“…It implies several risks, such as a considerable increase in volatile acidity, microbiological problems associated with delayed MLF (Murat et al, 2007) and formation of undesirable metabolites, such as biogenic amines (Liu, 2002;Marcobal et al, 2006;Polo et al, 2011). Biogenic amines that can affect human health and lead to low quality wines, are mainly formed by decarboxylation of the corresponding amino acids by LAB (Zhijun et al, 2007;Izquierdo et al, 2009). Inoculation with defined starter cultures reduces the potential of spoilage by other LAB, thus ensuring the rapid onset of MLF, better control over the production of aromatic compounds and wine flavour (Bauer & Dicks, 2004) and avoiding the accumulation of biogenic amines (Manfroi et al, 2009). The commercial introduction of yeast cultures for wine inoculation has improved the control of AF, and their use has become popular among winemakers for the prevention of problems associated with spontaneous process in many regions.…”