A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

Dyankov, Georgi; Borisova, Ekaterina; Belina, E.; Kisov, Hristo; Ангелов, Иван; Gisbrecht, Alexander; Strijkova, Velichka; Malinowski, N.

doi:10.3390/s20195572

Cited by 13 publications

(12 citation statements)

References 34 publications

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“…The main idea of the MAPLE technique is that the matrix, in which the ligand is dissolved, absorbs the laser power, so that the decomposition of the recognition molecules is avoided. In a previous publication [ 43 ], we showed that this technique provides the deposition of intact molecules, as well as high-accuracy and -sensitivity detection [ 44 ].…”

Section: Optical Platforms and Functionalization For Virus-sensing Ap...mentioning

confidence: 99%

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Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study

et al. 2023

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The danger of the emergence of new viral diseases and their rapid spread demands apparatuses for continuous rapid monitoring in real time. This requires the creation of new bioanalytical methods that overcome the shortcomings of existing ones and are applicable for point-of-care diagnostics. For this purpose, a variety of biosensors have been developed and tested in proof-of-concept studies, but none of them have been introduced for commercial use so far. Given the importance of the problem, in this study, long-period grating (LPG) and surface plasmon resonance (SPR) biosensors, based on antibody detection, were examined, and their capabilities for SARS-CoV-2 structural proteins detection were established. Supersensitive detections of structural proteins in the order of several femtomoles were achieved by the LPG method, while the SPR method demonstrated a sensitivity of about one hundred femtomoles. The studied biosensors are compatible in sensitivity with ELISA and rapid antigen tests but, in contrast, they are quantitative, which makes them applicable for acute SARS-CoV-2 infection detection, especially during the early stages of viral replication.

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Section: Optical Platforms and Functionalization For Virus-sensing Ap...mentioning

confidence: 99%

“…This concentration was established after many experiments, as the use of MAPLE technology requires a detection sensitivity tradeoff with accuracy. Details regarding the MAPLE technique and the parameters of the immobilization procedure can be found in [ 43 ].…”

Section: Optical Platforms and Functionalization For Virus-sensing Ap...mentioning

confidence: 99%

Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study

et al. 2023

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“…Matrix-assisted laser evaporation (MAPLE) was used for the ligand deposition to avoid build-in matrix and related mediating molecules—the main causes for biosensor non-specific response. Details about the MAPLE technology and Hb/Mb immobilization can be found in [ 19 , 20 ]. Hemoglobin was deposited simultaneously on DR LPG structures (with their sensitivities measured in advance) and on SPR gratings.…”

Section: Methodsmentioning

confidence: 99%

Binding of SARS-CoV-2 Structural Proteins to Hemoglobin and Myoglobin Studied by SPR and DR LPG

Dyankov

Genova-Kalоu

Eftimov

et al. 2023

Sensors

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One of the first clinical observations related to COVID-19 identified hematological dysfunctions. These were explained by theoretical modeling, which predicted that motifs from SARS-CoV-2 structural proteins could bind to porphyrin. At present, there is very little experimental data that could provide reliable information about possible interactions. The surface plasmon resonance (SPR) method and double resonance long period grating (DR LPG) were used to identify the binding of S/N protein and the receptor bind domain (RBD) to hemoglobin (Hb) and myoglobin (Mb). SPR transducers were functionalized with Hb and Mb, while LPG transducers, were only with Hb. Ligands were deposited by the matrix-assisted laser evaporation (MAPLE) method, which guarantees maximum interaction specificity. The experiments carried out showed S/N protein binding to Hb and Mb and RBD binding to Hb. Apart from that, they demonstrated that chemically-inactivated virus-like particles (VLPs) interact with Hb. The binding activity of S/N- and RBD proteins was assessed. It was found that protein binding fully inhibited heme functionality. The registered N protein binding to Hb/Mb is the first experimental fact that supports theoretical predictions. This fact suggests another function of this protein, not only binding RNA. The lower RBD binding activity reveals that other functional groups of S protein participate in the interaction. The high-affinity binding of these proteins to Hb provides an excellent opportunity for assessing the effectiveness of inhibitors targeting S/N proteins.

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“…Matrix-assisted laser evaporation (MAPLE) was used for the bioreceptor deposition to avoid built-in matrix and related mediating molecules -the main causes for biosensor non-specific response. Details about the MAPLE-mediated immobilization can be found in our previously published results [6,7 ].…”

Section: Evaluation Of the H Pylori-le B Systemmentioning

confidence: 99%

Biosensor-based serological assay for diagnosing Helicobacter pylori infection

Kolev,

Hikova,

Kisov

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Helicobacter pylori causes the most common bacterial infection involving 50% of the global population. According to the World Health Organization H. pylori infection contributes to approximately 75% of the stomach cancer cases and 5.5% of all types of cancer. Therefore, timely diagnosis of the infection is highly desirable. Serological assays are widely performed for diagnosing H. pylori infection, the most frequently used one being ELISA. In the present study we showed that a serological assay can also be carried out using a biosensor based on Surface Plasmon Resonance (SPR). Unlike our previous studies where we used amplitude detection of the binding reactions, here we applied phase-sensitive detection. It was performed with a the channeled spectroscopic ellipsometer, which allowed fast measurement with high sensitivity. Thus, the detection limit achieved was more than two times lower than that of the amplitude detection. In terms of CFU, phase detection was sensitive even at 200 CFU, while amplitude detection was applicable at 3000 CFU.

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A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

Cited by 13 publications

References 34 publications

Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study

Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study

Binding of SARS-CoV-2 Structural Proteins to Hemoglobin and Myoglobin Studied by SPR and DR LPG

Biosensor-based serological assay for diagnosing Helicobacter pylori infection

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