A Survey of Argentine and Lebanese Bone for Salmonellas with Particular Reference to the Isolation of Salmonella typhimurium

Abstract

The incidence of Salmonella typhimurium in imported bone meal may be underestimated by conventional isolation techniques because of the presence of other salmonella serotypes. A method was developed with a serological bias towards recovery of serotypes with ‘i’as H phase 1 antigen. By this technique, 44 strains of S. typhimurium were isolated from Argentinian bone meal compared with nine strains cultured by the unbiased method. The technique was less successful with Lebanese bone. The serological technique was most effective when several salmonella serotypes not possessing the ‘i’antigen were present in a sample. S. kentucky (8, 20:i:z6), like S. typhimurium was also efficiently isolated by the serological method. In 12 specimens both S. typhimurium and S. kentucky were present. The two serotypes were easily separated by examining four 25 g sub‐samples of the specimen.

Συγκριτικη Μελετη Νεων Μεθοδων Απομονωσεως Σαλμονελλων Απο Προιοντα Ζωικης Προελευσεως

“…Γιά νά είναι πλήρη τά δεδομένα του πίνακα αύτοΰ αναφέρουμε δτL από τους44 θετικούς γ uà σαλμονέλλες σωλήνες έπανεμπλουτισμοΰ MK/SMM 18 έγιναν θετικού σέ μύα ήμερα, καύ 26 σε δύο ημέρες, ένώ άπό τους 42 θεϊκούς σωλήνες τοΰ ύλικοΰ RV/SMM 9 έγιναν θε τικού σέ μύα ήμερα, καύ 33 σέ δύο ήμερες. "0 χρόνος αυτός πρα-KTUKa δέν έχει διαφορά συγκρύνοντας τά δύο ύλuκά έπανεμπλουτισμοΰ στην περύπτωση τών μεσεντερύων λεμφογαγγλύων.…”