-photon fluorescence microscopy and lifetime imaging (FLIM). It was shown that AuNSt can be observed inside a thick sample (tissue and its model). This is the first demonstration using AuNSt as an imaging agent for FLIM at two-photon excitation in biosystems. Increased scattering of near-infrared light upon excitation of AuNSt surface plasmon oscillation was also observed and rendered using a possible contrast agent for optical coherence tomography (OCT). AuNSt detection in a biological system using FLIM is compared with OCT on the model of AuNSt penetrating into animal skin. The AuNSt application for multimodal imaging is discussed.

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Section: Introductionmentioning

confidence: 99%

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Perevedentseva

Ali

Lin

et al. 2020

Biomed. Opt. Express

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“…7−13 These combined with low toxicity, water solubility, and biocompatibility, have made gold nanorods a versatile nanomaterial with demonstrated applications in various areas including biological sensing, imaging, drug delivery, and cancer therapy. 14−20…”

Section: Introductionmentioning

confidence: 99%

“…Gold nanorods have been demonstrated as promising agents for various biomedical applications including cancer diagnosis and treatment. − The choice of gold nanorods stems from their unique optical properties arising from localized surface plasmon resonance. , The longitudinal surface plasmon mode of gold nanorods depends on the particle aspect ratio − and can thus be tuned from the visible to the near infrared (NIR) region. This shape-dependent optoelectronic property manifests itself in various processes such as strong surface-enhanced Raman scattering, two-photon luminescence, surface plasmon enhanced energy transfer, and photothermal effects. − These combined with low toxicity, water solubility, and biocompatibility, have made gold nanorods a versatile nanomaterial with demonstrated applications in various areas including biological sensing, imaging, drug delivery, and cancer therapy. − …”

Section: Introductionmentioning

confidence: 99%

Synthesis of Small Gold Nanorods and Their Subsequent Functionalization with Hairpin Single Stranded DNA

et al. 2019

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Small gold nanorods have a significantly large absorption/scattering ratio and are especially beneficial in exploiting photothermal effects, for example in photothermal therapy and remote drug release. This work systematically investigates the influence of growth conditions on the size, growth yield, and stability of small gold nanorods. The silver-assisted seed-mediated growth method was optimized to synthesize stable small gold nanorods with a high growth yield (>85%). Further study on the influence of silver ions on the growth facilitates the growth of small gold nanorods with tunable longitudinal surface plasmon resonance from 613 to 912 nm, with average dimensions of 13–25 nm in length and 5–6 nm in diameter. Moreover, the small gold nanorods were successfully functionalized with thiol-modified hairpin oligonucleotides (hpDNA) labeled with Cy5. Fluorescence intensity measurements show an increase in the presence of target DNA and an enhanced signal/background ratio when the longitudinal surface plasmon resonance of small gold nanorods overlaps with the excitation and emission wavelength of Cy5. This coincides with a reduced fluorescence lifetime of Cy5 in the hairpin structure, indicating surface plasmon resonance-enhanced energy transfer to the small gold nanorods. This study may provide insight on the synthesis and functionalization of small gold nanorods in biomedical sensing and therapy.

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“…The result clearly shows that the fluorescence lifetime of ATTO 647N decreased in the presence of GNRs in both methods. The decrease of fluorescence lifetime in the presence of GNRs is attributed to an energy transfer from donor molecules to acceptor molecules non-radiatively via dipole-dipole interaction between them [20,22,29]. The amount of energy transfers also known as FRET efficiency, Ε, is directly related to lifetime reduction that can be calculated using equation (5).…”

Section: Fret Measurements Through Stimulated Emission and Tcspcmentioning

confidence: 99%

“…For Hetero-FRET, GNRs, attributing to its strong NIR absorption & scattering, have broad and prospective applications including biomedical sensing & imaging, drug delivery, and therapy [18,19]. Recently, Zhang et al [20] reported FLIM-FRET approaches with Au nanorods using conventional TCSPC module. For comparison, we have implemented stimulated emission based pump-probe setup in time-domain to investigate Hetero-FRET and depolarization with much higher temporal resolution.…”

Section: Introductionmentioning

confidence: 99%

High temporal resolution and polarization resolved fluorescence lifetime measurements through stimulated emission

Rehman¹,

Das

Chen³

et al. 2020

Methods Appl. Fluoresc.

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We have implemented polarization-resolved fluorescence lifetime measurement through stimulated emission based pump-probe technique, which promises much higher temporal resolution (∼4 ps) than conventional time-correlated single-photon counting (TCSPC). The depolarization of ATTO 647N fluorescent dye is resolved through anisotropy fluorescence lifetime measurements, with variable time delay introduced between the pump and the probe beams. Importantly, the polarization anisotropy measurement and the corresponding rotational correlation time characterization of the fluorescent dye are carried out at various temperatures. We have also demonstrated the need of high temporal resolution via hetero Förster energy transfer (Hetero-FRET) through the interaction between the gold nanorods (GNRs) and the fluorescent dye ATTO 647N. Notably, our results compare highly favorably with conventional TCSPC method, which is rather limited in temporal resolution, for the above characterization. Additionally, this technique is applicable even under ambient light while being very cost-effective and robust.

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A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles

Cited by 6 publications

References 45 publications

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Synthesis of Small Gold Nanorods and Their Subsequent Functionalization with Hairpin Single Stranded DNA

High temporal resolution and polarization resolved fluorescence lifetime measurements through stimulated emission

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