A survey of herbicides for their effect upon protein synthesis.

Mann, Jay D.; Jordan, L. S.; Day, B. E.

doi:10.1104/pp.40.5.840

Cited by 98 publications

(53 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although microtubules, or microtubule associated material, are implicated as the target of IPC action in all of these studies, the wide variation in concentration of IPC used, the different assay methods, and the variable responses in these widely diverse organisms make it difficult to propose a uniform mode of action for IPC . In addition, earlier reports that the 3-chlorophenyl derivative of IPC, CIPC, inhibits protein synthesis in several plant systems (Mann et al ., 1965 ;1967) and the hypothesis that IPC and CIPC may inhibit messenger RNA transcription and hence, new protein synthesis (Mann et al ., 1967 ;Keitt, 1967) suggested that some of the responses listed above (e .g ., inhibition of cilia regeneration and flagellum morphogenesis) may be due to inhibition of protein synthesis, including the synthesis of microtubule protein .…”

Section: Discussionmentioning

confidence: 98%

MICROTUBULE BIOGENESIS AND CELL SHAPE IN OCHROMONAS

Brown

Bouck

1974

The Journal of Cell Biology

View full text Add to dashboard Cite

The role of microtubules and microtubule nucleating sites in the unicell, Ochromonas has been examined through the use of two mitotic inhibitors, isopropyl N-phenylcarbamate (IPC) and isopropyl N-3-chlorophenyl carbamate (CIPC) . Although IPC and CIPC have little or no effect on intact microtubules, the assembly of three separate sets of microtubules in Ochromonas has been found to be differentially affected by IPC and CIPC . The assembly of flagellar microtubules after mechanical deflagellation is partially inhibited ; the reassembly of rhizoplast microtubules after pressure depolymerization is totally inhibited (however, macrotubules may form at the sites of microtubule initiation or elsewhere) ; and, the reassembly of the beak set of microtubules after pressure depolymerization may be unaffected although similar concentrations of IPC and CICP completely inhibit microtubule regeneration on the rhizoplast . These effects on microtubule assembly, either inhibitory or macrotubule inducing, are fully reversible . The kinetics of inhibition and reversal are found to be generally similar for both flagellar and cell shape regeneration . Incorporation data suggest that neither IPC nor CIPC has significant effects on protein synthesis in short term experiments . Conversely, inhibiting protein synthesis with cycloheximide has little effect on microtubule regeneration when IPC or CIPC is removed . Although the exact target for IPC and CIPC action remains uncertain, the available evidence suggests that the microtubule protein pool or the microtubule nucleating sites are specifically and reversibly affected . Comparative experiments using the mitotic inhibitor colchicine indicate some similarities and differences in its mode of action with respect to that of IPC and CIPC on assembly and disassembly of microtubules in these cells .In the first two papers of this series (Bouck and ently independent sets of cytoplasmic microBrown, 1973 ; Brown and Bouck, 1973) evidence tubules which (a) differ from each other in sensiwas presented that the characteristic asymmetric tivity to the microtubule depolymerizing agents shape of Ochromonas is mediated by two appar-colchicine and hydrostatic pressure, (b) are 514

show abstract

Section: Discussionmentioning

confidence: 98%

MICROTUBULE BIOGENESIS AND CELL SHAPE IN OCHROMONAS

Brown

Bouck

1974

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…Amato et al (1) reported similar responses in corn and cotton. Mann et al (19) found th,at trifluralin (11,12,17 If the vailtues for the sRNA l)eaks (I and I1) are combined and also those of the rRNA (IV, V, VI), )there ,is an increase in the sRNA fractions and a decrea,se in the 'rRNA fraction from 48 to 96 houirs in the control and treated roots. DNA (peak III) shows no change beltween treated and control roots from 48 to 96 hours, wh,ile tthe control shoots have a decreased amounit ait 96 hours and the treated shoots an increased amoSunit.…”

mentioning

confidence: 99%

Effect of Trifluralin on Growth, Morphology, and Nucleic Acid Synthesis

1968

View full text Add to dashboard Cite

Abstract. Roots and shoots of corn seedlings (Zea inays L. var. Dixie 18) germinated in trifluralin (a,a,a-trifluoro-2,6-dinitro-N,N-dipropvl-p-toluidine) Certain growth regulating chemicals such as 'Tordon (4-amino-3,5,6-trichlo.ropi'colinic acid) and 2,4-D prommote radlial enlargement of iplant segments and also affect nucleic acid metabolism (11,12,17). The carbamates also promote radial enlargement of root tissue and are believed to disrupt spindle formiation in cell division (7). Standifer et al. (28) and Talbert (29) found 'that swelling of the root terminalts was the iobvious morphological effect caused by the herbicide trifluralin (a, a, a-trifluoro-2, 6-dini.tro-N, N-dipropyl-p-tolluidine). Talber't (29)

show abstract

“…1 This research was supported by funds from the Massachusetts Agricultural Experiment Station. The literature suggests that many herbicides act upon protein synthesis (7,27,31) and cell membrane permeability (6,26). Dicamba was reported to inhibit leucine incorporation into protein in soybean hypocotyl sections and to inhibit induction of a-amylase in barley aleurone layers (31).…”

mentioning

confidence: 99%

Participation of Ethylene in Common Purslane Response to Dicamba

Stacewicz-Sapuncakis

Marsh²,

Vengris³

et al. 1973

Plant Physiol.

View full text Add to dashboard Cite

The responses of common purslane (Portulaca oleraqea L.) plants to 2-methoxy-3,66-dichlorobenzoic acid (dicamba) were found to be similar in many respects to ethylene fumigation effects. Dicamba and ethylene increased the permeability of celi membranes in purslane tissues. An increased efflux of electrolytes was observed in the bending region of the stems of dicamba-treated plants. Epinastic leaves after dicamba (10 micrograms) and ethylene (microliter per liter) treatments showed an increased efflux of rubidium. The permeability effects were observable within 1 day after dicamba or ethylene application. Protein metabolism in purslane leaves was not influenced by dicamba until 2 days after treatment, as indicated by reduced nitrate reductase activity. Inhibition of phenylalanine-U-YC incorporation into protein was observed 3 days after treatment. Ethylene reduced both phenylalanine-U-"C incorporation into protein and nitrate reductase actvity within 1 day. Dicamba caused a rapid increase in ethylene production in purslane plants to levels many times greater than those observed in untreated plants. It was concluded that the dicamba-enhanced production of ethylene is responsible for many of the observed effects of the herbicide. Dicamba is a systemic herbicide which has been used effectively to control many broad-leaved weeds resistant to other herbicides. Common purslane, a troublesome weed which is extremely drought-resistant, is not easily controlled by 2 ,4-D or 2,4,5-T but is sensitive to dicamba (23,39 The literature suggests that many herbicides act upon protein synthesis (7, 27, 31) and cell membrane permeability (6, 26). Dicamba was reported to inhibit leucine incorporation into protein in soybean hypocotyl sections and to inhibit induction of a-amylase in barley aleurone layers (31). Magalhaes and Ashton (26) found a decrease in the permeability of cell membranes of dicamba-treated nutsedge leaves. There are also a number of reports that some systemic herbicides, apparently acting as synthetic auxins, stimulate ethylene production in treated plants and that their physiological effects can be attributed in part to the effects of this natural hormone (1-3, 16, 20, 28, 32). In this report the action of dicamba is examined by comparing the effects of the herbicide and ethylene on protein synthesis and cell membrane permeability. MATERLALS AND METHODSCommon purslane (Portulaca oleracea L.) seeds were sown in sand in 10-cm plastic pots placed in trays filled with Hoagland's nutrient solution (19) for subirrigation. The plants were grown in a growth room under a 14-hr light (1100 ft-c) and 10-hr dark period at a continuous temperature of 25 + 1 C and 60% relative humidity. At 2 weeks of age the plants were thinned to three plants per pot.Dicamba and Ethylene Treatments. At 4 weeks of age the purslane plants were treated with various levels of dicamba dissolved in water. Foliar application was performed by placing 20-Mxl droplets of a desired concentration on each of five fully developed leaves of ea...

show abstract

A survey of herbicides for their effect upon protein synthesis.

Cited by 98 publications

References 1 publication

MICROTUBULE BIOGENESIS AND CELL SHAPE IN OCHROMONAS

MICROTUBULE BIOGENESIS AND CELL SHAPE IN OCHROMONAS

Effect of Trifluralin on Growth, Morphology, and Nucleic Acid Synthesis

Participation of Ethylene in Common Purslane Response to Dicamba

Contact Info

Product

Resources

About