A surgical model to study in vivo efflux of cholesterol from porcine aorta Evidence for cholesteryl ester transfer through the aortic wall

Hjelms, E; Nordestgaard, Børge G.; Stender, Steen; Kjeldsen, Knud

doi:10.1016/0021-9150(89)90087-7

Cited by 10 publications

(17 citation statements)

References 17 publications

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“…It is also assumed that neither hydrolysis of labeled esterified cholesterol nor esterification of labeled free cholesterol occurs in the arterial wall during labeling of the isolated aortic segment. The quantitative importance of these conversions was demonstrated in Figure 6 of the previous article 6 by use of plasma labeled with 3 H primarily in esterified cholesterol and with U C primarily in free cholesterol, or vice versa. Based on the results from five animals, it was concluded that labeled free cholesterol was not esterified in the arterial wall and that labeled esterified cholesterol was not hydrolyzed to any large amount during the pulse labeling period.…”

Section: Calculationsmentioning

confidence: 99%

“…To take into account that 3 H-and u C-esterified cholesterol were both present in both HDL and LDL, influx of HDL and LDL cholesteryl ester was calculated with a modification of a previously published method 8 with two linear equations: 6) is negligible compared with the amount of labeled cholesteryl ester that enters the wall during the influx period. In Figure 5 of the previous article, 6 labeling of the arterial wall with labeled total plasma cholesteryl ester was compared for 2 and 4 hours of labeling; the "sink assumption" seemed justified since the relative tissue radioactivity of the total arterial wall after a 2-hour labeling was 51 % of that after a 4-hour labeling. It is also assumed that neither hydrolysis of labeled esterified cholesterol nor esterification of labeled free cholesterol occurs in the arterial wall during labeling of the isolated aortic segment.…”

Section: Calculationsmentioning

confidence: 99%

“…6 The surgical procedure for pulse labeling of arterial tissue in the isolated aortic segment and for the following cold chase in vivo has also been described. 6 Briefly, through a left thoracotomy, 6 to 8 cm of the descending thoracic aorta was dissected free while intercostal arteries and other aortic branches were ligated, leaving the adventitia in situ. After an external shunt of silicone tubing was inserted cranially and caudally to the dissected aortic segment, the segment was crossclamped at each end and thus excluded temporarily from the circulation.…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…These questions were addressed in the present study. We used a previously described animal model, 6 where lipoprotein/arterial wall interactions were studied in normocholesterolemic pigs with nonatherosderotjc aortas. equalvolume of stored erythrocytes.…”

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confidence: 99%

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Different efflux pathways for high and low density lipoproteins from porcine aortic intima.

et al. 1990

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To study the efflux of high (HDL) and low (LDL) density lipoproteins from the arterial wall In vivo, a surgical model in pigs was used. An isolated segment of the lesion-free thoracic aorta was pulse labeled from the lumen of the artery with 3 H-cholesteryl ester labeled HDL and 14 C-cholesteryl ester labeled LDL. Subsequently, the labeled aortic segment was exposed to cold chase In vivo. The transfer of HDL cholesteryl ester from plasma Into intima expressed as intlmal clearance was three to seven times greater than that of LDL cholesteryl ester. At least 50%, but possibly as much as 95%, of the HDL cholesteryl ester that entered the arterial intima during a period of 4 hours penetrated the arterial wall beyond the internal elastic lamina. In contrast, less than 15% of the LDL cholesteryl ester that entered the arterial Intima in the same period penetrated beyond the lumlnal layer. After 24 hours of cold chase In vivo, more than 80% of both labeled HDL esterlfled cholesterol and labeled LDL esterlfled cholesterol had disappeared from the arterial wall. Transmural profiles after 9 hours of cold chase showed that labeled HDL was present throughout the entire arterial wall, whereas labeled LDL In quantitative amounts was present only In the luminal layer. The results suggest that the most Important efflux route for HDL esterlfled cholesterol Is through the vasa vasorum and lymphatics in the outer media and adventltJa, whereas LDL esterlfled cholesterol predominantly leaves Intima via the lumen of the artery. (Arteriosclerosis 10:477-485, May/June 1990)

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Section: Calculationsmentioning

confidence: 99%

Section: Calculationsmentioning

confidence: 99%

Section: Surgical Proceduresmentioning

confidence: 99%

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confidence: 99%

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Different efflux pathways for high and low density lipoproteins from porcine aortic intima.

et al. 1990

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“…Experimental surgical interventions in the pig do not require full aortic transplant. Remarkably, delivery of labeled cholesterol esters, either in the form of LDL or HDL, to a ligated and temporarily bypassed segment of the pig thoracic aorta revealed that HDL passes through the media and enters adventitia efficiently, leading investigators in the late 1980s to conclude that HDL was likely cleared through adventitial lymphatics (70,71). Labeled LDL, by contrast, penetrated only into the intimal layer, indicating specificity in trafficking through the medial wall for labeled HDL.…”

Section: Atherosclerosis and Cholesterol Transport Through Interstitimentioning

confidence: 99%

Lymphatic transport of high-density lipoproteins and chylomicrons

Randolph¹,

Miller²

2014

J. Clin. Invest.

180

158

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The life cycles of VLDLs and most LDLs occur within plasma. By contrast, the role of HDLs in cholesterol transport from cells requires that they readily gain access to and function within interstitial fluid. Studies of lymph derived from skin, connective tissue, and adipose tissue have demonstrated that particles as large as HDLs require transport through lymphatics to return to the bloodstream during reverse cholesterol transport. Targeting HDL for therapeutic purposes will require understanding its biology in the extravascular compartment, within the interstitium and lymph, in health and disease, and we herein review the processes that mediate the transport of HDLs and chylomicrons through the lymphatic vasculature.

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Lymphatic Vessel Network Structure and Physiology

Breslin

Yang

Scallan

et al. 2018

Comprehensive Physiology

274

258

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The lymphatic system is comprised of a network of vessels interrelated with lymphoid tissue, which has the holistic function to maintain the local physiologic environment for every cell in all tissues of the body. The lymphatic system maintains extracellular fluid homeostasis favorable for optimal tissue function, removing substances that arise due to metabolism or cell death, and optimizing immunity against bacteria, viruses, parasites, and other antigens. This article provides a comprehensive review of important findings over the past century along with recent advances in the understanding of the anatomy and physiology of lymphatic vessels, including tissue/organ specificity, development, mechanisms of lymph formation and transport, lymphangiogenesis, and the roles of lymphatics in disease.

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A surgical model to study in vivo efflux of cholesterol from porcine aorta Evidence for cholesteryl ester transfer through the aortic wall

Cited by 10 publications

References 17 publications

Different efflux pathways for high and low density lipoproteins from porcine aortic intima.

Different efflux pathways for high and low density lipoproteins from porcine aortic intima.

Lymphatic transport of high-density lipoproteins and chylomicrons

Lymphatic Vessel Network Structure and Physiology

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